Anti-Mouse MAdCAM-1 Antibody (Clone:MECA-367) | PA007581.r2a
$150.00 – $900.00
Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007581.r2a |
|---|---|
| Product Name | In Vivo Grade Recombinant Anti-mouse MAdCAM-1 Monoclonal Antibody (Clone: MECA-367), Rat IgG2a Kappa |
| Data Sheet | 📄 Download Product Data Sheet (PDF) |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | Mucosal addressin cell adhesion molecule-1 |
| Clone | MECA-367. |
| Isotype | Rat IgG2a Kappa. |
| Source/Host | The anti-mouse MAdCAM-1 monoclonal antibody (clone: MECA-367) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant rat monoclonal antibody (clone: MECA-367) specifically binds to mouse MAdCAM-1. |
| Applications | ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MAdCAM-1 protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The in vivo grade recombinant anti-mouse MAdCAM-1 monoclonal antibody of clone MECA-367 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007581.r2a: In Vivo Grade Recombinant Anti-mouse MAdCAM-1 Monoclonal Antibody (Clone: MECA-367), Rat IgG2a Kappa
Recombinant Rat IgG2a Monoclonal Antibody.
References of Anti-Mouse MAdCAM-1 Monoclonal Antibody (Clone: MECA-367)
1. Genomic Modifiers of Natural Killer Cells, Immune Responsiveness and Lymphoid Tissue Remodeling Together Increase Host Resistance to Viral Infection.
Gillespie, A. L., et al.PLoS Pathog. 2016 Feb 4;12(2):e1005419. doi: 10.1371/journal.ppat.1005419. PMID: 26845690
“Briefly, frozen sections (6 μm) were fixed with a 1:1 mix of acetone/ethanol(100%) and stained with Abs against B220 (RA3-632, BD Bioscience), CD3 (145-2C11, Ebiosciences), MAdCAM-1 (MECA-367, BioXcell), CD209b/SIGN-R1 (eBio22D1, Ebiosciences), NKp46 (polyclonal, R&D systems), and CD169 (MOMA-1, AbDSerotec). …As the extent of viral control differs in MA/My and Tg1 spleens (Fig 4), we considered that apparent differences in splenic SLO integrity could be due to the variance in viral titers. …Abs and isotype controls for flow cytometry were purchased from BD Biosciences, Biolegend, and R&D Systems. …Spleen and liver gDNA samples were prepared using a Gentraprep kit. …We gratefully acknowledge the contribution of DartMouse to genome-wide genotyping performed in this study.”
2. Effector lymphocyte-induced lymph node-like vasculature enables naive T-cell entry into tumours and enhanced anti-tumour immunity.
Peske, J. D., et al.Nat Commun. 2015 May 13;6:8114. doi: 10.1038/ncomms8114. PMID: 25968334
“In both tumour sites, PNAd+ vessels coexpressed MAdCAM-1 and VCAM-1 …Naive OT-I cells were transferred into WT mice with i.p. B16-OVA tumours pre-treated with anti-MAdCAM-1 antibody or Rat IgG isotype control (h), or anti-PNAd antibody or Rat IgM isotype control (i). …To determine whether infiltration was mediated by L-selectin binding to MAdCAM-1 or PNAd, we transferred OT-I cells into i.p. tumour-bearing mice pretreated to block luminally expressed molecules with injection of either anti-MAdCAM-1, anti-PNAd or isotype control. …MAdCAM-1 blockade reduced infiltration into Peyer’s patches but had no effect on tumour infiltration. …MAdCAM-1 did not contribute to naive T-cell infiltration, however, perhaps because it is not properly posttranslationally modified to serve as an L-selectin ligand.”
For more technical references or data sheets regarding the Recombinant Anti-Mouse MAdCAM-1 Monoclonal Antibody (Clone: MECA-367) formats, please contact our scientific support team at message@sydlabs.com.
In Vivo Grade Recombinant Anti-mouse MAdCAM-1 Monoclonal Antibody (Clone: MECA-367), Rat IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse MAdCAM-1 Monoclonal Antibody (Clone: MECA-367), Rat IgG2a Kappa: PA007581.r2a
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