[KO Validated] MSH2 Rabbit mAb (A22177)

Catalog No.	A22177
Product Name	[KO Validated] MSH2 Rabbit mAb (A22177)
Supplier Name	ABclonal, Inc.
Brand Name	Abclonal
Synonyms	FCC1; COCA1; HNPCC; LCFS2; MSH-2; hMSH2; HNPCC1; LYNCH1; MMRCS2
Gene Name	MSH2
Protein Name	MSH2
Uniprot/Swissprot ID	P43246
Entrez GeneID	4436
Clone	ARC55799
Clonality	Monoclonal
Source/Host	Rabbit
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Conjugate	Unconjugated
Note	Products will be shipped from the warehouse in Massachusetts. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com.
Order Offline	Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com

Description

A22177: [KO Validated] MSH2 Rabbit mAb

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information about [KO Validated] MSH2 Rabbit mAb (A22177)

Immunogen：Recombinant fusion protein containing a sequence corresponding to amino acids 330-583 of human MSH2 (NP_000242.1).
Sequence：LNKCKTPQGQRLVNQWIKQPLMDKNRIEERLNLVEAFVEDAELRQTLQEDLLRRFPDLNRLAKKFQRQAANLQDCYRLYQGINQLPNVIQALEKHEGKHQKLLLAVFVTPLTDLRSDFSKFQEMIETTLDMDQVENHEFLVKPSFDPNLSELREIMNDLEKKMQSTLISAARDLGLDPGKQIKLDSSAQFGYYFRVTCKEEKVLRNNKNFSTVDIQKNGVKFTNSKLTSLNEEYTKNKTEYEEAQDAIVKEIVN
Gene ID：4436
Swiss prot：P43246
Synonyms：FCC1; COCA1; HNPCC; LCFS2; MSH-2; hMSH2; HNPCC1; LYNCH1; MMRCS2; H2
Calculated MW：105kDa
Observed MW：105kDa

Images of [KO Validated] MSH2 Rabbit mAb (A22177)

Western blot analysis of extracts from wild type(WT) and MSH2 knockout (KO) HeLa cells, using MSH2 antibody (A22177) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of various lysates, using MSH2 antibody (A22177) at1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using [KO Validated] MSH2 Rabbit mAb (A22177) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human tonsil using [KO Validated] MSH2 Rabbit mAb (A22177) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Please remember our product information: [KO Validated] MSH2 Rabbit mAb (Catalog Number: A22177) Abclonal

Additional information

Ship from Country	USA
Size	20 ul, 100 ul, 200 ul, 50 ul

No more offers for this product!