Biotinylated Anti-mouse MHC Class II (I-A/I-E) Antibody (M5/114.15.2, Human IgG1 Fc Silent Kappa)
$90.00 – $180.00
Recombinant anti-mouse MHC Class II (I-A/I-E) monoclonal antibody (Clone M5/114.15.2, Biotinylated), engineered with a Human IgG1 Fc-Silent domain to minimize non-specific Fc receptor binding during flow cytometry and antigen presentation assays. Variable regions are derived from the classic rat anti-mouse MHC Class II clone M5/114.15.2 and recombinantly expressed in mammalian cells.
- Form & Conjugation: Biotinylated, Flow Cytometry Grade
- Applications: Flow Cytometry (FC), Functional Assays
- Alternative Formats: Non-biotinylated FC grade, unconjugated in vivo grade, and recombinant rat IgG2b versions are also available.
- Details & Specifications
- References
| Catalog No. | PA007795.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibody (Clone: M5/114.15.2), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | M5/114.15.2 |
| Isotype | Human IgG1 Fc Silent Kappa |
| Source/Host | The site-directed biotinylated anti-mouse MHC Class II (I-A/I-E) monoclonal antibody (clone: M5/114.15.2) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant M5/114.15.2 antibody specifically binds to mouse MHC Class II (I-A/I-E). |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The recombinant anti-mouse MHC Class II (I-A/I-E) monoclonal antibody of clone M5/114.15.2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007795.h1Fs-b: Biotinylated Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibody (Clone: M5/114.15.2), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
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