Recombinant Anti-mouse IL-12 Monoclonal Antibody (R1-5D9), In Vivo PA007574.r2a Syd Labs

Anti-Mouse IL-12 Antibody (Clone:R1-5D9) | PA007574.r2a

$150.00 – $900.00

Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007574.r2a
Product Name	In Vivo Grade Recombinant Anti-mouse IL-12 Monoclonal Antibody (Clone: R1-5D9), Rat IgG2a Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12
Clone	R1-5D9.
Isotype	Rat IgG2a Kappa.
Source/Host	The anti-mouse IL-12 monoclonal antibody (clone: R1-5D9) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant rat monoclonal antibody (clone: R1-5D9) specifically binds to mouse IL-12.
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse IL-12 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-mouse IL-12 monoclonal antibody of clone R1-5D9 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007574.r2a: In Vivo Grade Recombinant Anti-mouse IL-12 Monoclonal Antibody (Clone: R1-5D9), Rat IgG2a Kappa

Recombinant Rat IgG2a Monoclonal Antibody.

References of Anti-Mouse IL-12 Monoclonal Antibody (Clone: R1-5D9)

1. Cutaneous Leishmaniasis Induces a Transmissible Dysbiotic Skin Microbiota that Promotes Skin Inflammation.
Gimblet, C., et al.Cell Host Microbe. 2017 Jul;22(1):13-24.e4. doi: 10.1016/j.chom.2017.06.006. PMID: 28669672
“To rule out the possibility that the increase in Streptococcus in non-healing BALB/c mice was due to differences in the mouse strain, we depleted IL-12 in C57BL/6 mice, which leads to non-healing lesions similar to those seen in BALB/c mice. …As expected, anti-IL-12 mAb-treated mice developed large non-healing lesions. …In specified experiments, mice were treated with 500µg of anti-IL-12 mAb (BioXcell, clone R1‒5D9, RRID:AB_1107700) one day prior to infection and then twice per week for the duration of the experiment. …Some of these severe forms of disease can be mimicked in mice, which can develop healing or non-healing disease following L. major infection depending upon whether a dominant Th1 or Th2 response develops. …Bacterial diversity was significantly lower in lesions compared to unaffected contralateral skin and adjacent skin sites, as measured by the observed species-level operational taxonomic units (OTUs) and Shannon Diversity indices.”

2. LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6.
Choi, Y. S., et al.Nat Immunol. 2015 Sep;16(9):980-90. doi: 10.1038/ni.3226. PMID: 26214741
“LEF-1 and TCF-1 coordinated such differentiation by two general mechanisms. …First, they established the responsiveness of naive CD4+ T cells to TFH cell signals. …Second, they promoted early TFH differentiation via the multipronged approach of sustaining expression of IL-6Rα and gp130, enhancing ICOS expression, and promoting expression of Bcl6. …Both in the steady-state and during early TFH differentiation, LEF-1/TCF-1 promote CD4+ T cells to develop into TFH cells by binding to Il6ra and Il6st to activate and/or to Prdm1 to repress. …Enriched TCF-1 binding was detected at the indicated gene loci in both WT and TCF-1-deficient TFH cells, and shown is ratio of enrichment in WT to Tcf7−/− TFH cells.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse IL-12 Monoclonal Antibody (Clone: R1-5D9) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse IL-12 Monoclonal Antibody (Clone: R1-5D9), Rat IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse IL-12 Monoclonal Antibody (Clone: R1-5D9), Rat IgG2a Kappa: PA007574.r2a