Biotinylated Anti-mouse CD8a Antibody (YTS 105.18, Human IgG1 Fc Silent Kappa)

Catalog No.	PA007728.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: YTS 105.18), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	YTS 105.18
Isotype	Human IgG1 Fc Silent Kappa.
Source/Host	The site-directed biotinylated anti-mouse CD8a monoclonal antibody (clone: YTS 105.18) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant YTS 105.18 antibody specifically binds to the mouse CD8a protein.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC
Shipping	The YTS 105.18 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007728.h1Fs-b: Biotinylated Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: YTS 105.18), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

The rat anti-mouse CD8a monoclonal antibody clone YTS 105.18 specifically reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene. While some alternative suppliers and historical researchers mistakenly misidentify the YTS 105.18 clone as a rat IgG2a antibody, heavy chain variable region sequence validation strictly confirms that its true biological isotype is rat IgG2b kappa. In immunological research, the CD8 protein is a critical marker for cell-mediated immune defense and T-cell development, serving as a pivotal diagnostic and prognostic indicator in inflammatory disorder and tumor profiling datasets.

Syd Labs’ recombinant anti-mouse CD8a antibody for flow cytometry (clone YTS 105.18) is engineered to deliver exceptional batch-to-batch consistency and high signal-to-noise ratios. Because clone YTS 105.18 functionally acts as a non-depleting anti-mouse CD8a reagent?blocking receptor interactions without triggering systemic cell death?it represents the gold-standard framework for Flow Cytometry (FC/FACS) applications that require viable cell surface staining and artifact-free FACS sorting. Available in multiple highly purified formats, including native rat IgG2b, advanced human IgG1 fc silent engineered variants to completely eradicate non-specific Fc-receptor binding, and biotinylated anti-mouse cd8 clone yts 105.18 conjugates, this product pipeline is meticulously optimized to integrate seamlessly into complex multi-color flow cytometry panels.

Frequently Asked Questions (FAQ) about Recombinant Anti-Mouse CD8a Antibody (Clone: YTS 105.18) for Flow Cytometry:

Q: What is the difference between anti-mouse CD8a clones YTS 105.18 and 2.43 for flow cytometry?
While both clones target the mouse T-cell surface glycoprotein CD8 alpha chain, they serve completely distinct experimental purposes. Clone 2.43 is primarily a depleting antibody widely utilized for in vivo T-cell clearance. Conversely, clone YTS 105.18 is functionally non-depleting. For flow cytometry (FC/FACS) and live-cell sorting, Syd Labs’ recombinant clone YTS 105.18 is the optimal choice because it binds and stains CD8a+ cells efficiently without triggering systemic cell clearance or antibody-dependent cellular cytotoxicity (ADCC), ensuring high cell viability post-sorting.

Q: How does a recombinant Fc silent anti-mouse CD8a antibody improve FACS results?
Traditional hybridoma-derived antibodies often exhibit non-specific binding to Fcγ receptors (FcγR) expressed on immune cells (such as macrophages, B cells, and NK cells), leading to false-positive signals and high background fluorescence in multi-color flow cytometry. Syd Labs’ recombinant human IgG1 Fc silent engineered framework incorporates specific mutations within the Fc domain to completely abolish its binding affinity to Fc receptors. This engineered Fc silent anti-mouse CD8 antibody eliminates the need for extensive Fc-blocking steps, significantly enhancing the signal-to-noise ratio and delivering precise gating for sensitive multicolor flow panels.

Q: What are the advantages of using Biotinylated or Avi-tag Recombinant Anti-Mouse CD8a clones?
Using a biotinylated anti-mouse CD8 clone YTS 105.18 antibody provides a highly sensitive, flexible detection method when paired with fluorochrome-conjugated streptavidin in multi-color flow panels. Syd Labs’ biotin avi tag formats utilize site-specific, enzymatically controlled biotinylation during recombinant production. Unlike harsh chemical biotinylation which randomly targets lysine residues and can compromise the antibody’s antigen-binding site, the Avi-tag ensures that every single antibody molecule is uniformly biotinylated at a unique peptide sequence. This preserves maximum binding affinity and yields exceptional batch-to-batch consistency in flow cytometric staining.

Q: Is the anti-mouse CD8a antibody clone YTS 105.18 a rat IgG2a or rat IgG2b isotype?
There is widespread misinformation in some supplier catalogs and older literature stating that clone YTS 105.18 is a rat IgG2a. However, precise heavy chain variable region sequence validation confirms that the true biological isotype of clone YTS 105.18 is rat IgG2b kappa. Syd Labs strictly adheres to sequence-verified recombinant production standards, ensuring that our native formats match the authentic rat IgG2b sequence, allowing you to select the absolute correct isotype control antibody for your flow cytometry controls.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD8 monoclonal antibodies:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 53-6.72)
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7)
Recombinant Anti-mouse CD8 (Lyt 2.1) monoclonal antibody (Clone: 116-13.1)

Syd Labs provides the following in vivo grade recombinant anti-human CD8 monoclonal antibodies:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8)

Syd Labs provides the following recombinant anti-mouse CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18) for flow cytometry
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7) for flow cytometry

Syd Labs provides the following recombinant anti-human CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8) for flow cytometry