Catalog No.	PA007711.m1
Product Name	Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2) | PA007711.m1
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	T cell receptor
Clone	1B2.
Isotype	Mouse IgG1 kappa.
Source/Host	The anti-mouse 2C TCR monoclonal antibody (clone: 1B2) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: 1B2) specifically binds to mouse 2C TCR .
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse 2C TCR protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-mouse 2C TCR monoclonal antibody of clone 1B2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007711.m1: In Vivo Grade Recombinant Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2), Mouse IgG1 Kappa

Recombinant mouse IgG1 monoclonal antibody.

References of Anti-mouse 2C TCR Monoclonal Antibody:

STING-dependent cytosolic DNA sensing mediates innate immune recognition of immunogenic tumors.
Woo, S. R., et al. Immunity. 2014 Nov 20;41(5):830-42. doi: 10.1016/j.immuni.2014.10.017. PMID: 25517615
Because MyD88 can function in a T cell-intrinsic fashion (Zhou et al., 2009), we performed adoptive transfer of wild-type (WT) CFSE-labeled 2C TCR Tg T cells (that are specific for the model antigen SIY) into WT or Myd88?/? mice and challenged with B16.SIY tumors. …To evaluate in more detail the mechanism by which absence of host STING resulted in impaired antitumor T cell responses in vivo, we adoptively transferred CFSE-labeled 2C TCR Tg T cells into WT and STING-deficient mice and measured T cell proliferation by CFSE dilution after B16.SIY challenge. …2C TCR Tg CD8+ T cells were isolated from spleens and lymph nodes of 2C/RAG2?/? mice by using magnetic beads. …After 1 day, recipient mice received 106 B16.SIY cells, and 5 days later splenocytes from recipient mice were analyzed after staining with anti-mouse CD8?-APC and biotin-labeled anti-2C-TCR (1B2) with SA-PE by flow cytometry to assess CFSE dilution. …Splenocytes were plated at 106 cells per well and stimulated overnight with SIY peptide.
Tags: activity of Anti-Mouse 2C TCR Monoclonal; Anti-Mouse 2C TCR Monoclonal

Murine anti-third-party central-memory CD8(+) T cells promote hematopoietic chimerism under mild conditioning: lymph-node sequestration and deletion of anti-donor T cells.
Ophir, E., et al. Blood. 2013 Feb 14;121(7):1220-8. doi: 10.1182/blood-2012-07-441493. PMID: 23223359
To that end, stained 2c CD8+ HTCs and CB6 Tcms (“specific” Tcms, recognized specifically by the 2c TCR transgene) were adoptively transferred at the indicated levels into lethally (10Gy TBI) and sublethally (6.5Gy TBI) irradiated C57BL/6 mice. …Interestingly, a similar range was suggested by Obar et al, who quantitated naive antigen-specific CD8+ T cells and the early response to infection, and found that among 6 different specificities measured, the number of naive antigen-specific precursors ranged from ? 80 to 1200 cells/mouse.39 Thus, the number of 2c TCR Tg cells used is probably within the physiologic range. …In addition, the 2c-derived CD8+ cells can be identified using the clonotypic 1B2 antibody directed against their transgenic TCR. …Cells were stained with labeled antibodies specific for: CD3-PE/FITC/APC, CD8?-PE/FITC/APC, CD11b-PE/FITC/APC, CD19-PE/FITC/APC, CD62L-PE/FITC/APC, CD44-PE/FITC/APC, H-2Kb-PE/FITC, H-2Dd-PE/FITC, H-2Kk-PE/FITC (BD Pharmigen); 1B2 biotinylated, and streptavidin-APC (Jackson Laboratories). …Hematopoietic stem cell transplantation (HSCT) can offer a cure for patients with a variety of nonmalignant hematologic disorders, such as sickle cell anemia and thalassemia.
Tags: Anti-Mouse 2C TCR Monoclonal antibody for animal model; Anti-Mouse 2C TCR Monoclonal antibody for cancer research

