Catalog No.	PA007712.m2a
Product Name	Anti-mouse V beta 8 TCR Monoclonal Antibody (Clone: F23.1) | PA007712.m2a
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	TCR V beta 8; TCR V beta 8.1/8.2/8.3
Clone	F23.1.
Isotype	Mouse IgG2a kappa.
Source/Host	The anti-mouse V beta 8 TCR monoclonal antibody (clone: F23.1) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: F23.1) specifically binds to mouse V beta 8 TCR.
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse V beta 8 TCR protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-mouse V beta 8 TCR monoclonal antibody of clone F23.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007712.m2a: In Vivo Grade Recombinant Anti-mouse V beta 8 TCR Monoclonal Antibody (Clone: F23.1), Mouse IgG2a Kappa

Recombinant mouse IgG2a monoclonal antibody.

References of Anti-mouse V beta 8 TCR Monoclonal Antibody:

Antigen receptor-mediated depletion of FOXP3 in induced regulatory T-lymphocytes via PTPN2 and FOXO1.
Bothur, E., et al. Nat Commun. 2015 Oct 13. doi: 10.1038/ncomms9576. PMID: 26815406
The superantigen SEB is recognized by all TCRs carrying a V? gene of the V?8 family, but not by V?6 family members. …As shown in Fig. 1f, V?8+ and V?6+ iTregs kept high levels of FOXP3 after re-culture without SEB, while in its presence V?8+ but not V?6+ iTregs downregulated FOXP3 to a great extent. …Cells re-stimulated by SEB (see above) were first incubated for 5?min with the ?CD16/32 FcR blocking antibody (2.4G2; BD, 5??g?ml?1), followed by extracellular staining using either ?V?6 (50% of SN of 44-22-1 cells) plus ?-rat-IgG-FITC or ?V?8 (F23.1; ascites, 1:100) plus ?-mouse-IgG-Alexa488 (Life Technologies, 1:1,000), or the respective isotype controls. …As explained before, GFP positivity of these cells reflects active transcription of foxp3. …Together, these results from both types of reporter cells clearly indicate suppressed gene transcription/translation as the reason for downregulation of FOXP3 protein.
Tags: activity of Anti-Mouse V beta 8 TCR Monoclonal; Anti-Mouse V beta 8 TCR Monoclonal

Staphylococcal enterotoxin B modulates V beta 8+ TcR-associated T-cell memory against conventional antigen.
Bell, S. J., et al. Cell Immunol. 1995 Jan;160(1):58-64. doi: 10.1016/0008-8749(95)80009-8. PMID: 7842486
Primary in vivo challenge with the superantigen staphylococcal enterotoxin B (SEB) induces polyclonal proliferation of an unusually large proportion of circulating T-cells that bear the V?8?T-cell receptor (TcR) domain. …Recently we demonstrated that more cells than just V?8+ T-cells undergo clonal proliferation after challenge with SEB (Cell. Immunol. 154, 440, 1994). …These findings suggested that non-V?8+ T-cells may have a role in the induction of superantigen-induced anergy. …Using these kinetic data we investigated whether challenge with SEB would modulate antigen-specific V?8-associated T-memory responses. …Results indicated that challenge of SWM-primed mice with SEB abrogated the V?8-associated SWM-specific T-cell memory for an extended but transient period of time.
Tags: Anti-Mouse V beta 8 TCR Monoclonal antibody for animal model; Anti-Mouse V beta 8 TCR Monoclonal antibody for flow cytometry

In vivo preferential usage of TCR V beta 8 in Torpedo acetylcholine receptor immune response in the murine experimental model of myasthenia gravis.
Aimé-Sempé, C., et al. J Neuroimmunol. 1995 May;58(2):191-200. doi: 10.1016/0165-5728(95)00017-v. PMID: 7759608
The specific proliferation towards AChR was found to be blocked by anti-V?8.1,2,3 and to a lesser extent by anti-V?5 mAbs, but not by the other antibodies used (anti-V?2, V?6, V?9). …Involvement of V?8 subfamilies was also explored in vivo. …After 7 days of treatment, there was a striking inhibition of the proliferative response of cells from anti-V?8.1,2,3-treated mice and a moderate inhibition when using anti-V?.1,2 and anti-V?8.2 antibodies. …Monoclonal antibody F23.1 against T cell bearing V?8 TCR elements can prevent the development of insulitis in NOD mice. …Monoclonal antibody F23.1 against T cell bearing V?8 TCR elements can prevent the development of insulitis in NOD mice.
Tags: Anti-Mouse V beta 8 TCR Monoclonal antibody in vivo; Anti-Mouse V beta 8 TCR Monoclonal for mouse tumor model

Cross-talk between V beta 8+ and gamma delta+ T lymphocytes in contact sensitivity.
Dieli, F., et al. Immunology. 1998 Apr;93(4):469-77. doi: 10.1046/j.1365-2567.1998.00435.x. PMID: 9659217
Analysis of the role played by V?8+ T lymphocytes during in vivo responses to TNCB, have been undertaken using TNCB-specific T-cell lines. …Against this background, we decided to investigate the role played by V?8+ T lymphocytes in two examples of immune response to TNCB in vivo, namely the CS reaction and its passive transfer. …To confirm the requirement for V?8+ lymphocytes in the CS reaction to TNCB, passive transfer experiments were undertaken. …It may be concluded that V?8+ lymphocytes play a pivotal role in the CS reaction to TNCB. …To ascertain which cell within the V?8? population was important in the passive transfer of the CS reaction, the V?8? population was further depleted of ??+ or ??+ cells and cotransferred together with V?8+ cells into naive recipient mice.
Tags: function of Anti-Mouse V beta 8 TCR Monoclonal; rat Anti-Mouse V beta 8 TCR Monoclonal

For more references about Anti-mouse V beta 8 TCR Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse V beta 8 TCR Monoclonal Antibody (Clone: F23.1), Mouse IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse V beta 8 TCR Monoclonal Antibody (Clone: F23.1), Mouse IgG2a Kappa: PA007712.m2a Syd Labs

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