Biotinylated Anti-mouse CD25 Antibody (PC61.5.3, Human IgG1 Fc Silent Kappa)
$65.00 – $130.00
Recombinant anti-mouse CD25 / IL-2Rα monoclonal antibody (Clone PC61.5.3, Biotinylated), engineered with a Human IgG1 Fc-Silent domain to minimize non-specific Fc receptor binding during regulatory T cell (Treg) detection and flow cytometry assays. Variable regions are derived from the classic rat anti-mouse CD25 clone PC61.5.3 and recombinantly expressed in mammalian cells.
- Form & Conjugation: Biotinylated, Flow Cytometry Grade
- Applications: Flow Cytometry (FC), Regulatory T Cell (Treg) Analysis, Functional Assays
- Alternative Formats: Non-biotinylated FC grade, unconjugated in vivo depletion grade, rat IgG1 versions, and Syd Labs daclizumab / anti-human CD25 antibodies are also available.
- Details & Specifications
- References
| Catalog No. | PA007718.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-mouse CD25 Monoclonal Antibody (Clone: PC61.5.3), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | PC61.5.3. |
| Isotype | Human IgG1 Fc Silent Kappa |
| Source/Host | The site-directed biotinylated anti-mouse CD25 monoclonal antibody (clone: PC61.5.3) was produced in mammalian cells |
| Specificity/Sensitivity | The site-directed biotinylated recombinant PC61.5.3 antibody specifically binds to mouse CD25 protein |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC |
| Shipping | The PC61.5.3 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant rat IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. Recombinant anti-mouse CD25 monoclonal antibodies (clone: PC61, PC61.5 or PC61.5.3) produced from CHO or engineered CHO cell lines good for in vitro and in vivo studies. The afucosylated antibody may behave better in in vivo regulatory T cell depletion. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007718.h1Fs-b: Biotinylated Recombinant Anti-mouse CD25 Monoclonal Antibody (Clone: PC61.5.3), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
The in vivo grade recombinant anti-mouse CD25 monoclonal antibodies are based on the rat monoclonal antibody anti-CD25 (mouse) with the clone number of PC61, PC61.5 or PC61.5.3. The rat IgG1 lamda monoclonal antibody has been validated and reported for use in immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. In vivo depletion of mouse CD25(+)CD4(+)FoxP3(+) Treg cells using the rat PC61 monoclonal antibody is widely used to characterize the Treg function. The PC61 monoclonal antibody specifically binds to an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by the 3C7 and 7D4 monoclonal antibodies. By doing so, the antibody blocks binding of IL-2 to CD25.
Related Anti-Mouse CD25 Recombinant Monoclonal Antibodies (In Vivo Grade):
Recombinant Anti-mouse CD25 monoclonal antibodies (Clone: PC61.5.3)
Related Anti-Human CD25 / CD25-Targeting Recombinant Monoclonal Antibodies (In Vivo Grade):
Recombinant Anti-mouse CD25 (IL-2Rα) monoclonal antibodies (Clone: 7G7B6)
Related Research Grade Antibody Biosimilar Proteins:
Daclizumab Biosimilar, research grade, Anti-human CD25 Monoclonal Antibody
Related Anti-Mouse CD25 Recombinant Monoclonal Antibodies for Flow Cytometry:
Recombinant Anti-mouse CD25 monoclonal antibodies (Clone: PC61.5.3) for flow cytometry
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