Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Human IgG1 Fc Silent Kappa PA007238.h1Fs Syd Labs

Anti-mouse CD3e Antibody for Flow Cytometry | PA007238.h1Fs

$40.00 – $70.00

Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007238.h1Fs
Product Name	Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 3, CD3D, CD3E, CD3G
Summary	The recombinant anti-mouse CD3e monoclonal antibody (clone: 145-2C11) was produced in mammalian cells.
Clone	145-2C11.
Isotype	Human IgG1 kappa.
Source/Host	The recombinant anti-mouse CD3e monoclonal antibody (clone: 145-2C11) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant 145-2C11 antibody for Flow Cytometry specifically binds to the mouse T cell receptor CD3e.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11) for Flow Cytometry, Human IgG1 Fc Silent Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007238.h1Fs: Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11) for Flow Cytometry, Human IgG1 Fc Silent Kappa

The 145-2C11 antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes.

References of Anti-Mouse CD3e Monoclonal Antibody (Clone: 145-2C11)

1. A novel trivalent non-Fc anti-CD3 Collabody preferentially induces Th1 cell apoptosis in vitro and long-lasting remission in recent-onset diabetic NOD mice
Huang CC, et al.Front Immunol. 2023 Aug 3;14:1201853. PMID: 37600814
“The V H and V L domains of the hamster anti-mouse CD3 ε antibody (clone 145-2C11) were grafted onto their respective human CH 1 and kappa light chain…Purified T cells were incubated with threefold serial dilutions of each anti-CD3 antibody ranging from 0 to 300 nM (145-2C11) or 600 nM (h145F(ab’) 2, h145chIgGAA, and h145CSA) for 1 h on ice, followed by staining with FITC-conjugated goat anti-hamster IgG (145-2C11) or Alexa Fluor 647-conjugated goat anti-human IgG (H+L) (h145F(ab’) 2, h145chIgGAA, and h145CSA)…Purified total T cells were plated at 5 × 10 4 cells/well in a 96-well plate and incubated at 37°C in RPMI-1640 medium supplemented with l-glutamine, 1 mM sodium pyruvate, 50 µM β-mercaptoethanol, and 10% heat-inactivated fetal bovine serum (complete RPMI) in the presence of increasing concentrations of the 145-2C11, h145F(ab’) 2, h145chIgGAA, or h145CSA for 24 h…Naïve CD4 + T, activated CD4 + T, Th1, and Treg cells (2 × 10 4 cells/well) were incubated in a 96-well plate in the presence of serial logarithmic dilutions of 145-2C11, h145chIgGAA, or h145CSA for 6, 12, or 24 h…Cells were stained with Alexa Fluor 647-conjugated anti-IFN-γ (clone XMG1.2), -CD3 (clone 145-2C11), APC-conjugated -CD25 (clone 3C7), FITC-conjugated Annexin V, 7-AAD, biotin-conjugated anti-FasL (clone MFL3), and APC-conjugated streptavidin were all purchased from…”

2. Murine RAW Macrophages Are a Suitable Model to Study the CD3 Signaling in Myeloid Cells
Ocaña-Guzmán R, et al.Cells. 2022 May 13;11(10):1635. PMID: 35626672
“PE anti-mouse CD3 epsilon 145-2C11 100308…PE Armenian Hamster IgG isotype control…PE/Cy7 anti-mouse CD3 epsilon 145-2C11 100320…PE Armenian Hamster IgG isotype control HTK888 400922…Ultra-LEAF(TM) Purified anti-mouse CD3 antibody.”

