Anti-Mouse CD11a (LFA-1 alpha) Antibody (Clone:FD441.8) | PA007597.r2b
$150.00 – $900.00
Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007597.r2b |
|---|---|
| Product Name | In Vivo Grade Recombinant Anti-mouse CD11a (LFA-1 alpha) Monoclonal Antibody (Clone: FD441.8), Rat IgG2b Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | ITGAL, LFA-1, LFA1A, Leukocyte function-associated molecule 1 alpha chain |
| Clone | FD441.8. |
| Isotype | Rat IgG2b Kappa. |
| Source/Host | The anti-mouse CD11a (LFA-1 alpha) monoclonal antibody (clone: FD441.8) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant rat monoclonal antibody (clone: FD441.8) specifically binds to mouse CD11a (LFA-1 alpha). |
| Applications | ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD11a (LFA-1 alpha) protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The in vivo grade recombinant anti-mouse CD11a (LFA-1 alpha) monoclonal antibody of clone FD441.8 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007597.r2b: In Vivo Grade Recombinant Anti-mouse CD11a (LFA-1 alpha) Monoclonal Antibody (Clone: FD441.8), Rat IgG2b Kappa
Recombinant Rat IgG2b Monoclonal Antibody.
References of Anti-Mouse CD11a (LFA-1α) Monoclonal Antibody (Clone: FD441.8)
1. Pancreatic islet xenograft survival in mice is extended by a combination of alpha-1-antitrypsin and single-dose anti-CD4/CD8 therapy.
Ashkenazi, E., et al.PLoS One. 2013 May 22;8(5):e63625. doi: 10.1371/journal.pone.0063625. PMID: 23717456
“Subtherapeutic co-stimulation blockade included an equal mixture of anti-LFA-1 and anti-CD154 monoclonal antibodies (MR-1 and FD441.8, respectively, BioXCell, West Lebanon, NH, USA), each at 25µl/injection at the concentration of 1.25 mg/ml, one day before transplantation and every three days thereafter. …Explanted kidneys carrying implants were fixed in 10% formalin (Sigma) for 24 h and transferred into 70% ethanol. …Rat pancreatic islets (50/well in 48-well plates in triplicate) were cultured with medium alone or with recombinant IL-1β (10 ng/ml, R&D Systems), in the presence or absence of a 1 h pretreatment with hAAT (0.5 mg/ml). …In all experiments, the hAAT group is comprised of recipient mice that are transgenic for lung-derived hAAT. …In order to achieve a robust immune response and improve detection of changes in DLN, as well as to encourage responses with low variability, skin xenotransplantation was performed.”
2. Antibody-mediated B-cell depletion before adoptive immunotherapy with T cells expressing CD20-specific chimeric T-cell receptors facilitates eradication of leukemia in immunocompetent mice.
James, S. E., et al.Blood. 2009 Dec 24;114(27):5454-63. doi: 10.1182/blood-2009-08-232967. PMID: 19880489
“The blocking anti–LFA-1 (lymphocyte function–associated antigen–1) antibody FD441.8 was obtained from Bio X Cell. …In LFA-1–blocking experiments, T cells were labeled at 2 × 107 cells/mL in PBS 2.5mM EDTA containing 50 μg/mL FD441.8 at 4°C for 30 minutes, followed by washing twice in PBS. …For analysis of T-cell/B-cell conjugates, T cells were labeled with 1μM DDAO-SE (Invitrogen) and B cells or BM185-mCD20 was labeled with 0.1μM CFSE (Invitrogen). …The percentage of T cells in conjugates was calculated by dividing the percentage of CFSE+DDAO+ conjugates by the total percentage of DDAO+ events. …Luciferase activity was analyzed in mice anesthetized with isoflurane 10 minutes after intraperitoneal injection of d-luciferin potassium salt (Caliper Life Sciences) at 150 mg/kg.”
For more technical references or data sheets regarding the Recombinant Anti-Mouse CD11a (LFA-1α) Monoclonal Antibody (Clone: FD441.8) formats, please contact our scientific support team at message@sydlabs.com.
In Vivo Grade Recombinant Anti-mouse CD11a (LFA-1 alpha) Monoclonal Antibody (Clone: FD441.8), Rat IgG2b Kappa from: In Vivo Grade Recombinant Anti-mouse CD11a (LFA-1 alpha) Monoclonal Antibody (Clone: FD441.8), Rat IgG2b Kappa: PA007597.r2b
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