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Anti-human CD19 scFv (FMC63)

Name: Anti-human CD19 scFv | Ushelf
Brand: Syd Labs
SKU: PA007422.scFv
Price: 650 USD
Availability: InStock
Rating: 5 (15 reviews)

Catalog No.: PA007422.scFv | Isotype: scFv. | Flow Cytometry

$650.00

Recombinant anti-human CD19 monoclonal antibody (Clone FMC63), engineered for flow cytometry applications and recombinantly expressed in mammalian cells. Variable regions are derived from the gold-standard mouse anti-human CD19 clone FMC63, providing high-affinity targeting of human B-lineage cells.

Endotoxin Level: < 1.0 EU/mg
Applications: Flow Cytometry (FC), Cell Surface Staining, CAR-T Research
Alternative Formats: Recombinant human IgG1, mouse IgG1/IgG2a, and in vivo grade CD19 antibody clones (SJ25C1, B43, 4G7) are also available.

Details & Specifications
References

Catalog No.	PA007422.scFv
Product Name	Recombinant Anti-human CD19 scFv (Clone: FMC63)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	FMC63
Isotype	scFv
Source/Host	The recombinant anti-human CD19 scFv (clone FMC63) with His-tag at the C-terminus is produced in mammalian cells.
Specificity/Sensitivity	The recombinant anti-human CD19 scFv (clone: FMC63) specifically binds to the human CD19 protein.
Applications	ELISA and flow cytometry.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The FMC63 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007422.scFv: Recombinant Anti-human CD19 scFv (Clone: FMC63)

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

The FMC63 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.

References of Anti-Mouse CD19 Monoclonal Antibody (Clone: 1D3)
1. Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus.

Muller N, et al. Mol Ther Methods Clin Dev. 2022 Jul 8;26:131-141. PMID: 35755944

“Cells were stained with FITC-conjugated anti-CD19 mAb (clone 1D3) and analyzed by flow cytometry to monitor B cell depletion. The depletion efficiency was >95% in peripheral blood as determined by flow cytometry using 1D3 antibody. Peripheral blood was collected and stained with anti-CD19 (1D3) and anti-CD3 antibodies. Flow cytometry was performed on a FACSCalibur using CellQuest software. B cell depletion was confirmed by the absence of CD19+ cells in flow analysis.”

2. Therapeutic efficacy of anti-CD19 CAR-T cells in a mouse model of systemic lupus erythematosus.

Ma J, et al. Cell Mol Immunol. 2021 Aug;18(8):1896-1903. PMID: 32472023

“For flow cytometry, cells were stained with anti-CD19 (clone 1D3) antibody to identify B cells. The percentage of CD19+ B cells was significantly reduced in treated mice. Spleen cells were isolated and stained with 1D3 antibody for CD19 expression. Data were acquired on a BD FACSCanto II flow cytometer. Treatment led to long-term B cell depletion as measured by flow.”

3. Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells.

Kochenderfer JF, et al. Blood. 2010 Nov 11;116(19):3875-86. PMID: 20631379

“B cells were identified by staining with PE-conjugated anti-CD19 (clone 1D3) antibody. Flow cytometry analysis showed eradication of CD19+ cells in lymphoma. The antibody 1D3 was used to gate B cell population in bone marrow. Peripheral blood B cells were monitored weekly using 1D3. CAR-T cells specifically targeted CD19+ cells as confirmed by flow.”

4. Self-renewal of B-1 lymphocytes is dependent on CD19.

Krop I, et al. Eur J Immunol. 1996 Jan;26(1):238-42. PMID: 8566073

“Cells were stained with anti-CD19 mAb (1D3) for flow sorting. Peritoneal cells were analyzed for CD19 expression using 1D3. The antibody 1D3 was used in all flow cytometry experiments for B cell identification. Adoptive transfer experiments used sorted CD19+ cells with 1D3. B-1 cell self-renewal was assessed by limiting dilution analysis after sorting with 1D3.”

5. The signaling activity of murine CD19 is regulated during cell development.

Krop I, et al. J Immunol. 1996 Jul 1;157(1):48-56. PMID: 8683154

“Murine B cells were stained with biotinylated 1D3 anti-CD19 mAb followed by streptavidin-PE. Flow cytometry was used to assess CD19 expression levels during development. The 1D3 clone was specific for murine CD19. Developmental stages were gated based on 1D3 staining intensity. Signaling was correlated with CD19 surface density measured by 1D3.”

Ship from Country	USA
Size	1 mg Clear

Anti-human CD19 scFv (FMC63)

Anti-human CD19 scFv (FMC63)