Description

PA007799.h1Fs-b: Biotinylated Recombinant Anti-mouse CD8a Monoclonal Antibody (Clone: 53-6.72), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

Our Recombinant Anti-Mouse CD8a Monoclonal Antibody (Clone: 53-6.72) represents a sequence-defined, mammalian-expressed technological upgrade specifically engineered to deliver unmatched signal clarity in flow cytometry (FC/FACS) and immunohistochemistry (IHC-F). As a definitive cell-surface lineage marker, the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain) serves as a critical orchestrator for identifying and gating cytotoxic T lymphocyte (CTL) populations within complex murine models of oncology, autoimmunity, and infectious diseases. By leveraging advanced recombinant engineering, our Anti-Mouse CD8a Clone 53-6.72 retains the exact high-fidelity variable regions of the classic 53-6.7 clone while completely overcoming the biological vulnerabilities of legacy hybridoma-derived reagents. Traditional hybridomas are notoriously prone to genetic drift, unexpected mutations, and contaminating light chains that degrade data quality; in contrast, this premium recombinant anti-mouse CD8a antibody guarantees absolute batch-to-batch consistency and a purified, ultra-pure signal profile, ensuring flawless long-term FACS gating stability.

To maximize analytical performance in multiplex panels and eliminate frustrating background noise during cell surface staining, our platform offers this premium clone in three application-targeted configurations. The classic Rat IgG2a Kappa format provides a trusted, non-conjugated purified framework that aligns perfectly with historical literature controls, making it ideal for customized secondary detection. For sophisticated profiling of myeloid-rich single-cell suspensions, our innovative Human IgG1 Fc Silent™ format features specific mutations engineered to completely abolish non-specific binding to endogenous cellular Fc receptors (FcγR), successfully eliminating tedious Fc-blocking steps and slashing background fluorescence. Furthermore, for ultra-sensitive multi-color panels requiring signal amplification, our site-directed Biotinylated Avi-Tag format introduces a precise biotin-conjugated modification at the C-terminus. Unlike random chemical labeling that frequently blocks the antibody-binding pocket and compromises affinity, our precision Avi-tag technology ensures 100% antigen-binding retention, providing an optimized platform for streptavidin-dye amplification, streptavidin agarose beads, or magnetic bead applications.

Frequently Asked Questions (FAQ) about Recombinant Anti-Mouse CD8a Antibody (Clone: 53-6.72) for Flow Cytometry & IHC：

Q: Is this recombinant anti-mouse CD8a antibody (clone 53-6.72) fully validated for flow cytometry and tissue staining?
Yes. Our recombinant anti-mouse CD8a flow cytometry antibody is strictly optimized and validated for high-resolution single-cell surface immunophenotyping via flow cytometry (FC/FACS). Additionally, it is fully verified for Immunohistochemistry on Frozen sections (IHC-F), allowing researchers to successfully map, gate, and track cytotoxic CD8+ T cell architectures within diverse murine tissue frameworks.

Q: How does the Human IgG1 Fc Silent™ format reduce background noise in multi-color FACS panels?
When profiling myeloid-dense samples (such as spleen or tumor-infiltrating lymphocytes), standard antibodies frequently bind non-specifically to cellular Fc receptors (FcγR), creating false positives. Our Human IgG1 Fc Silent™ Kappa format features targeted engineered mutations that completely abolish FcγR binding interaction. This slashes background fluorescence noise and saves valuable hands-on lab time by eliminating the requirement for complex, tedious Fc-blocking steps during cell surface staining.

Q: What are the biological benefits of site-directed Avi-Tag biotinylation over traditional chemical conjugation?
Traditional chemical biotinylation randomly attaches biotin molecules to lysine residues, which frequently compromises or blocks the antibody’s antigen-binding pocket. Our premium biotin-conjugated variant utilizes precision Avi-tag technology to enforce site-directed biotinylation exclusively at the C-terminus of the heavy chain. This elegant engineering guarantees 100% antigen-binding affinity retention, delivering supreme sensitivity and reproducibility when paired with streptavidin-fluorophore dyes or magnetic beads.

Q: Why does a recombinant 53-6.72 clone generate superior FACS gating results compared to traditional hybridomas?
Legacy hybridoma cell lines are highly vulnerable to genetic drift, unexpected line mutations, or contamination from endogenous, non-specific myeloma light chains over time. Syd Labs’ mammalian-expressed recombinant platform is entirely sequence-defined. Purified to >95% via stringent SDS-PAGE and HPLC assessments, this recombinant mouse CD8a antibody delivers an exceptionally clean signal profile with flawless batch-to-batch consistency, ensuring reproducible FACS gating across multi-year screening cohorts.

Q: Can the non-conjugated formats be seamlessly paired with secondary antibodies for multiplex immunophenotyping?
Yes, absolutely. Both our non-conjugated Rat IgG2a Kappa and Human IgG1 Fc Silent formats are formulated as pristine, preservative-free solutions, making them perfectly suited for customized primary staining panels. They can be effortlessly targeted by host-specific fluorescent secondary antibodies, granting researchers maximum flexibility when building complex, multi-color spectral flow cytometry matrices.