Extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells.
Hybridoma antibody sequencing service(hybridoma sequencing, monoclonal antibody cloning, hybridoma cloning): all isotypes (IgG, IgM); antibody variable regions from mouse, rat, armenian hamster, rabbit, human; hybridomas or clonal B cells; sequenced after PCR amplification and DNA...
hybridoma-based custom mouse monoclonal antibody development services: production of anti-peptide, protein, chemical, cell monoclonal antibodies; DNA immunization; RIMMS; monoclonal antibodies for ELISA, Western blot, IHC, flow cytometry, diagnostics and therapeutics.
Kinetic analysis is used to determine the affinity of an antibody to its soluble antigen using surface plasmon resonance (SPR) or biolayer interferometry (BLI), which can measure kon, koff, and kD. Association and dissociation are...
to obtain the intact amino acid sequence of variable and/or constant regions of monoclonal antibodies by LC-MS/MS.
Fast and high yield stable cell line development services for biologics production: generation of stable cell lines expressing recombinant proteins and antibodies at high yield. Production yield of recombinant antibodies can be >2 g/liter.
Antibody optimization service (antibody affinity maturation service) uses molecular evolution and high throughput screening to improve antibody potency and to save cost of antibody production.
developed human monoclonal antibodies and monkey monoclonal antibodies using antigen specific single B cell-based antibody generation technologies. After detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, the...
Antibody humanization service using empirical and rational approaches. Humanized antibodies are designed by our proprietary humanization and optimization algorithm, and made by gene synthesis and fusion with human antibody backbones.
single B cell antibody discovery technologies for antigen specific single B cell antibody sequencing after flow cytometry-based single B cell sorting. rabbit memory B cell culture, mouse plasma cell antibody cloning.
