Recombinant Anti-mouse Dendritic Cell Marker Monoclonal Antibody (33D1), In Vivo PA007602.r2b Syd Labs
In Vivo Grade Recombinant Anti-mouse Dendritic Cell Marker Monoclonal Antibody (Clone 33D1), Rat IgG2b Kappa

Anti-Mouse Dendritic Cell Marker Antibody (Clone:33D1) | PA007602.r2b

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Recombinant Anti-mouse Dendritic Cell Marker Monoclonal Antibody (33D1), In Vivo PA007602.r2b Syd Labs

Anti-Mouse Dendritic Cell Marker Antibody (Clone:33D1) | PA007602.r2b

$150.00 – $900.00

In stock

$150.00 – $900.00

Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

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Catalog No. PA007602.r2b
Product Name In Vivo Grade Recombinant Anti-mouse Dendritic Cell Marker Monoclonal Antibody (Clone: 33D1), Rat IgG2b Kappa
Supplier Name Syd Labs, Inc.
Brand Name Syd Labs
Synonyms DC Marker, Dendritic Cell Marker, 33D1, DCIR2 (dendritic cell inhibitory receptor 2)
Clone 33D1.
Isotype Rat IgG2b Kappa.
Source/Host The anti-mouse Dendritic Cell Marker monoclonal antibody (clone: 33D1) was produced in mammalian cells.
Specificity/Sensitivity The in vivo grade recombinant rat monoclonal antibody (clone: 33D1) specifically binds to mouse Dendritic Cell Marker.
Applications ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse Dendritic Cell Marker protein.
Form Of Antibody 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin < 1 EU per 1 mg of the protein by the LAL method.
Purity >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping The in vivo grade recombinant anti-mouse Dendritic Cell Marker monoclonal antibody of clone 33D1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007602.r2b: In Vivo Grade Recombinant Anti-mouse Dendritic Cell Marker Monoclonal Antibody (Clone: 33D1), Rat IgG2b Kappa

Recombinant Rat IgG2b Monoclonal Antibody.

References of Anti-Mouse Dendritic Cell Marker 33D1 Monoclonal Antibody (Clone: 33D1)

1. Effects of Dendritic Cell Subset Manipulation on Airway Allergy in a Mouse Model.
Murakami, R., et al.Int Arch Allergy Immunol. 2015;168(4):219-32. doi: 10.1159/000443237. PMID: 26855055
“Dendritic cells (DCs) serve an important role by providing an appropriate internal immune balance between Th1 and Th2 responses. …Indeed, we have previously shown that it is possible to deplete 33D1+ DCs and establish Th1 dominance by DEC-205+ DCs through the administration of a 33D1-specific monoclonal antibody (mAb) in vivo. …In contrast, Th2 dominance organized by the 33D1+ DC subset may be required to maintain a successful pregnancy. …In the present study, using the established allergic rhinitis (AR) mouse model, we examined the effects of depleting the 33D1+ DC subset either on the sensitization phase of an allergen or on the induction phase of allergic symptoms via allergen challenge in sensitized mice. …Taken together, our findings suggest that we can manipulate allergic reactions to specific allergens not only by depleting the 33D1+ DC subset, but also by selectively activating the DEC-205+ DC subset through the administration of a glycolipid such as α-GalCer, even during the antigen-specific IgE-producing state.”

2. Differential antigen processing by dendritic cell subsets in vivo.
Dudziak, D., et al.Science. 2007 Jan 5;315(5808):107-11. doi: 10.1126/science.1136080. PMID: 17204652
“RNA was prepared from 33D1 (rat IgG2b, kappa) or αhDEC205 (mouse IgG2b, lambda, clone MG38.2 (18)) hybridoma cells, respectively using RNeasy Midi Kit (Qiagen). …DCs were purified from collagenase treated spleens by negative enrichment with CD19, DX5 and CD90.2 microbeads (Milteny) and incubated with 3µg/ml purified rat 33D1,αDEC205 (NLDC-145), rat IgG2a, and rat IgG2b antibodies for 30 min on ice. …The primary antibodies were B220 A647 (CalTag), purified 33D1 and biotin-anti-mouse DEC205 (the Rockefeller University Monoclonal Antibody Core Facility, New York, New York). …Cells were stained with DEC205-bio, then with CD11c-PE, 33D1-A647 and SAPECy7, and purified by cell sorting into CD11cHigh33D1 and CD11cHighDEC205. …Splenocytes were incubated with 33D1-A647 antibody in the presence of different concentrations of EDTA for 30 min.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse Dendritic Cell Marker 33D1 Monoclonal Antibody (Clone: 33D1) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse Dendritic Cell Marker Monoclonal Antibody (Clone: 33D1), Rat IgG2b Kappa from: In Vivo Grade Recombinant Anti-mouse Dendritic Cell Marker Monoclonal Antibody (Clone: 33D1), Rat IgG2b Kappa: PA007602.r2b

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