Cytokeratin 5 (CK5) Rabbit mAb (A11396)
$148.00 – $548.00
Abclonal Cytokeratin 5 (CK5) Rabbit mAb (Catalog Number: A11396) encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues.
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| Catalog No. | A11396 |
|---|---|
| Product Name | Cytokeratin 5 (CK5) Rabbit mAb (A11396) |
| Supplier Name | ABclonal, Inc. |
| Brand Name | Abclonal |
| Synonyms | K5; CK5; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; KRT5A |
| Gene Name | KRT5 |
| Protein Name | KRT5 |
| Uniprot/Swissprot ID | P13647 |
| Gene ID | 3852 |
| Clone | ARC0585 |
| Clonality | Monoclonal |
| Source/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Note | Products will be shipped from the warehouse in Massachusetts. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com. |
| Order Offline | Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com |
Description
A11396: Cytokeratin 5 (CK5) Rabbit mAb
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Immunogen Information about Cytokeratin 5 (CK5) Rabbit mAb (A11396)
Immunogen：A synthetic peptide corresponding to a sequence within amino acids 491-590 of human Cytokeratin 5 (KRT5) (P13647).
Sequence：ISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS
Gene ID：3852
Swiss prot：P13647
Synonyms：K5; KRT5; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; KRT5A; Cytokeratin 5 (CK5)
Calculated MW：62kDa
Observed MW：62kDa
Images of Cytokeratin 5 (CK5) Rabbit mAb (A11396)
Western blot analysis of extracts of A-431 cells, using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Cytokeratin 5 (KRT5) Rabbit mAb (A11396) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Confocal imaging of paraffin-embedded Human skin using Cytokeratin 5 (CK5) Rabbit mAb (A11396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Mouse skin using Cytokeratin 5 (CK5) Rabbit mAb (A11396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Rat skin using Cytokeratin 5 (CK5) Rabbit mAb (A11396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
Please remember our product information: Cytokeratin 5 (CK5) Rabbit mAb (Catalog Number: A11396) Abclonal
