Catalog No.	PA007199.r2b
Product Name	Anti Mouse CD3 Antibody, clone 17A2 | PA007199.r2b
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 3, CD3D, CD3E, CD3G.
Summary	The anti-mouse CD3 antibody (clone: 17A2) was produced in mammalian cells.
Clone	17A2
Isotype	Rat IgG2b kappa
Specificity/Sensitivity	The in vivo grade recombinant rat monoclonal antibody (clone: 17A2) specifically binds to the mouse T cell receptor CD3.
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD3 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The 17A2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007199.r2b: Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Rat IgG2b Kappa, In vivo Grade

In vivo Grade Recombinant Anti-mouse CD3 Rat IgG2b Kappa Monoclonal Antibody (Clone: 17A2) was produced in mammalian cells. The ‌Anti-Mouse CD3 Monoclonal Antibody (Clone 17A2)‌ is a high-affinity, rigorously validated reagent designed for precise detection and quantification of CD3ε, a critical pan-T cell marker in murine models. This antibody demonstrates exceptional specificity for mouse CD3, validated in applications including ‌flow cytometry (FACS)‌, ‌immunohistochemistry (IHC)‌, ‌immunofluorescence (IF)‌, and ‌Western blotting‌, ensuring reliable performance across diverse experimental setups. Clone 17A2 targets the ε-chain of the CD3 complex, enabling accurate identification of T cell populations in lymphoid tissues, peripheral blood, or cultured cells. Ideal for studies in immunology, autoimmune disorders, and oncology.

References for Anti-mouse CD3 Antibody(17A2)：

1、Urolithin A ameliorates experimental autoimmune encephalomyelitis by targeting aryl hydrocarbon receptor.
Li X., et al. EBioMedicine. 2021 Feb;64:103227. doi: 10.1016/j.ebiom.2021.103227. PMID: 33530002
“Consistent to the reduction of T cells numbers detected in the CNS, the proportion of CD3+ and CD4+ cells in the URA-administered group was also remarkably reduced in the peripheral immune system. …However, its role in autoimmune diseases is largely unknown. Here, for first time, we demonstrate the therapeutic effect of URA in an experimental autoimmune encephalomyelitis (EAE) animal model. …Histological evaluation showed that significantly fewer inflammatory cells, decreased demyelination, lower numbers of M1-type microglia and activated DCs, as well as reduced infiltrating Th1/Th17 cells were present in the central nervous system (CNS) of the URA-treated group. …Moreover, we confirmed URA inhibited pathogenicity of Th17 cells in adoptive EAE. …Multiple Sclerosis (MS) is characterized by inflammatory cell infiltration and demyelination.”

2、Metabolic Dynamics of In Vitro CD8+ T Cell Activation.
Edwards-Hicks J., et al. Metabolites. 2020 December 28;11(1):12. doi: 10.3390/metabo11010012. PMID: 33379404
“CD8+ T cells were activated at 1.5 × 106/mL for 48 h using plate-bound anti-CD3 (5 μg mL−1; InVivoMAb anti-mouse CD3, BioXCell no. BE0002) and soluble anti-CD28 (0.5 μg/mL; InVivoMAb anti-mouse CD28, BioXcell no. BE0015) in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal calf serum (Gibco), 4 mM L-glutamine, 1% penicillin–streptomycin, hrIL-2 (human recombinant interleukin 2, 100 U ml−1, Peprotech), and 55 μM beta-mercaptoethanol at 37 °C in a humidified incubator containing atmospheric oxygen supplemented with 5% CO2. …Cells density was determined immediately before sampling by counting in Neubauer chamber. …The FIA method has been adapted from a previously published method by Fuhrer et al. …Just prior to analysis, samples were resuspended in 20 µL 90:10 acetonitrile:water. …When activated from their naïve state, T cells undergo a complex transition, including major metabolic reprogramming.”

