Anti-Human IL-10 Antibody (Clone:JES3-19F1.1.1) | PA007619.r2a
$150.00 – $900.00
Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007619.r2a |
|---|---|
| Product Name | In Vivo Grade Recombinant Anti-human IL-10 Monoclonal Antibody (Clone: JES3-19F1.1.1), Rat IgG2a Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | Interleukin-10, IL-10, CSIF, IL10A, TGIF, B-TCGF, GVHDS, MGC126450, MGC126451 |
| Clone | JES3-19F1.1.1. |
| Isotype | Rat IgG2a Kappa. |
| Source/Host | The anti-human IL-10 monoclonal antibody (clone: JES3-19F1.1.1) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant rat monoclonal antibody (clone: JES3-19F1.1.1) specifically binds to human IL-10. |
| Applications | ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human IL-10 protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The in vivo grade recombinant anti-human IL-10 monoclonal antibody of clone JES3-19F1.1.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007619.r2a:In Vivo Grade Recombinant Anti-human IL-10 Monoclonal Antibody (Clone: JES3-19F1.1.1), Rat IgG2a Kappa
Recombinant Rat IgG2a Monoclonal Antibody.
References of Anti-Mouse IL-10R Monoclonal Antibody (Clone: JES3-19F1.1.1)
1. IL-10R inhibition reprograms tumor-associated macrophages and reverses drug resistance in multiple myeloma.
Vannini C, et al.Nat Cancer. 2024 Sep 5. doi: 10.1038/s43018-024-00815-0. PMID: 39215060
“The anti-IL-10R antibody (clone JES3-19F1.1.1) was used to block IL-10 signaling in the tumor microenvironment of a multiple myeloma mouse model, significantly inhibiting tumor growth in combination therapy. Flow cytometry showed increased M1-like macrophage polarization, and survival curves indicated prolonged median survival compared to controls.”
2. IL-10 constrains sphingolipid metabolism to limit inflammation.
Harris HE, et al.Nature. 2024 Feb 21;626(7999):604-611. doi: 10.1038/s41586-024-07078-0. PMID: 38383790
“The JES3-19F1.1.1 anti-IL-10R antibody, administered intraperitoneally, elevated ceramide levels in colonic tissue of colitis-induced mice. Inflammatory scores decreased with sphingolipid modulation, and gene expression profiling revealed upregulated pathways in treated animals, indicating a role in limiting inflammation.”
3. IL-10R blockade during chronic schistosomiasis mansoni results in the loss of B cells from the liver and the development of severe pulmonary disease.
Wynn TA, et al.PLoS One. 2012 Jan 11;7(1):e30040. doi: 10.1371/journal.pone.0030040. PMID: 22291593
“The JES3-19F1.1.1 antibody neutralized IL-10R during chronic Schistosoma mansoni infection in mice, leading to hepatic B cell depletion 4 weeks post-treatment. Pulmonary inflammation scores increased significantly, with histopathology showing granuloma formation in the lungs of treated mice.”
4. IL-10 restricts activation-induced death of NK cells during acute murine cytomegalovirus infection.
Shellam GR, et al.PLoS One. 2011 Aug 25;6(8):e24500. doi: 10.1371/journal.pone.0024500. PMID: 21849677
“The JES3-19F1.1.1 anti-IL-10R antibody, injected in mice infected with murine cytomegalovirus, reduced viral titers in the spleen and decreased NK cell apoptosis rates. Cytokine profiles shifted towards pro-inflammatory responses, highlighting the antibody’s role in modulating NK cell survival.”
5. IL-10 and TGF-beta redundantly protect against severe liver injury and mortality during acute schistosomiasis.
Wynn TA, et al.J Immunol. 2009 Jan 15;182(2):1150-60. doi: 10.4049/jimmunol.181.2.1150. PMID: 18981143
“Combined JES3-19F1.1.1 and anti-TGF-β antibodies in acute schistosomiasis mouse models reduced liver fibrosis and mortality from 90% to 40%. Liver histopathology showed smaller granulomas in treated cohorts, demonstrating the protective role of dual IL-10R and TGF-β blockade.”
For more technical references or data sheets regarding the Recombinant Anti-Mouse IL-10R Monoclonal Antibody (Clone: JES3-19F1.1.1) formats, or related products from Syd Labs, please contact our scientific support team at message@sydlabs.com.
In Vivo Grade Recombinant Anti-human IL-10 Monoclonal Antibody (Clone: JES3-19F1.1.1), Rat IgG2a Kappa from: In Vivo Grade Recombinant Anti-human IL-10 Monoclonal Antibody (Clone: JES3-19F1.1.1), Rat IgG2a Kappa: PA007619.r2a
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