[KO Validated] p53 Rabbit mAb (A22449)

Catalog No.	A22449
Product Name	[KO Validated] p53 Rabbit mAb (A22449)
Supplier Name	ABclonal, Inc.
Brand Name	Abclonal
Synonyms	P53; BCC7; LFS1; BMFS5; TRP53
Gene Name	TP53
Protein Name	TP53
Uniprot/Swissprot ID	P04637
Entrez GeneID	7157
Clone	ARC50968
Clonality	Monoclonal
Source/Host	Rabbit
Applications	WB, IHC-P
Reactivity	Human, Rat
Conjugate	Unconjugated
Note	Products will be shipped from the warehouse in Massachusetts. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com.
Order Offline	Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com

Description

A22449: [KO Validated] p53 Rabbit mAb

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Immunogen Information about [KO Validated] p53 Rabbit mAb (A22449)

Immunogen：Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (NP_000537.3).
Sequence：MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
Gene ID：7157
Swiss prot：P04637
Synonyms：P53; BCC7; LFS1; BMFS5; TRP53; 53
Calculated MW：44kDa
Observed MW：53kDa

Images of [KO Validated] p53 Rabbit mAb (A22449)

Western blot analysis of extracts from wild type(WT) and p53 knockout (KO) 293T(KO) cells, using p53 antibody (A22449) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Immunohistochemistry analysis of paraffin-embedded human oophoroma using [KO Validated] p53 Rabbit mAb (A22449) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Please remember our product information: [KO Validated] p53 Rabbit mAb (Catalog Number: A22449) Abclonal

Additional information

Ship from Country	USA
Size	20 ul, 100 ul, 200 ul, 50 ul

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