Anti-Mouse / Human / Rat CD91 (LRP1) Antibody (Clone:11H4) | PA007582.m1

Description

PA007582.m1: In Vivo Grade Recombinant Anti-mouse/human/rat CD91 (LRP1) Monoclonal Antibody (Clone: 11H4), Mouse IgG1 Kappa

Recombinant Mouse IgG1 Monoclonal Antibody.

References of Anti-Mouse CD91 (LRP1) Monoclonal Antibody (Clone: 11H4)

1. Urokinase-type Plasminogen Activator (uPA) Binding to the uPA Receptor (uPAR) Promotes Axonal Regeneration in the Central Nervous System.
Merino, P., et al.J Biol Chem. 2017 Feb 17;292(7):2741-2753. doi: 10.1074/jbc.M116.761650. PMID: 27986809
“To test this hypothesis, first we studied the expression of LRP1 in the model of axonal injury used in this study with a previously described mouse monoclonal antibody directed against the last 13 amino acids of the intracellular domain of LRP1 (11H4). …The RAP and antibodies against LRP1, directed to either the last 13 amino acids of its intracellular domain (monoclonal 11H4) or to its extracellular ligand-binding domains (polyclonal R2629), were kind gifts from Dr. Dudley K. Strickland (University of Maryland; Baltimore). …For siRNA preparations, DIV 3 WT cerebral cortical neurons were incubated with 3 μm LRP1 siRNA or with non-targeting siRNA following the manufacturer’s instructions, and 4 days later they were stained with a monoclonal antibody directed against LRP1’s intracellular domain (11H4). …WT cerebral cortical neurons were incubated 0–60 min with 5 nm uPA or 0–5 min with uPA alone or in combination with 20 mg/ml β1 integrin blocking antibodies. …Cerebral cortical neurons were cultured from E16 to E18 WT, uPAR−/−, and PlauGFDhu/GFDhu mice as described elsewhere.”

2. Tissue-type plasminogen activator suppresses activated stellate cells through low-density lipoprotein receptor-related protein 1.
Kang, L. I., et al.Lab Invest. 2015 Oct;95(10):1117-29. doi: 10.1038/labinvest.2015.94. PMID: 26237273
“FPRCK-TPA is tested to have zero enzymatic activity by functional assay (personal communication with the company). Antibodies used for immunohistolabeling and Western blotting include: α-SMA (Clone 1A4; Dako, Carpinteria, CA, USA; Sigma-Aldrich, St Louis, MO, USA), p-LRP1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), LRP1 (Clone 11H4; ATCC, Manassas, VA, USA; #3501, American Diagnostica), Collagen I (Abcam, Cambridge, MA), fibrinogen (Molecular Innovations), p-Akt (Thr308) and total Akt (both Cell Signaling Technology, Danvers, MA, USA), p-ERK1/2 and total ERK1/2 (Cell Signaling Technology). …One hundred micrograms of lysate from each treatment were incubated with the monoclonal antibody against LRP1 (11H4) or IgG control at 4 °C overnight with rotation. …Proteins were resolved on 10% SDS-polyacrylamide gel, and analyzed by western blotting as described above, using anti-phospho-tyrosine (1:250; BD Biosciences, San Jose, CA, USA) and anti–LRP1 (1:1000; 11H4) antibodies. …Isolated total RNA was DNase I-treated using Turbo DNase kit (Life Technologies, Grand Island, NY, USA) according to the manufacturer’s instructions. …PCR consisted of 30 cycles at 94 °C for 1 min, 59 °C for 1 min, and 72 °C for 1 min, followed by a final extension step at 72 °C for 7 min.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD91 (LRP1) Monoclonal Antibody (Clone: 11H4) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse/human/rat CD91 (LRP1) Monoclonal Antibody (Clone: 11H4), Mouse IgG1 Kappa from: In Vivo Grade Recombinant Anti-mouse/human/rat CD91 (LRP1) Monoclonal Antibody (Clone: 11H4), Mouse IgG1 Kappa: PA007582.m1