Biotinylated Anti-human CD20 Antibody (2H7, Human IgG1 Fc Silent Kappa)
$65.00 – $130.00
Biotinylated recombinant anti-human CD20 monoclonal antibody (Clone 2H7), engineered with a Human IgG1 Fc-silent domain to eliminate non-specific Fc receptor binding during flow cytometric analysis. Features site-directed biotinylation via a C-terminal Avi tag on the heavy chain, with variable regions derived from anti-human CD20 clone (2H7) and expressed in mammalian cells.
- Conjugation & Purity: Biotinylated (Site-directed via C-terminal Avi-tag), > 95% by SDS-PAGE
- Applications: Flow Cytometry (FC) via Streptavidin Conjugates, Cell Surface Staining, B Cell Depletion Monitoring
- Alternative Formats: Unconjugated human IgG1 Fc-silent, mouse IgG1, and other recombinant versions of clone 2H7 as well as B9E9 and 1F5 clones are also available at Syd Labs and Ushelf.
- Details & Specifications
- References
| Catalog No. | PA007746.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | 2H7 |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human CD20 monoclonal antibody (clone: 2H7) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant 2H7 antibody specifically binds to human CD20. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The 2H7 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007746.h1Fs-b: Biotinylated Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
References of Anti-Human CD20 Monoclonal Antibody (Clone: 2H7):
Hörner, S., et al. Cancers (Basel). 2022 Aug 12;14(16):3941. doi: 10.3390/cancers14163941. PMID: 36010934
“The study utilized hybridoma-derived antibodies 2H7, 4G7, and Apo-1 for quantification. The bispecific antibodies (bsAbs) combined the CD95 agonist Apo-1 with either the anti-CD20 antibody clone 2H7 or the anti-CD19 clone 4G7 in F(ab)2 and single-chain formats. The CD95xCD20 bsAb demonstrated remarkable potency with IC50 values as low as 25 pM, despite the relatively low affinity of the anti-CD20 mAb clone 2H7 as a single chain. Expression of CD20, CD19, and CD95 on various cell lines was quantified using flow cytometry with QIFIKIT calibration beads, and binding of bsAbs to Daudi and Jurkat cells was assessed via flow cytometry.”
Boldison, J., et al. Diabetologia. 2019 Sep;62(9):1669-1678. doi: 10.1007/s00125-019-04974-y. PMID: 31037349
“In this study, female VH125.hCD20/NOD mice (6-8 weeks old) were treated with either anti-hCD20 antibody (clone 2H7) or control IgG2b antibody (clone MPC-11). Over a 12-week observation period, 48% of control-treated mice developed diabetes compared to 15% of 2H7-treated mice. The study involved injecting groups of 6- to 8-week-old VH125.hCD20/NOD mice with 2H7 anti-CD20 or IgG isotype control, followed by analysis of spleen and pancreatic lymph node (PLN) tissue for anti-insulin B cells post-treatment.”
For more technical references or data sheets regarding the Anti-Human CD20 Monoclonal Antibody (Clone: 2H7) formats, please contact our scientific support team at message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD20 monoclonal antibodies:
Recombinant Anti-human CD20 monoclonal antibody (Clone: 2H7)
Recombinant Anti-human CD20 monoclonal antibody (Clone: B9E9)
Recombinant Anti-human CD20 monoclonal antibody (Clone: 1F5)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD20 monoclonal antibodies:
Recombinant Anti-mouse CD20 monoclonal antibody (Clone: 2H7)
Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD20 monoclonal antibody (Clone: 2H7) for flow cytometry
Recombinant Anti-human CD20 monoclonal antibody (Clone: B9E9) for flow cytometry
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