3、LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6.
Youn Soo Choi et al. Nat Immunol. 2015 Sep;16(9):980-90. doi: 10.1038/ni.3226. PMID: 26214741
“Follicular helper T cells (TFH cells) are specialized effector CD4+ T cells that help B cells develop germinal centers (GCs) and memory. …However, the transcription factors that regulate the differentiation of TFH cells remain incompletely understood. …Here we report that selective loss of Lef1 or Tcf7 (which encode the transcription factor LEF-1 or TCF-1, respectively) resulted in TFH cell defects, while deletion of both Lef1 and Tcf7 severely impaired the differentiation of TFH cells and the formation of GCs. …Forced expression of LEF-1 enhanced TFH differentiation. …First, they established the responsiveness of naive CD4+ T cells to TFH cell signals.”

4、Bcl6 middle domain repressor function is required for T follicular helper cell differentiation and utilizes the corepressor MTA3.
Nance J.P., et al. Proc Natl Acad Sci U S A. 2015 Oct 27;112(43):13324-9. doi: 10.1073/pnas.1507312112. PMID: 26460037
“Currently, the mechanisms by which Bcl6 directs Tfh cell differentiation is unclear. …T follicular helper (Tfh) cells are essential providers of help to B cells. …Mimicked acetylation of the Bcl6 middle domain (K379Q) in CD4 T cells results in significant reductions in Tfh differentiation in vivo. …This was a result of the failure of Bcl6 K379Q to recruit metastasis-associated protein 3 (MTA3). …In addition to Prdm1, we found that Bcl6 recruits MTA3 to multiple genes involved in Tfh cell biology, including genes important for cell migration, cell survival, and alternative differentiation pathways.”

5、Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.
Hu S., et al. PLoS One. 2014 Aug 18;9(8):e105219. doi: 10.1371/journal.pone.0105219. PMID: 25133679
“Lymphocytes were stimulated in co-cultures using a mixture of monoclonal antibodies to CD3 (5 µg/ml; clone 17A2) and CD28 (5 µg/ml; clone 37.5; BioXcell, West Lebanon, NH). …Luciferase activity levels from luciferase (luc)+ murine NSCs were used as an indicator of cell proliferation. …NSC and T lymphocyte cultures were pulsed with 10 µM BrdU 72 h after co-cultures were instituted. …The ability of neural stem/progenitor cells (NSCs) to self-renew, migrate to damaged sites, and differentiate into neurons has renewed interest in using them in therapies for neurodegenerative disorders. …This differential lymphocyte effect on proliferation was confirmed by decreased BrdU uptake by NSC cultured with activated CD8 T-cells.”

6、Bcl6 expressing follicular helper CD4 T cells are fate committed early and have the capacity to form memory.
Yang JA., et al. J Immunol. 2013 Apr 15;190(8):4014-26. doi: 10.4049/jimmunol.1202963. PMID: 23487426
“Follicular helper CD4 T (Tfh) cells are a distinct type of differentiated CD4 T cells uniquely specialized for B cell help. …Using cell transfer approaches at early time points after an acute viral infection, we demonstrate that early Tfh cells and Th1 cells are already strongly cell fate committed by day 3. …Nevertheless, Tfh cell proliferation was tightly regulated in a TCR-dependent manner. …The Tfh cells still depend on extrinsic cell fate cues from B cells in their physiological in vivo environment. …These data support the hypothesis that CD4 and CD8 T cells share core aspects of a memory cell precursor gene expression program involving Bcl6, and a strong relationship exists between Tfh cells and memory CD4 T cell development.”

Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Anti-human CD3 monoclonal antibody (Clone: OKT3)
Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)

Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)

Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry

Anti-mouse CD3 Antibody(17A2) from: In vivo Grade Recombinant Anti-mouse CD3 Rat IgG2b Kappa Monoclonal Antibody (Clone: 17A2): PA007199.r2b Syd Labs

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