MOPC-21 Mouse IgG2a Isotype Control Antibody, In vivo Grade Recombinant PA007155.M2A Ushelf

MOPC-21 Mouse IgG2a Isotype Control Antibody | PA007155.m2a

Name: Mouse IgG2a Isotype Control Antibody (Clone: MOPC-21) | Ushelf
Brand: Syd Labs
SKU: PA007155.m2a
Price: 195 USD
Availability: InStock
Rating: 5 (1 reviews)

$595.00

Recombinant isotype control antibodies and Fc silenced mutants from clone MOPC-21 good for in vitro and in vivo studies. The variable regions of the MOPC-21 antibody share the sequences with the antibodies against all members of the dengue virus complex, and the Epstein-Barr virus gp350 protein.

Details & Specifications
References

Catalog No.	PA007155.m2a
Product Name	In vivo Grade Recombinant MOPC-21 Mouse IgG2a Isotype Control Antibody
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Recombinant MOPC-21 Isotype Control, MOPC-21 Negative Control Antibody
Summary	The In vivo Grade Recombinant MOPC-21 Mouse IgG2a Isotype Control Antibody was produced in mammalian cells.
Clone	MOPC-21
Isotype	Mouse IgG2a, kappa
Applications	an isotype-matched negative control for mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Immunogen	N/A.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In vivo Grade Recombinant MOPC-21 Mouse IgG2a Isotype Control Antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Note	Recombinant isotype control antibodies and Fc silenced mutants from clone MOPC-21 good for in vitro and in vivo studies. The variable regions of the MOPC-21 antibody share the sequences with the antibodies against all members of the dengue virus complex, and the Epstein-Barr virus gp350 protein.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007155.m2a：Recombinant MOPC-21 Mouse IgG2a Isotype Control Antibody (Clone: MOPC-21), In vivo Grade

The in vivo grade recombinant MOPC-21 mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.

Background

The MOPC-21 antibody (IgG1, kappa) has been used for a long time as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The variable regions of the MOPC-21 antibody share the sequences with the antibodies against all members of the dengue virus complex, and the Epstein-Barr virus gp350 protein. The recombinant MOPC-21 IgG1 kappa isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. Mouse IgG1 isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions.

As an ideal recombinant mouse IgG1 isotype control, the recombinant MOPC-21 mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and completely mouse variable regions and the mouse IgG1 kappa constant regions. The mouse IgG1 kappa isotype control and mutants can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the engineered MOPC-21 mouse IgG1 isotype control mutants, mIgG1 isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated mIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the MOPC-21 mouse IgG1 isotype control and mutants are available.

References of MOPC-21 Isotype Control Antibodies

1. Programmed cell death ligand 2 regulates TH9 differentiation and induction of chronic airway hyperreactivity.
Kerzerho J, et al.J Allergy Clin Immunol. 2013 Apr;131(4):1048-57, 1057.e1-2. PMID: 23174661
“Differences between groups were analyzed by using the 2-tailed, unpaired Student t test and considered significant when the P value was less than .05. …Asthma is defined as a chronic inflammatory disease of the airways; however, the underlying physiologic and immunologic processes are not fully understood. …The aim of this study was to determine whether TH9 cells develop in vivo in a model of chronic airway hyperreactivity (AHR) and what factors control this development. …We have developed a novel chronic allergen exposure model using the clinically relevant antigen Aspergillus fumigatus to determine the time kinetics of TH9 development in vivo. …TH9 cells were detectable in the lungs after chronic allergen exposure.”

2. IL-17A and IL-2-expanded regulatory T cells cooperate to inhibit Th1-mediated rejection of MHC II disparate skin grafts.
Vokaer B, et al.PLoS One. 2013 Oct 11;8(10):e76040. PMID: 24146810
“The neutralizing anti-IL-17A monoclonal antibody and the Isotype control antibody were intraperitoneally injected at a dose of 400 μg twice a week after transplantation. …Total RNA was extracted from skin grafts using the MagnaPure LC RNA Isolation Kit III for tissue (Roche Diagnostics). …Number of mRNA copies was evaluated by using standard curve for each gene of interest and was normalized to β-actin as housekeeping gene. …Cell suspensions were incubated for 10 minutes with Fc block, stained for surface markers for 20 minutes, washed with Phosphate Buffer Saline/Bovine Serum Albumin 0.1%/NaN30.01%. …For in vitro suppression assay, WT CD4+ T cells were isolated using CD4 negative isolation kit from Miltenyi, and used as responder cells.”

3. Regulation of antinucleoprotein IgG by systemic vaccination and its effect on influenza virus clearance.
Lamere MW, et al.J Virol. 2011 May;85(10):5027-35. PMID: 21367900
“Irrelevant isotype-matched control antibodies were purchased from BioXcell. …Influenza virus-infected mice were sacrificed, and lungs were homogenized in PBS with penicillin-streptomycin. …Statistical analysis was performed using Prism 5.0a software. …To determine whether the 2009 pandemic H1N1 virus is susceptible to antibody-mediated NP immunity, we first vaccinated C57BL/6 and antibody-deficient, AID-/- mice with recombinant purified influenza virus NP, based on the PR8 amino acid sequence, as described previously. …To determine how much NP was contained in the 2008/09 Fluzone preparation used prior to the 2009 H1N1 influenza virus pandemic, we performed a capture ELISA using a combination of monoclonal and polyclonal anti-NP antibodies and recombinant purified NP as a standard.”

For more technical references or data sheets regarding the MOPC-21 Isotype Control Antibodies formats, please contact our scientific support team at message@sydlabs.com.