Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2) | PA007711.m1

Catalog No.	PA007711.m1
Product Name	In Vivo Grade Recombinant Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2), Mouse IgG1 Kappa
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	T cell receptor
Clone	1B2.
Isotype	Mouse IgG1 kappa.
Source/Host	The anti-mouse 2C TCR monoclonal antibody (clone: 1B2) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: 1B2) specifically binds to mouse 2C TCR .
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse 2C TCR protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-mouse 2C TCR monoclonal antibody of clone 1B2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007711.m1: In Vivo Grade Recombinant Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2), Mouse IgG1 Kappa

Recombinant mouse IgG1 monoclonal antibody.

References of Anti-mouse 2C TCR Monoclonal Antibody

1. STING-dependent cytosolic DNA sensing mediates innate immune recognition of immunogenic tumors.
Woo, S. R., et al.Immunity. 2014 Nov 20;41(5):830-42. PMID: 25517615
“Because MyD88 can function in a T cell-intrinsic fashion, we performed adoptive transfer of wild-type (WT) CFSE-labeled 2C TCR Tg T cells (specific for the model antigen SIY) into WT or Myd88-/- mice and challenged with B16.SIY tumors. To evaluate the mechanism by which the absence of host STING resulted in impaired antitumor T cell responses, we adoptively transferred CFSE-labeled 2C TCR Tg T cells into WT and STING-deficient mice and measured T cell proliferation. Splenocytes from recipient mice were analyzed after staining with anti-mouse CD8α-APC and biotin-labeled anti-2C-TCR (1B2) with SA-PE by flow cytometry to assess CFSE dilution.”

2. Murine anti-third-party central-memory CD8(+) T cells promote hematopoietic chimerism under mild conditioning: lymph-node sequestration and deletion of anti-donor T cells.
Ophir, E., et al.Blood. 2013 Feb 14;121(7):1220-8. PMID: 23223359
“Stained 2C CD8+ HTCs and CB6 Tcms (recognized specifically by the 2C TCR transgene) were adoptively transferred into lethally and sublethally irradiated C57BL/6 mice. The number of 2C TCR Tg cells used is within the physiologic range observed for naive antigen-specific precursors. In addition, the 2C-derived CD8+ cells can be identified using the clonotypic 1B2 antibody directed against their transgenic TCR.”

3. KRAS G12V neoantigen specific T cell receptor for adoptive T cell therapy against tumors.
Lu, D., et al.Nat Commun. 2023 Oct 12;14(1):6389. PMID: 37828002
“1-2C TCR-T cells show tumor suppression efficacy in a tumor-bearing mouse model and improved inhibition of tumor growth in combination with anti-PD-1 antibodies. Of note, 1-2C TCR is a public TCR that presents in each of the four mice and dominated KRAS-G12V-9 specific TCRs. The 1-2C TCR has higher binding affinity and immune responsiveness against tumor cells than the 3-2E TCR, and 1-2C was thus selected for further in vivo anti-tumor evaluations.”

4. Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Nishimura, H., et al.Immunity. 1999 Aug;11(2):141-51. PMID: 10485649
“Anti-2C TCRαβ clonotypic mAb, 1B2, and anti-mouse PD-1 mAb (J43) were biotinylated. For immunochemistry, cryosections were pretreated with 2% BSA in PBS/0.05% Tween and incubated with biotinylated CD8α or 1B2 overnight. Unlike most of the lupus-prone mice, common autoantibodies represented by anti-double strand DNA (dsDNA) and rheumatoid factor (RF) were hardly detected in B6-PD-1-/- mice.”

5. Structure-Based, Rational Design of T Cell Receptors.
Zoete, V., et al.Front Immunol. 2013 Sep 12;4:268. PMID: 24062738
“Using the well-characterized 2C TCR/SIYR/H-2Kb structure as a model system, we demonstrated that a binding free energy decomposition based on the MM-GBSA approach provides a detailed and reliable description of the TCR/pMHC interactions. To assess the ability of the MM-GBSA approach to identify quantitatively the hot-spot residues for the TCR/pMHC association, we performed a study of the 2C TCR/SIYR/H-2Kb system using both the CAS and BFED methods. Our results also showed that BFED provided a more detailed and reliable description of the interactions between the TCR and pMHC molecules when including entropic terms.”

In Vivo Grade Recombinant Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2), Mouse IgG1 Kappa from: In Vivo Grade Recombinant Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2), Mouse IgG1 Kappa: PA007711.m1 Syd Labs