Anti-Mouse / Human TYRP1 / TRP1 (gp75) Antibody (Clone:TA99) | PA007556.m2a
$150.00 – $900.00
Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007556.m2a |
|---|---|
| Product Name | In Vivo Grade Recombinant Anti-mouse/human TYRP1/TRP1 (gp75) Monoclonal Antibody (Clone: TA99), Mouse IgG2a Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | DHICA oxidase, Catalase B, Glycoprotein 75, Melanoma antigen gp75, Tyrosinase-related protein 1, TRP, TRP-1, TRP1 |
| Clone | TA99. |
| Isotype | Mouse IgG2a kappa. |
| Source/Host | The anti-mouse TYRP1/TRP1 (gp75) monoclonal antibody (clone: TA99) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant mouse monoclonal antibody (clone: TA99) specifically binds to mouse TYRP1/TRP1 (gp75). |
| Applications | ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse TYRP1/TRP1 (gp75) protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The in vivo grade recombinant anti-mouse TYRP1/TRP1 (gp75) monoclonal antibody of clone TA99 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007556.m2a: In Vivo Grade Recombinant Anti-mouse/human TYRP1/TRP1 (gp75) Monoclonal Antibody (Clone: TA99), Mouse IgG2a Kappa
Recombinant mouse IgG2a Monoclonal Antibody.
References of Anti-Mouse TYRP1/TRP1 (gp75) Monoclonal Antibody (Clone: TA99)
1. BLOC-2 targets recycling endosomal tubules to melanosomes for cargo delivery.
Dennis, M. K., et al.J Cell Biol. 2015 May 25;209(4):563-77. doi: 10.1083/jcb.201410026. PMID: 26008744
“In BLOC-2-deficient melanocytes, the melanosomal protein TYRP1 was largely depleted from pigment granules and underwent accelerated recycling from endosomes to the plasma membrane and to the Golgi. …Other melanosome cargoes, such as TYR-related protein-1 (TYRP1) and oculocutaneous albinism type 2 (OCA2), and a smaller cohort of TYR, exploit a distinct pathway that requires BLOC-1 for cargo exit from vacuolar early endosomes. …Besides the aberrant localization to early endosomes and the Golgi, TYRP1 levels at the plasma membrane—as assessed by flow cytometry—were increased (by 3.5–5-fold) in BLOC-2-/- and BLOC-2-/-C relative to BLOC-2+/+ cells. …To directly assess TYRP1 recycling to the Golgi, we used FRAP after expression of TYRP1-EGFP. …Labeling was particularly evident on tubules that emanated from vacuolar endosomes and that closely apposed and/or contacted melanosomes, at least some of which were associated with AP-1 and/or contained TYRP1.”
2. Key regulatory role of dermal fibroblasts in pigmentation as demonstrated using a reconstructed skin model: impact of photo-aging.
Duval, C., et al.PLoS One. 2014 Dec 9;9(12):e114182. doi: 10.1371/journal.pone.0114182. PMID: 25490395
“For eumelanins which give rise to brown or black pigment (comprising DHICA-melanin and DHI-melanin), TRP-1 (tyrosinase related protein-1) and TRP-2 (tyrosine related protein-2, or DCT for DOPA-chrome tautomerase) are involved. …To detect proteins involved in melanogenesis, primary monoclonal antibodies (MoAb), Ta99 specific to human TRP-1 (Signet, Dedham, MA, USA) and T311 specific to human Tyrosinase (Novocastra, Newcastle, UK) were used. …For fluorescent immunochemistry, Ta99 (dilution 1/50) which recognizes TRP-1 was applied to cryosections for 30 min at room temperature (RT). …Primary monoclonal antibody (MoAb) TA99 specific to TRP-1 (Signet) was applied (dilution 1/100) for 60 min at RT. …TRP-1 staining (red) of cross-sections shows melanocytes positioned at the basal layer of the epidermis in both 3D systems.”
For more technical references or data sheets regarding the Recombinant Anti-Mouse TYRP1/TRP1 (gp75) Monoclonal Antibody (Clone: TA99) formats, please contact our scientific support team at message@sydlabs.com.
In Vivo Grade Recombinant Anti-mouse/human TYRP1/TRP1 (gp75) Monoclonal Antibody (Clone: TA99), Mouse IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse/human TYRP1/TRP1 (gp75) Monoclonal Antibody (Clone: TA99), Mouse IgG2a Kappa: PA007556.m2a