3. A humanized CD3ε-knock-in mouse model for pre-clinical testing of anti-human CD3 therapy
Crespo J, et al.PLoS One. 2021 Feb 17;16(2):e0245917. PMID: 33596227
“Antibodies used for in vitro functional studies or in vivo treatment were anti-human CD3 (Clone OKT3), anti-mouse CD3 ε (Clone 145-2C11) and anti-mouse TCR β …Wells were coated overnight at 4°C with anti-human CD3 (OKT3, 1.0 μ g/mL), anti-mouse CD3 (145-2C11, 1.0 μ g/mL), or anti-mouse TCR β (1.0 μ g/mL), and their respective isotype controls (mouse IgG2a or Armenian hamster IgG)…Although T cells from WT mice only upregulated CD69 expression in response to anti-mouse CD3 (145-2C11) activation, T cells from huCD3 ε HET mice responded to both 145-2C11 and anti-human CD3 (OKT3) demonstrating that both human and mouse CD3 ε are functional…In line with these data, the intensity of CD3 staining using 145-2C11 antibody was decreased in huCD3 ε HET T cells compared to WT cells…Finally, we tested the consequences of injecting anti-CD3 (OKT3 or 145-2C11) antibodies into naïve huCD3 ε HET or WT mice.”

4. Entire CD3ε, δ, and γ humanized mouse to evaluate human CD3-mediated therapeutics
Ueda O, et al.Sci Rep. 2017 Apr 3;7:45839. PMID: 28368009
“For splenic T cell–stimulation via CD3, splenocyte cells were cultured on a plate coated with antibody to mouse Cd3 (145-2C11) or antibody to human CD3 (HIT3a)…Bispecific antibody against hGPC3 and mCD3 was generated by Fab arm exchange using anti-GPC3 antibody (GC33) and anti-mouse CD3 antibody (2C11)…Flow cytometric analysis demonstrated that human CD3+ cells were detected in splenocytes in three lines of human CD3 EDG–replaced mice (1C3, 8I12, and 4HH3)…Primary splenocytes were isolated from each mouse by mechanical dissociation followed by red blood–cell lysis…These splenocytes were stained with the following monoclonal antibodies: FITC labeled anti-human CD3 (clone; UCHT1), FITC labeled anti-mouse CD3 (clone; 17A2), APC labeled CD4 (clone; RM4-5), PE labeled CD8 (clone; 53-6.7), APC-Cy7 labeled CD45R/B220 (clone; RA3-6B2).”

5. Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody
Sugita S, et al.Arthritis Res Ther. 2017 Jul 25;19(1):176. PMID: 28743289
“Mouse pan-T cells isolated from C57BL/6JJcl donors were incubated with 1 μg/ml of Dow2 or a control anti-mouse CD3 Ab (clone 17A2 or clone 145-2C11) at 4 °C for 30 min…Dow2-pretreated pan-T cells that were incubated with 145-2C11 were also incubated with the secondary Alexa Fluor 488 Ab…Using flow cytometry, we first determined whether Dow2 can bind CD3 on mouse T cells by comparing its staining profile with two control anti-mouse CD3 Abs, 17A2 and 145-2C11…T cells stained with Dow2 demonstrated a clear separation from the control T-cell population, comparable to the separation observed for T cells stained with 17A2 or 145-2C11…T cells preincubated with Dow2 had reduced binding with 145-2C11, suggesting that Dow2 is similarly able to bind mouse CD3 ε.”

6. Differential depletion of total T cells and regulatory T cells and prolonged allotransplant survival in CD3ε humanized mice treated with polyclonal anti human thymocyte globulin
Buszko M, et al.PLoS One. 2017 Mar 1;12(3):e0173088. PMID: 28257450
“In murine research, a monoclonal anti-mouse CD3 ε mAb (clone 145-2C11) is used as a surrogate for OKT3 and it has been shown that in vivo administration of high doses of this mAb prolongs transplant survival…In contrast, low doses of 145-2C11 reversed spontaneous diabetes in NOD mice and decreased lipid accumulation in LDLr -/- mice, thus inhibiting atherosclerosis progression…Anti-mouse CD3 mAb (clone: 145-2C11) was produced in-house or purchased from R&D…For flow cytometric analysis cells were stained 20–30 min at 4°C in the dark…In vitro stimulation assay 2 x 10^5 splenocytes labeled with 2 μM CFSE were stimulated with plate-bound anti-mouse or human CD3, or ATG in complete media.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD3e Monoclonal Antibody (Clone: 145-2C11) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.