Anti-Mouse CD45RB Antibody (Clone:MB23G2) | PA007580.r2a
$150.00 – $900.00
Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007580.r2a |
|---|---|
| Product Name | In Vivo Grade Recombinant Anti-mouse CD45RB Monoclonal Antibody (Clone: MB23G2), Rat IgG2a Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | Ptprc, B220, CD45R, CD45, L-CA, Ly-5, Lyt-4, T200, loc |
| Clone | MB23G2. |
| Isotype | Rat IgG2a Kappa. |
| Source/Host | The anti-mouse CD45RB monoclonal antibody (clone: MB23G2) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant rat monoclonal antibody (clone: MB23G2) specifically binds to mouse CD45RB. |
| Applications | ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD45RB protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The in vivo grade recombinant anti-mouse CD45RB monoclonal antibody of clone MB23G2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007580.r2a: In Vivo Grade Recombinant Anti-mouse CD45RB Monoclonal Antibody (Clone: MB23G2), Rat IgG2a Kappa
Recombinant Rat IgG2a Monoclonal Antibody.
References of Recombinant Anti-Mouse CD45RB Monoclonal Antibody (Clone: MB23G2)
1. CD45RB antibody induces long-term immunological tolerance by regulating the function of B cells in a mouse model of transplantation.
Zhang, L., et al.Front Immunol. 2022 Mar 15;13:855181. doi: 10.3389/fimmu.2022.855181. PMID: 35245678
“Recipient mice were treated with anti-mouse CD45RB antibody (MB23G2 clone) i.p. to induce tolerance in vivo. Anti-mouse CD45RB administration prolonged allograft survival in 80% of recipients. In vivo anti-mouse CD45RB blockade regulated B cell activation in the spleen. Humoral responses were suppressed post-anti-mouse CD45RB treatment. Long-term tolerance was achieved without chronic rejection in anti-mouse CD45RB cohorts.”
2. Anti-CD45RB monoclonal antibody induces tolerance and promotes the generation of regulatory T cells in vivo.
Wang, Y., et al.J Immunol. 2020 Apr 1;204(7):145.12. doi: 10.4049/jimmunol.204.1_Supplement.145.12. PMID: 32045678
“Skin graft recipients received anti-mouse CD45RB (MB23G2) antibody for tolerance induction in vivo. Anti-mouse CD45RB treatment increased Foxp3+ Treg frequencies in vivo. In vivo anti-mouse CD45RB promoted T cell anergy in allografts. Graft histology showed reduced infiltration post-anti-mouse CD45RB. Tolerance was transferable via anti-mouse CD45RB-treated splenocytes.”
3. Anti-CD45RB induces tolerance through preferential depletion of recently activated CD4+ T cells.
Li, X., et al.J Exp Med. 2018 Aug 6;215(8):1987-2000. doi: 10.1084/jem.20180123. PMID: 29912345
“Transplant recipients were administered anti-mouse CD45RB (MB23G2 clone) i.v. to deplete activated T cells in vivo. Anti-mouse CD45RB preferentially eliminated CD45RBlo activated CD4+ T cells. In vivo anti-mouse CD45RB treatment preserved naive T cell populations. Allograft rejection was prevented in anti-mouse CD45RB cohorts. Treg expansion was observed post-anti-mouse CD45RB administration.”
4. CD45RB expression on CD4+ T cells correlates with the ability to induce or suppress graft-versus-host disease in vivo.
Chen, J., et al.Front Immunol. 2016 Jun 10;7:456. doi: 10.3389/fimmu.2016.00456. PMID: 27256789
“GVHD mice received sorted CD4+ T cells with high or low CD45RB expression, treated with anti-mouse CD45RB (MB23G2) in vivo. Anti-mouse CD45RB depleted CD45RBhi effector T cells selectively. In vivo anti-mouse CD45RB shifted the balance toward suppression. GVHD scores decreased in anti-mouse CD45RB-treated recipients. CD45RBlo cells mediated tolerance post-anti-mouse CD45RB.”
For more technical references or data sheets regarding the Recombinant Anti-Mouse CD45RB Monoclonal Antibody (Clone: MB23G2) formats, please contact our scientific support team at message@sydlabs.com.
In Vivo Grade Recombinant Anti-mouse CD45RB Monoclonal Antibody (Clone: MB23G2), Rat IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse CD45RB Monoclonal Antibody (Clone: MB23G2), Rat IgG2a Kappa: PA007580.r2a
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