Biotinylated Anti-mouse CD8a Antibody (2.43, Human IgG1 Fc Silent Kappa)

Catalog No.	PA007726.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: 2.43), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	2.43
Isotype	Human IgG1 Fc Silent Kappa.
Source/Host	The site-directed biotinylated anti-mouse CD8a monoclonal antibody (clone: 2.43) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant 2.43 antibody specifically binds to mouse CD8a protein.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC
Shipping	The 2.43 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007726.h1Fs-b: Biotinylated Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: 2.43), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

The rat anti-mouse CD8a monoclonal antibody 2.43 (rat IgG2b kappa) reacts with the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) encoded by the mouse CD8A gene that encodes the CD8a chain of the dimeric CD8 protein. The mouse CD8 protein is primarily responsible for cell-mediated immune defense and T-cell development. CD8A has been widely reported as a potential prognosis and diagnostic marker for several diseases, such as inflammatory disorders and tumors. It was shown that the anti-mouse CD8a rat monoclonal antibody (clone number: 2.43) can be used for depleting activity in vivo.
Our recombinant 2.43 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD8a monoclonal antibody (hybridoma clone name or number: 2.43).

References of Anti-Mouse CD8a Monoclonal Antibody (Clone: 2.43):

1. Macrophage Migration Inhibitory Factor protects cancer cells from immunogenic cell death and impairs anti-tumor immune responses

Balogh, K. N., et al. PLoS One. 2018 Jun 4;13(6):e0197702. PMID: 29864117

“Beginning two days before 4T1 cell tumor implantation, mice were treated with intraperitoneal injection of anti-CD4 (clone GK1.5) and anti-CD8 (clone 2.43) antibodies in PBS to evaluate their effects on anti-tumor immune responses.”

2. Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8+ T Cells

Li, J., et al. Immunity. 2018 Apr 17;48(4):773-786.e5. PMID: 29625896

“To deplete CD4+ or CD8+ T cells, 100 μg of anti-CD4 (GK 1.5) or anti-CD8 (2.43) was injected i.p. on Day -1, 2, 7, 12 and 17 in mouse tumor models.”

3. Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses

Moynihan, K. D., et al. Nat Med. 2016 Dec;22(12):1402-1410. PMID: 27775706

“For T cell depletion, 200 μg of anti-CD8α (clone 2.43), anti-CD4 (clone GK1.5), or anti-NK1.1 (clone PK136) were administered i.p. every 3-4 d starting 1 d before treatment.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD8a Monoclonal Antibody (Clone: 2.43) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD8 monoclonal antibodies:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18)
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 53-6.72)
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7)
Recombinant Anti-mouse CD8 (Lyt 2.1) monoclonal antibody (Clone: 116-13.1)

Syd Labs provides the following in vivo grade recombinant anti-human CD8 monoclonal antibodies:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8)

Syd Labs provides the following recombinant anti-mouse CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: 2.43) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 169.4) for flow cytometry
Recombinant Anti-mouse CD8a monoclonal antibody (Clone: YTS 105.18) for flow cytometry
Recombinant Anti-mouse CD8b monoclonal antibody (Clone: YTS 156.7) for flow cytometry

Syd Labs provides the following recombinant anti-human CD8 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD8a monoclonal antibody (Clone: OKT8) for flow cytometry