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Biotinylated Anti-mouse CD3 Antibody (17A2, Human IgG1 Fc Silent Kappa)

Name: Biotinylated Anti-mouse CD3 Antibody (17A2, Human IgG1 Fc Silent Kappa)
Brand: Syd Labs
SKU: PA007255.h1Fs-b
Availability: InStock

Catalog No.: PA007255.h1Fs-b | Isotype: Human IgG1 Fc Silent Kappa | Flow Cytometry

$45.00 – $90.00

Recombinant biotinylated anti-mouse CD3 monoclonal antibody (Clone 17A2, SKU PA007255.h1Fs-b), engineered with a Human IgG1 D265A (Fc-Silent) domain to eliminate Fc gamma receptor-mediated effects. Variable regions are derived from the classic rat anti-mouse CD3 clone (17A2) and recombinantly expressed in mammalian cells.

Endotoxin Level: < 1.0 EU/mg (Ultra-low, In Vivo Grade)
Applications: Flow Cytometry (FC), T Cell Stimulation, In Vivo Blocking
Alternative Formats: Unconjugated, alternative Fc-silent backbones, and fluorescently labeled versions of clone 17A2 are also available.

Details & Specifications
References

Catalog No.	PA007255.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	17A2
Isotype	Human IgG1 Fc Silent Kappa
Source/Host	The site-directed biotinylated anti-mouse CD3 monoclonal antibody (clone: 17A2) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant 17A2 antibody specifically binds to the mouse T cell receptor CD3.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The 17A2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007255.h1Fs-b: Biotinylated Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

The 17A2 antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes.

References of Anti-Mouse CD3 Monoclonal Antibody (Clone: 17A2)：

1. Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody

Sugita S, Shimizu J, Makabe K, Keino H, et al. Arthritis Res Ther. 2017 Jul 25;19(1):176. PMID: 28743289

“In brief, CFSE-labeled C57BL/6JJcl splenocytes (2 × 10^6 cells/well) and irradiated (20 Gy) BALB/c splenocytes (2 × 10^5 cells/well) were cocultured in 24-well plates with 1 ?g/ml of Dow2, a control anti-mouse CD3 ? Ab (clone 17A2), or an isotype control (rat IgG)…Mouse pan-T cells isolated from C57BL/6JJcl donors were incubated with 1 ?g/ml of Dow2 or a control anti-mouse CD3 Ab (clone 17A2 or clone 145-2C11) at 4 °C for 30 min…Incubation with Dow2 alone or rat IgG did not stimulate T cells to produce detectable IFN-? (Fig. 2b, left panel) or IL-17 (Fig. 2c, left panel). In contrast, T cells incubated with 17A2 (anti-mouse CD3 antibody) produced increased levels of cytokines which were significantly reduced in T cells incubated with both Dow2 and 17A2 (anti-mouse CD3 mAb)…In contrast, IRBP-stimulated production of IFN-? was significantly enhanced in ocular cells incubated with 17A2 antibody (P < 0.005)…The expression of CD4 and CD8 on splenocytes from normal control mice and Dow2- or rat IgG-treated EAU mice was assessed by flow cytometry.”

2. In vivo Imaging Technologies to Monitor the Immune System

McCarthy CE, White JM, Viola NT, Gibson HM. Front Immunol. 2020 Jun 2;11:1067. PMID: 32582173

“Upon ex vivo analysis, one study reported a relative depletion of CD4 T cells and an expansion of CD8 T cells after delivery of an 89Zr-anti-CD3 tracer (clone 17A2) in mice…Conjugation of the contrast material to an antibody allows for specific targeting of a cell population or protein of interest…Protein platforms including antibodies, cytokines, and receptor ligands are also popular choices as molecular imaging agents for positron emission tomography (PET), single-photon emission computerized tomography (SPECT), scintigraphy, and optical imaging…Promising new adoptive cell therapies (ACT) for cancer, including ex vivo expanded tumor-infiltrating lymphocytes (TILs) and chimeric antigen receptor T cells (CAR-T cells), would benefit from imaging technologies that track cell fate in vivo…CD8 + cytotoxic T lymphocytes (CTLs) specific to the melanoma antigen Melan-A were 111In-labeled, infused in patients, and evaluated using serial whole body and static gamma camera imaging.”

3. Quantitative CD3 PET Imaging Predicts Tumor Growth Response to Anti-CTLA-4 Therapy

Larimer BM, Wehrenberg-Klee E, Caraballo A, Mahmood U. J Nucl Med. 2016 Oct;57(10):1607-1611. PMID: 27230929

“The monoclonal antimouse CD3 antibody clone 17A2 was purchased from…Conjugation and radiolabeling of the CD3 antibody resulted in a pure, highly specific probe for CD3-expressing cells…For Western blotting, the tumors were lysed in 1% sodium dodecyl sulfate and subjected to sodium dodecyl sulfate?polyacrylamide gel electrophoresis…Blots were transferred to nitrocellulose, blocked with 5% nonfat dry milk, and probed with the appropriate antibodies…Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded 10-?m slices of tumor to confirm the cellular location of CD3 expression.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD3 Monoclonal Antibody (Clone: 17A2) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant anti-mouse CD3e monoclonal antibodies for flow cytometry:

Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
Biotin Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
FITC Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
APC Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
PE Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
Pacific Blue Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
PerCP Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
PE/Cy5 Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
PE/Cy7 Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
iFluor 488 Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
iFluor 549 Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
iFluor 594 Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
iFluor 647 Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
iFluor 700 Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
APC/Cy7 Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)
PerCP/Cy5.5 Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11)