Anti-Human EGFR Antibody (Clone:225) | PA007575.m1
$150.00 – $900.00
Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007575.m1 |
|---|---|
| Product Name | In Vivo Grade Recombinant Anti-human EGFR Monoclonal Antibody (Clone: 225), Mouse IgG1 Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | ErbB, EC 2.7.10, EC 2.7.10.1, EGFR, mENA, LEGFR, ERBB, ERBB1, HER1, PIG61, NISBD2 |
| Clone | 225. |
| Isotype | Mouse IgG1 Kappa. |
| Source/Host | The anti-human EGFR monoclonal antibody (clone: 225) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant mouse monoclonal antibody (clone: 225) specifically binds to human EGFR. |
| Applications | ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human EGFR protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The in vivo grade recombinant anti-human EGFR monoclonal antibody of clone 225 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007575.m1:In Vivo Grade Recombinant Anti-human EGFR Monoclonal Antibody (Clone: 225), Mouse IgG1 Kappa
Recombinant Mouse IgG1 Monoclonal Antibody.
References of Anti-Human EGFR Monoclonal Antibody (Clone: 225)
1. Near-infrared fluorescent molecular probes with cetuximab in the in vivo fluorescence imaging for epithelial ovarian cancer.
Chen, Y., et al.J Ovarian Res. 2024 Nov 14;17(1):138. doi: 10.1186/s13048-024-01547-5. PMID: 39482752
“Cetuximab (C225), a monoclonal antibody targeting EGFR, was conjugated with Cy7 for in vivo fluorescence imaging in a subcutaneous SKOV3-Luc xenograft model of epithelial ovarian cancer (EOC) with EGFR overexpression. NIRF imaging with 30 µg cetuximab-Cy7 enabled detection of tumor lesions, with ex vivo analysis showing fluorescence primarily in tumors and lungs compared to controls (PBS, free Cy7, and isotype IgG-Cy7).”
2. Inhibition of N-acetylglucosaminyltransferase V enhances the cetuximab-induced radiosensitivity of nasopharyngeal carcinoma cells likely through EGFR N-glycan alterations.
Chen, Y., et al.Oncol Lett. 2017 Aug;14(2):1521-1528. doi: 10.3892/ol.2017.6319. PMID: 28535302
“Cetuximab (C225), an EGFR inhibitor, enhanced radiosensitivity in nasopharyngeal carcinoma (NPC) cells when combined with N-acetylglucosaminyltransferase V (GnT-V) downregulation, which altered EGFR N-glycans. This combination reduced phospho-Akt and phospho-PI3K signaling and decelerated tumor growth in vivo, improving outcomes in CNE-2 cells compared to irradiation or cetuximab alone.”
3. Enhanced cytotoxic activity of cetuximab in EGFR-positive lung cancer by conjugating with gold nanoparticles.
Kim, H., et al.Sci Rep. 2014 Dec 15;4:7808. doi: 10.1038/srep07808. PMID: 25502402
“Cetuximab (C225), targeting EGFR-positive non-small cell lung cancer (NSCLC), was conjugated with 14 nm gold nanoparticles (AuNPs), enhancing cytotoxicity in A549 tumor-bearing mice both in vitro and in vivo. Compared to controls (saline, C225 alone, IgG-AuNPs), C225-AuNPs showed superior therapeutic potential for EGFR-positive NSCLC.”
4. In vivo and ex vivo proofs of concept that cetuximab conjugated vitamin E TPGS micelles increases efficacy of delivered docetaxel against triple negative breast cancer.
Yang, J., et al.Biomaterials. 2015 Aug;58:1-11. doi: 10.1016/j.biomaterials.2015.04.048. PMID: 26052086
“Cetuximab-conjugated vitamin E TPGS micelles enhanced docetaxel delivery in a triple-negative breast cancer (TNBC) xenograft model using EGFR-overexpressing MDA-MB-231/Luc cells in SCID mice. In vivo biodistribution via IVIS® imaging showed improved tumor targeting, requiring a lower M−NP dosage to achieve tumor inhibition comparable to free drug.”
For more technical references or data sheets regarding the Recombinant Anti-Human EGFR Monoclonal Antibody (Clone: 225) formats, or related products from Syd Labs, please contact our scientific support team at message@sydlabs.com.
In Vivo Grade Recombinant Anti-human EGFR Monoclonal Antibody (Clone: 225), Mouse IgG1 Kappa from: In Vivo Grade Recombinant Anti-human EGFR Monoclonal Antibody (Clone: 225), Mouse IgG1 Kappa: PA007575.m1
You may also like…
-
Select optionsQuick View
