Biotinylated Anti-human CD19 Antibody (B43, Human IgG1 Fc Silent Kappa)
$80.00 – $160.00
Biotinylated recombinant anti-human CD19 monoclonal antibody (Clone B43), engineered with a Human IgG1 Fc-silent domain to eliminate non-specific Fc receptor binding during flow cytometric analysis. Features site-directed biotinylation via a C-terminal Avi tag on the heavy chain, with variable regions derived from anti-human CD19 clone (B43) and expressed in mammalian cells.
- Conjugation & Purity: Biotinylated (Site-directed via C-terminal Avi-tag), > 95% by SDS-PAGE
- Applications: Flow Cytometry (FC) via Streptavidin Conjugates, Cell Surface Staining, B Cell Profiling
- Alternative Formats: Unconjugated human IgG1 Fc-silent, mouse IgG1, and other recombinant versions of clone B43 as well as SJ25C1, FMC63, and 4G7 clones are also available at Syd Labs and Ushelf.
- Details & Specifications
- References
| Catalog No. | PA007744.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | B43 |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated recombinant anti-human CD19 monoclonal antibody (clone: B43) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant B43 antibody specifically binds to the human CD19 protein. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The B43 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007744.h1Fs-b: Biotinylated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
The B43 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.
References of Anti-Human CD19 Monoclonal Antibody (Clone: B43):
Caron, A. W., et al. BMC Biotechnol. 2009 May;9(42):42. PMID: 19432976
“The light chain of the B43 antibody (anti-human CD19) was replaced in the pMPGB43k expression vector, which carries an enhanced CMV enhancer/promoter and a hygromycin resistance gene, to construct a new vector for recombinant protein expression. The B43 antibody (anti-human CD19 mAb) coding sequence was modified via PCR and cloned into the PmeI site of pMPGB34k, with a BamHI site added for further modifications. The study also isolated the IGF-1 coding sequence from murine cDNA for constructing pMPG-IGF-E5.”
Ishido, S., et al. J Virol. 2000 Jun;74(11):5300-9. PMID: 10799607
“The B43 antibody (anti-human CD19) was used alongside W6/32 (MHC class I), RPA-T8 (CD8), M-A712 (transferrin receptor), and TU39 (MHC class II) for fluorescence-activated cell sorting and confocal immunofluorescence analyses. K3 and K5 proteins specifically downregulated MHC class I without affecting MHC class II, immunoglobulin M, or CD19 surface levels, as confirmed by these antibodies.”
3. Large scale manufacturing of B43(anti-CD19)-genistein for clinical trials in leukemia and lymphoma
Myers, D. E., et al. Leuk Lymphoma. 1998 Apr;29(3-4):329-38. PMID: 9684930
“The B43(anti-human CD19 antibody)-genistein immunoconjugate was manufactured at large scale for clinical trials in leukemia and lymphoma. The B43 antibody was purified from ascites fluid using protein A affinity chromatography, and its conjugation to genistein was optimized for high yield. Flow cytometry confirmed effective binding of B43-genistein to CD19+ leukemic cells, with cytotoxicity tested against leukemia cell lines.”
Kreitman, R. J., et al. Clin Cancer Res. 1997 Mar;3(3):325-37. PMID: 9815689
“The B43(anti-human CD19)-pokeweed antiviral protein (PAP) immunotoxin was evaluated in cynomolgus monkeys for pharmacokinetics, immunogenicity, and toxicity. B43-PAP, constructed by linking the anti-CD19 monoclonal antibody B43 to PAP, killed >99.9% of primary leukemic progenitor cells from B-lineage ALL patients. The study revealed dose-limiting renal and cardiac toxicity in mice and severe renal tubular necrosis in monkeys.”
Myers, D. E., et al. Proc Natl Acad Sci U S A. 1995 Oct;92(21):9575-9. PMID: 7568175
“The B43 monoclonal antibody (anti-human CD19 mAb) was used to cross-link membrane-associated CD19, activating the LYN kinase signaling pathway and inducing apoptosis in B-lineage lymphoma cells in a p53- and Bcl-2-independent manner. The CD19-LYN complex was immunoprecipitated using B43 antibody, and flow cytometry confirmed high-level CD19 expression on lymphoma cells.”
For more technical references or data sheets regarding the Recombinant Anti-Human CD19 Monoclonal Antibody (Clone: B43) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)
Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)
Recombinant Anti-human CD19 monoclonal antibody (Clone: 4G7)
Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry
Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: 4G7) for flow cytometry
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