Description

PA007567.r2a: In Vivo Grade Recombinant Anti-mouse CD29 (Integrin beta 1) Monoclonal Antibody (Clone: KMI6), Rat IgG2a Kappa

Recombinant rat IgG2a Monoclonal Antibody.

References of Anti-Mouse CD29 (Integrin β1) Monoclonal Antibody (Clone: KMI6)

1. Mucosally transplanted mesenchymal stem cells stimulate intestinal healing by promoting angiogenesis.
Manieri, N. A., et al.J Clin Invest. 2015 Sep;125(9):3606-18. doi: 10.1172/JCI81423. PMID: 26280574
“rabbit monoclonal anti-Ki67 (clone SP6; Vector), rat monoclonal anti-CD29 (clone KMI6; BD Biosciences), rat monoclonal anti-CD31 (clone MEC 13.3; BD Biosciences), rat monoclonal anti-CD44 (clone IM7; BD Biosciences). …The dissected colons were flushed with PBS and then OCT compound and opened longitudinally. …A construct of the entire wound bed was produced, and the total number of VEGF-positive cells was recorded per wound bed. …The 2-nitroimidazole compound EF5 was a gift from Cameron Koch (University of Pennsylvania) produced by the National Cancer Institute. …Tivozanib (1 µg/kg body weight) was diluted into 300 µl corn oil and orally gavaged into WT mice once daily.”

2. PDGFRα and CD51 mark human nestin+ sphere-forming mesenchymal stem cells capable of hematopoietic progenitor cell expansion.
Pinho, S., et al.J Exp Med. 2013 Jul 1;210(7):1351-67. doi: 10.1084/jem.20122252. PMID: 23776077
“Other conventional mesenchymal lineage markers were heterogeneously expressed (CD29, CD44, CD61, CD130, and P75) or restricted to a small subset (<15%) of Nestin+ cells (CD10, CD90, CD166, and CD133). …CD10 (clone SN5c/L4-1A1) was purchased from Santa Cruz Biotechnology, Inc. CD31 (clone MEC13.3), CD105 (clone MJ7/18), and CD48 (clone HM48-1) were from purchased from BioLegend, whereas CD29 (clone KMI6) and CD146 (clone ME-9F1) were purchased from BD. …Human fetal bones and HA/TCP grafts were fixed with 4% PFA for 2 h at 4°C, partially decalcified with 0.25 M EDTA for 2-3 d, and cryoprotected with 15-30% sucrose. …For renal capsule collagen graft, five thousand freshly sorted cells or single spheres were gently resuspended in 15 µl of a collagen (BD) mixed with 2% of 1N NaOH and 10% of 10× PBS. …As the sponges expanded, they incorporated the cells.”

3. Igf2bp1 is required for full induction of Ptgs2 mRNA in colonic mesenchymal stem cells in mice.
Manieri, N. A., et al.Gastroenterology. 2012 Jul;143(1):110-21.e10. doi: 10.1053/j.gastro.2012.03.037. PMID: 22465430
“Instead, Ptgs2 high-expressing cells showed dual labeling for multiple cMSC markers including CD44, CD29, and CD54 (Figure 3B). …The accepted markers for MSCs are a constellation of positive markers including CD29, CD44, and CD54, as well as negative lineage markers. …rat monoclonal anti-CD29 (KMI6; BD Biosciences, San Jose, CA), rat monoclonal anti-CD31 (MEC 13.3; BD Biosciences). …All primary antibodies were used at 1:100 dilution except anti-Ptgs2 (1:5,000 dilution). For Ptgs2, claudin-4, and ZO-1 staining, sections from unfixed colons were acetone-fixed (Ptgs2) or methanol-fixed (ZO-1 and claudin-4) for 5 minutes, rinsed in PBS, blocked, and incubated with primary and secondary antibodies without antigen retrieval. …Colons were minced and treated with collagenase type 1 (from Clostridium histolyticum; Invitrogen).”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD29 (Integrin β1) Monoclonal Antibody (Clone: KMI6) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse CD29 (Integrin beta 1) Monoclonal Antibody (Clone: KMI6), Rat IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse CD29 (Integrin beta 1) Monoclonal Antibody (Clone: KMI6), Rat IgG2a Kappa: PA007567.r2a