KRAS G12V neoantigen specific T cell receptor for adoptive T cell therapy against tumors.
Lu, D., Chen, Y., Jiang, M., et al. Nat Commun. 2023 Oct 12;14(1):6389. doi: 10.1038/s41467-023-42010-1. PMID: 37828002
1?2C TCR-T cells show tumor suppression efficacy in a tumor-bearing mouse model and improved inhibition of tumor growth in combination with anti-PD-1 antibodies. …Of note, 1-2C TCR (TRAV7-2*02, TRBV13-2*01) is a public TCR that presents in each of the four mice and dominated KRAS-G12V-9 specific TCRs in Mus-T5 and Mus-TF1. …Responses of 1-2C TCR-T cells with wildtype CFPAC-1 cells or CFPAC-1 cells stably expressing HLA-A*11:01 (h), or with wildtype SW-620 cells or SW-620 cells stably expressing HLA-A*11:01 (i). …Many of the substitutions at V1 and V2 were tolerated for 1-2C TCR-T cells, as was also observed for the substitutions at V2 and G8 for 3-2E TCR-T cells. …The above analyses revealed that the 1-2C TCR has higher binding affinity and immune responsiveness against tumor cells than the 3-2E TCR, and 1-2C was thus selected for further in vivo anti-tumor evaluations.
Tags: Anti-Mouse 2C TCR Monoclonal antibody for mouse tumor model; Anti-Mouse 2C TCR Monoclonal for animal model

Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Nishimura, H., et al. Immunity. 1999 Aug;11(2):141-51. doi: 10.1016/s1074-7613(00)80089-8. PMID: 10485649
Anti-2C TCR?? clonotypic mAb, 1B2 (Kranz et al. 1984), and anti-mouse PD-1 mAb (J43) were biotinylated. …For immunochemistry, cryosections were pretreated with 2% BSA in PBS/0.05% Tween (PBS/T) for 30 min and incubated with biotinylated CD8a or 1B2 overnight. …This work was supported by grants from the Ministry of Education, Science, Sports, and Culture of Japan, from Searle Scientific Research Fellowship, and from Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists. …Unlike most of the lupus-prone mice, common autoantibodies represented by anti-double strand DNA (dsDNA) and rheumatoid factor (RF) were hardly detected in B6-PD-1?/? mice (unpublished data). …Most recently, the mice deficient for CD22, a typical ITIM-based negative regulator on B cells, were reported to develop high-titer anti-dsDNA antibodies of IgG2a as they aged with no evidence of pathogenesis.
Tags: Anti-Mouse 2C TCR Monoclonal for flow cytometry; Anti-Mouse 2C TCR Monoclonal in vivo

Structure-Based, Rational Design of T Cell Receptors.
Zoete, V., et al. Front Immunol. 2013 Sep 12;4:268. doi: 10.3389/fimmu.2013.00268. PMID: 24062738
Using the well-characterized 2C TCR/SIYR/H-2K(b) structure as a model system, we demonstrated that a binding free energy decomposition based on the MM-GBSA approach provides a detailed and reliable description of the TCR/pMHC interactions at the structural and thermodynamic levels. …To assess the ability of the MM-GBSA approach to identify quantitatively the hot-spots residues for the TCR/pMHC association, we performed a study of the 2C TCR/SIYR/H-2Kb system using both the CAS and BFED methods. …Our results also showed that BFED provided a more detailed and reliable description of the interactions between the TCR and pMHC molecules when including entropic terms. …A limitation, however, is that TCRs which bind tumor associated/self antigen are often of relatively low affinity. …It could thus be expected that mutations of CDR3s would be more likely to maintain peptide specificity than mutants of CDR1s and CDR2s.
Tags: function of Anti-Mouse 2C TCR Monoclonal; function of Anti-Mouse 2C TCR Monoclonal antibody

For more references about Anti-mouse 2C TCR Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2), Mouse IgG1 Kappa from: In Vivo Grade Recombinant Anti-mouse 2C TCR Monoclonal Antibody (Clone: 1B2), Mouse IgG1 Kappa: PA007711.m1 Syd Labs

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