Anti-Mouse / Human CD44 Antibody (Clone:IM7.8.1) | PA007622.r2b

Catalog No.	PA007622.r2b
Product Name	In Vivo Grade Recombinant Anti-mouse/human CD44 Monoclonal Antibody (Clone: IM7.8.1), Rat IgG2b Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	CD44 antigen precursor; CDw44, ECMR-III, Epican, Extracellular matrix receptor-III
Clone	IM7.8.1.
Isotype	Rat IgG2b kappa.
Source/Host	The anti-mouse/human CD44 monoclonal antibody (clone: IM7.8.1) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant rat monoclonal antibody (clone: IM7.8.1) specifically binds to mouse/human CD44.
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse/human CD44 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-mouse/human CD44 monoclonal antibody of clone IM7.8.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007622.r2b: In Vivo Grade Recombinant Anti-mouse/human CD44 Monoclonal Antibody (Clone: IM7.8.1), Rat IgG2b Kappa

Recombinant rat IgG2b Monoclonal Antibody.

References of Anti-Mouse CD44 Monoclonal Antibody (Clone: IM7.8.1)

1. CD44-mediated hematopoietic progenitor cell adhesion and its complex role in myelopoiesis.
Andrés Hidalgo, et al.J Hematother Stem Cell Res. 2002 Jun;11(3):539-47. doi: 10.1089/15258160260091004. PMID: 12183839
“Flow cytometry and immunodepletion analysis indicated that two antibodies, MA-8 and MA-2, recognized CD44. Although MA-8 and another anti-CD44 antibody, IM7.8.1, blocked adhesion of hematopoietic cells to hyaluronate, adhesion to BM stroma was notably increased by these mAb, suggesting minor roles for CD44/hyaluronate interaction and the triggering of additional adhesion pathways upon CD44 engagement….”

2. CD44 involvement in experimental collagen-induced arthritis (CIA).
Nedvetzki, S., et al.J Autoimmun. 1999 Aug;13(1):39-47. doi: 10.1006/jaut.1999.0294. PMID: 10441166
“Even accelerated disease developed after two injections of type II collagen was markedly inhibited by IM7.8.1 anti-CD44 mAb. Although KM81 anti-CD44 mAb is a less efficient anti-arthritogenic reagent than IM7.8.1, its Fab’ fragments partially inhibit CIA…”

3. CD44 and hyaluronic acid regulate in vivo iNOS expression and metalloproteinase activity in murine air-pouch inflammation.
Cabrera, P. V., et al.Inflamm Res. 2004 Oct;53(10):556-66. doi: 10.1007/s00011-004-1295-8. PMID: 15597151
“To evaluate the effects of anti-CD44 IM7.8.1 antibody, HMW-HA and LMW-HA on leukocyte migration and adhesion, and the induction of proinflammatory mediators, in mouse air-pouch inflammation induced by zymosan…”

4. Ras, protein kinase C zeta, and I kappa B kinases 1 and 2 are downstream effectors of CD44 during the activation of NF-kappa B by hyaluronic acid fragments in T-24 carcinoma cells.
Fitzgerald, K. A., et al.J Immunol. 2000 Feb 15;164(4):2053-63. doi: 10.4049/jimmunol.164.4.2053. PMID: 10657658
“The effect of HA was size dependent as neither disaccharide nor native HA were active. CD44, the principal cellular receptor for HA, was critical for the response because the anti-CD44 Ab IM7.8.1 blocked the effect on NF-kappa B. HA fragments activated the I kappa B kinase complex, and the effect on a kappa B-linked reporter gene was blocked in T-24 cells expressing dominant negative I kappa B kinases 1 or 2…”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD44 Monoclonal Antibody (Clone: IM7.8.1) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse/human CD44 Monoclonal Antibody (Clone: IM7.8.1), Rat IgG2b Kappa from: In Vivo Grade Recombinant Anti-mouse/human CD44 Monoclonal Antibody (Clone: IM7.8.1), Rat IgG2b Kappa: PA007622.r2b