Biotinylated Anti-mouse CD28 Antibody (37.51, Human IgG1 Fc Silent Kappa)

Catalog No.	PA007719.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	37.51
Isotype	Human IgG1 Fc Silent Kappa.
Source/Host	The site-directed biotinylated anti-mouse CD28 monoclonal antibody (clone: 37.51) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant 37.51 antibody specifically binds to mouse CD28.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The 37.51 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007719.h1Fs-b: Biotinylated Recombinant Anti-mouse CD28 Monoclonal Antibody (Clone: 37.51), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

References of Anti-Mouse CD28 Monoclonal Antibody (Clone: 37.51):
1. Targeting abatacept-resistant T-helper-17 cells by aldehyde dehydrogenase inhibition

Tokifuji, Y., et al. iScience. 2023 Dec 9;27(1):108646. PMID: 38226171

“IL-17-producing helper T (Th17) cells are long-lived and serve as central effector cells in chronic autoimmune diseases, and targeting pathways involved in their persistence provides potential therapeutic strategies.”

2. A non-catalytic role of IPMK is required for PLCγ1 activation in T cell receptor signaling by stabilizing the PLCγ1-Sam68 complex

Hong, S., et al. Cell Commun Signal. 2024 Oct 30;22(1):526. PMID: 39478550

“Phospholipase C gamma 1 (PLCγ1) is an important mediator of the T cell receptor (TCR) and growth factor signaling, playing a critical role in downstream immune activation pathways.”

3. Water channel aquaporin 4 is required for T cell receptor mediated lymphocyte activation

Nicosia, M., et al. J Leukoc Biol. 2023 Jun 1;113(6):qiad010. PMID: 36805947

“Aquaporins are ubiquitously expressed transmembrane water channels implicated in physiological functions, and aquaporin 4 (AQP4) expression on T cells relates to T cell-mediated immune responses.”

4. SEL1L preserves CD8+ T-cell survival and homeostasis by fine-tuning PERK signaling and the IL-15 receptor-mediated mTORC1 axis

Gao, Y., et al. Cell Mol Immunol. 2023 Oct;20(10):1232-1250. PMID: 37644166

“SEL1L-mediated endoplasmic reticulum-associated degradation (ERAD) plays critical roles in controlling protein homeostasis and regulating peripheral T-cell survival and homeostasis.”

5. Sirtuin 5 is Dispensable for CD8+ T Cell Effector and Memory Differentiation

Duan, Q., et al. Front Cell Dev Biol. 2021 Dec 13;9:761193. PMID: 34966740

“CD8+ T cell effector and memory differentiation is tightly controlled at multiple levels including transcriptional, metabolic, and epigenetic regulation during immune responses.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD28 Monoclonal Antibody (Clone: 37.51) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51)
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1)

Syd Labs provides the following in vivo grade recombinant anti-human CD28 monoclonal antibodies:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8)
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3)
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3)

Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: 37.51) for flow cytometry
Recombinant Anti-mouse CD28 monoclonal antibody (Clone: PV-1) for flow cytometry

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.2) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 15E8) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: 9.3) for flow cytometry
Recombinant Anti-human CD28 monoclonal antibody (Clone: CD28.3) for flow cytometry