Anti-mouse CD25 Monoclonal Antibody (Clone: PC61.5.3) | PA007489.h1Fs

Catalog No.	PA007489.h1Fs
Product Name	Anti-mouse CD25 Monoclonal Antibody (Clone: PC61.5.3) | PA007489.h1Fs
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	IL-2Rα, Ly-43, p55, Tac
Clone	PC61.5.3.
Isotype	Human IgG1 kappa.
Source/Host	The anti-mouse CD25 monoclonal antibody (clone: PC61.5.3) was produced in mammalian cells.
Specificity/Sensitivity	The recombinant PC61.5.3 antibody specifically binds to mouse CD25 protein.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-mouse CD25 Monoclonal Antibody (Clone: PC61.5.3), Human IgG1 Fc Silent Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant anti-mouse CD25 monoclonal antibodies (clone: PC61, PC61.5 or PC61.5.3) produced from CHO or engineered CHO cell lines good for in vitro and in vivo studies. The afucosylated antibody may behave better in in vivo regulatory T cell depletion.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007489.h1Fs: Recombinant Anti-mouse CD25 Monoclonal Antibody (Clone: PC61.5.3), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.

Background

The in vivo grade recombinant anti-mouse CD25 monoclonal antibodies are based on the rat monoclonal antibody anti-CD25 (mouse) with the clone number of PC61, PC61.5 or PC61.5.3. The rat IgG1 lamda monoclonal antibody has been validated and reported for use in immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells. In vivo depletion of mouse CD25(+)CD4(+)FoxP3(+) Treg cells using the rat PC61 monoclonal antibody is widely used to characterize the Treg function. The PC61 monoclonal antibody specifically binds to an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by the 3C7 and 7D4 monoclonal antibodies. By doing so, the antibody blocks binding of IL-2 to CD25.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD25 monoclonal antibodies:

Recombinant Anti-mouse CD25 monoclonal antibodies (Clone: PC61.5.3)

Syd Labs also provides the following research grade antibody biosimilar proteins:

Daclizumab Biosimilar, research grade, Anti-human CD25 Monoclonal Antibody

Recombinant Anti-mouse CD25 Monoclonal Antibody (Clone: PC61.5.3), Human IgG1 Fc Silent Kappa from: Recombinant Anti-mouse CD25 Monoclonal Antibody (Clone: PC61.5.3), Human IgG1 Fc Silent Kappa: PA007489.h1Fs Syd Labs

No more offers for this product!