Anti-Mouse / Human Mac-2 (Galectin-3) Antibody (Clone:M3/38.1.2.8HL.2) | PA007563.r2a

Catalog No.	PA007563.r2a
Product Name	In Vivo Grade Recombinant Anti-mouse/human Mac-2 (Galectin-3) Monoclonal Antibody (Clone: M3/38.1.2.8HL.2), Rat IgG2a Kappa
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, ?BP
Clone	M3/38.1.2.8HL.2.
Isotype	Rat IgG2a kappa.
Source/Host	The anti-mouse/human Mac-2 (Galectin-3) monoclonal antibody (clone: M3/38.1.2.8HL.2) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant rat monoclonal antibody (clone: M3/38.1.2.8HL.2) specifically binds to mouse/human Mac-2 (Galectin-3).
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse/human Mac-2 (Galectin-3) protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-mouse/human Mac-2 (Galectin-3) monoclonal antibody of clone M3/38.1.2.8HL.2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007563.r2a: In Vivo Grade Recombinant Anti-mouse/human Mac-2 (Galectin-3) Monoclonal Antibody (Clone: M3/38.1.2.8HL.2), Rat IgG2a Kappa

Recombinant rat IgG2a Monoclonal Antibody.

References of Anti-Mouse Mac-2 (Galectin-3) Monoclonal Antibody (Clone: M3/38.1.2.8HL.2)

1. Effective rational humanization of a PASylated anti-galectin-3 Fab for the sensitive PET imaging of thyroid cancer in vivo.
Scheckenbach, K., et al.Eur J Nucl Med Mol Imaging. 2021 Oct;48(12):3854-3866. doi: 10.1007/s00259-021-05357-3. PMID: 33795750
“The rat monoclonal antibody M3/38 (M3/38.1.2.8HL.2 clone) was used to generate a chimeric Fab for in vivo targeting of human galectin-3 in thyroid cancer xenografts. PASylation of the anti-galectin-3 Fab extended serum half-life and improved tumor uptake in vivo. In vivo PET imaging with 89Zr-labeled PASylated Fab showed specific accumulation in galectin-3-positive tumors. Biodistribution studies in mice confirmed low off-target binding post-Fab administration. Tumor-to-muscle ratios were significantly higher in galectin-3-expressing models.”

2. A galectin-3 ligand corrects the impaired function of human CD4 and CD8 tumor-infiltrating lymphocytes and favors tumor rejection in mice.
Banh, R. S., et al.Cancer Res. 2011 Jan 1;71(1):74-81. doi: 10.1158/0008-5472.CAN-10-1644. PMID: 20719885
“CD8+ TIL treated with an anti-galectin-3 antibody (M3/38 clone) had increased IFN-γ secretion in vivo. Tumor-bearing mice vaccinated with tumor antigen received GCS-100 injections to disrupt galectin-3 in vivo. In vivo GCS-100 treatment led to tumor rejection in 50% of vaccinated mice. Galectin-3 blockade enhanced T cell mobility and effector function in the tumor microenvironment. Non-vaccinated mice showed no response to GCS-100 alone in vivo.”

3. Chronic upregulation of activated microglia immunoreactive for galectin-3/Mac-2 and nerve growth factor following diffuse axonal injury.
Byrnes, K. R., et al.J Neurotrauma. 2010 Jul;27(7):1329-40. doi: 10.1089/neu.2009.1235. PMID: 20507613
“Activated microglia immunoreactive for galectin-3/Mac-2 were quantified in vivo after diffuse axonal injury. Galectin-3/Mac-2+ microglia were most abundant 1 day post-injury in mouse brain tissue. In vivo levels of galectin-3/Mac-2+ cells remained elevated at 30 days after injury. Colocalization with NGF was observed in galectin-3/Mac-2+ microglia in vivo. Sham-injured mice showed minimal galectin-3/Mac-2 immunoreactivity.”

4. Compartmentalized reactivity of M3/38 (anti-Mac-2) and M3/84 (anti-Mac-3) in the uterus of pregnant mice.
Peel, S., et al.J Reprod Fertil. 1993 Jul;98(3):511-6. doi: 10.1530/jrf.0.0980511. PMID: 8501724
“Cryostat sections of uteri from pregnant mice were probed with M3/38 (anti-Mac-2) antibody in vivo. M3/38 reacted with decidual cells surrounding the embryo on day 8 of pregnancy. By day 15, placenta and decidua basalis were intensely positive for M3/38 in vivo. Embryo tissue remained negative for M3/38 immunoreactivity on day 15. Uterine leucocytes showed strong M3/38 staining throughout pregnancy.”

5. Mac-2, a novel 32,000 Mr mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies.
Ho, M. K., et al.J Immunol. 1982 Oct;129(4):1674-9. PMID: 6173426
“Thioglycollate-elicited macrophages were labeled in vivo with 125I-M3/38 antibody. M3/38 antibody recognized Mac-2 antigen on macrophage surfaces in vivo. Saturation binding showed 1.7 × 10^5 Mac-2 sites per macrophage in vivo. Co-precipitation confirmed M3/38 and M3/31 bind the same Mac-2 molecule in vivo. Mac-2 was synthesized by macrophages as detected by [35S]-methionine labeling in vivo.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse Mac-2 (Galectin-3) Monoclonal Antibody (Clone: M3/38.1.2.8HL.2) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse/human Mac-2 (Galectin-3) Monoclonal Antibody (Clone: M3/38.1.2.8HL.2), Rat IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse/human Mac-2 (Galectin-3) Monoclonal Antibody (Clone: M3/38.1.2.8HL.2), Rat IgG2a Kappa: PA007563.r2a