Anti-Human / Rat / Fish AChR Antibody (Clone:mAb35) | PA007559.r1

Catalog No.	PA007559.r1
Product Name	In Vivo Grade Recombinant Anti-human/rat/fish AChR Monoclonal Antibody (Clone: mAb35), Rat IgG1 Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Anti-ACHR
Clone	mAb35.
Isotype	Rat IgG1 kappa.
Source/Host	The anti-human/rat/fish AChR monoclonal antibody (clone: mAb35) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant rat monoclonal antibody (clone: mAb35) specifically binds to human/rat/fish AChR.
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human/rat/fish AChR protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-human/rat/fish AChR monoclonal antibody of clone mAb35 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007559.r1: In Vivo Grade Recombinant Anti-human/rat/fish AChR Monoclonal Antibody (Clone: mAb35), Rat IgG1 Kappa

Recombinant rat IgG1 Monoclonal Antibody.

References of Anti-Human AChR Monoclonal Antibody (Clone: mAb35)

1. Treatment of passively transferred experimental autoimmune myasthenia gravis using papain
Tzartos SJ, et al.Immunology. 2000 Jun;100(2):190-7. doi: 10.1046/j.1365-2567.2000.01016.x. PMID: 10792389
“The anti-AChR mAb35 antibody was used to induce experimental autoimmune myasthenia gravis (EAMG) in 4-week-old female Lewis rats via intraperitoneal injection. Papain treatment with mAb35 reduced serum anti-AChR levels within hours, leading to mild weakness and recovery, while untreated mAb35 rats developed severe symptoms and died within 24-30 hours. Papain alone showed no side effects for up to 2 months.”

2. Immunohistochemical localization of nicotinic acetylcholine receptors in the guinea pig bowel and pancreas
Anderson CR, et al.Am J Physiol. 1998 Jan;274(1):G11-20. doi: 10.1152/ajpgi.1998.274.1.g11. PMID: 9450532
“The mAb35 antibody, recognizing alpha-bungarotoxin-insensitive nAChRs, was used for immunocytochemistry in guinea pig gut and pancreas. It revealed receptor clusters on Dogiel type I motorneurons and secretomotor neurons in the bowel, with internal sites in permeabilized ganglia closely associated with ChAT-immunoreactive nerve fibers.”

3. Evidence for spare nicotinic acetylcholine receptors and a beta 4 subunit in bovine adrenal chromaffin cells: studies using bromoacetylcholine, epibatidine, cytisine and mAb35
Campos-Caro A, et al.Br J Pharmacol. 1997 May;121(2):357-64. doi: 10.1038/sj.bjp.0701130. PMID: 9152400
“The mAb35 antibody, targeting nAChRs, caused a time-dependent reduction in Emax for nicotine, epibatidine, and cytisine in bovine adrenal chromaffin cells, indicating a loss of nAChR-mediated secretion. Northern analysis confirmed the presence of a beta 4 subunit transcript, supporting the existence of a nAChR reserve downregulated by mAb35.”

4. Characterization and localization of adrenal nicotinic acetylcholine receptors: evidence that mAb35-nicotinic receptors are the principal receptors mediating adrenal catecholamine secretion
Liang SX, et al.J Neurochem. 1996 May;66(5):2045-55. doi: 10.1046/j.1471-4159.1996.66052045.x. PMID: 8627298
“The mAb35 antibody, recognizing the alpha-subunit of muscle and neuronal nAChRs, reduced nAChR-stimulated catecholamine release in cultured chromaffin cells (IC50 ~10nM). Immunocytochemical studies confirmed specific binding to chromaffin cell sites, with no effect on non-nAChR-mediated release, establishing mAb35’s role in targeting principal adrenal nAChRs.”

For more technical references or data sheets regarding the Anti-Human AChR Monoclonal Antibody (Clone: mAb35) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-human/rat/fish AChR Monoclonal Antibody (Clone: mAb35), Rat IgG1 Kappa from: In Vivo Grade Recombinant Anti-human/rat/fish AChR Monoclonal Antibody (Clone: mAb35), Rat IgG1 Kappa: PA007559.r1