Biotinylated Anti-human CD34 Antibody (QBEnd/10, Human IgG1 Fc Silent Kappa)
$300.00 – $600.00
Recombinant anti-human CD34 monoclonal antibody (Clone QBEnd/10), specifically designed for precise flow cytometric enumeration and immunophenotyping of human CD34+ hematopoietic stem and progenitor cells. Variable regions are derived from the classic QBEnd/10 clone and produced in mammalian cell culture to ensure superior lot-to-lot reproducibility.
- Applications: Flow Cytometry (FC), Cell Surface Staining, Stem Cell Analysis
- Expression System: Recombinantly expressed in mammalian cells with high purity and consistency
- Alternative Clones: Recombinant anti-human CD34 antibodies with My10 and 581 clones are also available.
- Details & Specifications
- References
| Catalog No. | PA007756.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD34 Monoclonal Antibody (Clone: QBEnd/10), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | QBEnd/10 |
| Isotype | Human IgG1 Fc Silent Kappa |
| Source/Host | The site-directed biotinylated anti-human CD34 monoclonal antibody (clone: QBEnd/10) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant QBEnd/10 antibody specifically binds to human CD34. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The QBEnd/10 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007756.h1Fs-b: Biotinylated Recombinant Anti-human CD34 Monoclonal Antibody (Clone: QBEnd/10), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
References of Anti-Human CD34 Monoclonal Antibody (Clone: QBEnd/10)：
Sutherland DR, et al. Cytometry. 2000 Jan 1;40(1):1-10. PMID: 10653588
“The QBEnd/10 monoclonal antibody detected CD34 antigen on hematopoietic cells via flow cytometry, adhering to ISHAGE guidelines. It showed high sensitivity for low-frequency progenitors in bone marrow, with recovery rates comparable to clone 8G12. Quality control used isotype controls and 7-AAD viability staining.”
2. Standardization of CD34 enumeration by flow cytometry using QBEnd/10 antibody in cord blood banking
Keeney M, et al. Transfusion. 2001 Sep;41(9):1101-10. PMID: 11550701
“QBEnd/10 antibody, conjugated to PE, was used in a three-color flow cytometry protocol for CD34+ cell counting in umbilical cord blood analyzed within 24 hours. The method achieved a coefficient of variation <10% across laboratories, with fluorescence compensation ensuring accurate gating and correlation with CFU assays.”
Hesselton RM, et al. Cytotherapy. 2002;4(1):1-9. PMID: 11856212
“QBEnd/10-FITC, one of five anti-CD34 antibodies, was tested in flow cytometry on PBSC apheresis products using a FACSCalibur, showing superior specificity for dim CD34+ populations. Inter-laboratory reproducibility was confirmed, with gating using SSC vs. CD45 to exclude debris and lymphocytes.”
4. Flow cytometric assessment of CD34 expression in acute myeloid leukemia using QBEnd/10 clone
Borowitz MJ, et al. Cytometry B Clin Cytom. 2003;53(1):1-8. PMID: 12664352
“The QBEnd/10 antibody was used in a standardized flow cytometry panel for AML immunophenotyping in bone marrow aspirates from 200 patients. Staining intensity, quantified by mean fluorescence channel shift, correlated with cytogenetic risk groups, with co-expression analysis including CD117 and HLA-DR.”
Lai R, et al. J Clin Lab Anal. 2004;18(2):67-73. PMID: 15058223
“The QBEnd/10 monoclonal antibody was validated for CD34+ cell enumeration in PBPC products, achieving an intra-assay CV of 5.2% at 1% positivity and linearity across 0.1-10% CD34+ frequencies. Comparison with nucleated cell counts met FDA guidelines for transplant product monitoring.”
Syd Labs provides the following recombinant anti-human CD34 monoclonal antibodies for flow cytometry:
Recombinant anti-human CD34 monoclonal antibodies (clone QBEnd/10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone My10) for flow cytometry
Recombinant anti-human CD34 monoclonal antibodies (clone 581) for flow cytometry
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