Biotinylated Anti-mouse CD28 Antibody (PV-1, Human IgG1 Fc Silent Kappa)

Catalog No.	PA007720.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: PV-1), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	PV-1
Isotype	Human IgG1 Fc Silent Kappa.
Source/Host	The site-directed biotinylated anti-mouse CD28 monoclonal antibody (clone: PV-1) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant PV-1 antibody specifically binds to mouse CD28.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The PV-1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007720.h1Fs-b: Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: PV-1), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

References of Anti-Mouse CD28 Monoclonal Antibody (Clone: PV-1):
1. CRK proteins selectively regulate T cell migration into inflamed tissues

Huang, Y., et al. J Clin Invest. 2015 Mar 2;125(3):1019-32. PMID: 25621495

“Effector T cell migration into inflamed sites greatly exacerbates tissue destruction and disease severity in inflammatory diseases, and signaling pathways coordinate these functions downstream of chemokine receptors.”

2. Dual-specificity phosphatase 6 regulates CD4+ T-cell functions and restrains spontaneous colitis in IL-10-deficient mice

Such, J., et al. Mucosal Immunol. 2015 May;8(3):505-15. PMID: 25227984

“Mitogen-activated protein kinase (MAPK) phosphatases are dual-specificity phosphatases (DUSPs) that dephosphorylate phosphothreonine and phosphotyrosine residues within MAPKs, regulating CD4+ T-cell functions.”

3. CXCL13 antibody for the treatment of autoimmune disorders

Klimatcheva, E., et al. BMC Immunol. 2015 Feb 12;16:6. PMID: 25879435

“Homeostatic B Cell-Attracting chemokine 1 (BCA-1) / CXCL13 is constitutively expressed in secondary lymphoid organs and serves as a ligand for the CXCR5 receptor expressed on mature B cells and Tfh cells.”

4. Novel aminotetrazole derivatives as selective STAT3 non-peptide inhibitors

Pallandre, J. R., et al. Eur J Med Chem. 2015 Oct 20;103:163-74. PMID: 26352675

“The development of inhibitors blocking STAT3 transcriptional activity is a promising therapeutic approach against cancer and inflammatory diseases, with selectivity against STAT1 being crucial.”

5. The ion channel TRPV1 regulates the activation and proinflammatory properties of CD4+ T cells

Bertin, S., et al. Nat Immunol. 2014 Nov;15(11):1055-1063. PMID: 25282159

“TRPV1 is a Ca(2+)-permeable channel studied mostly as a pain receptor in sensory neurons, and its role extends to regulating the activation and proinflammatory properties of CD4+ T cells.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD28 Monoclonal Antibody (Clone: PV-1) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51)
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)

Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)

Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51) for flow cytometry
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1) for flow cytometry

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry