Anti-human CD16 Antibody (Clone: 3G8) | PA007277.h1Fs

Catalog No.	PA007277.h1Fs
Product Name	In vivo Grade Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 16, Fc gamma RIIIA and Fc gamma RIIIB, Human Fc Receptor Blocking Antibody
Clone	3G8.
Isotype	Human IgG1 Fc Silent Kappa
Source/Host	The anti-human CD16 monoclonal antibody (clone: 3G8) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: 3G8) specifically binds to human and non-human primate CD16.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD16 protein. Human Fc receptor blocking solution, human Fc receptor blocking reagent, and human Fc receptor blocking antibody.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The In vivo Grade Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	The human CD16 Fc blocking antibody (3G8 Fc blocking antibody) is an anti-human Fc receptor antibody for human FcR blocking reagent. Various isotypes are available for certain applications. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007277.h1Fs: Recombinant Anti-human CD16 Monoclonal Antibody (Clone: 3G8), Human IgG1 Fc Silent Kappa, In vivo Grade

Background

The recombinant 3G8 antibody binds to human and non-human primate CD16 or FcγRIII, a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, macrophages, and certain T cells. CD16 consists of Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16.

References of Anti-Human CD16 Monoclonal Antibody (Clone: 3G8)

1. Human blood neutrophils generate ROS through FcγR-signaling to mediate protection against febrile P. falciparum malaria.
Ofori, E. A., et al. Commun Biol. 2023 Jul;6(1):743. doi: 10.1038/s42003-023-05118-0. PMID: 37463969; PMCID: PMC10352520
“Before incubating with merozoites, cells were treated with antibodies against FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), as described previously in ref. 10. Cells were treated for 30 min. at 37 °C with 10 µg/ml of anti-human CD64; anti-human CD32; anti-human CD16, or a combination of these to block FcγR-I, II, and III, respectively. …Blood phagocytes, such as neutrophils and monocytes, generate reactive oxygen species (ROS) as a part of host defense response against infections. …Plasmodium falciparum merozoites opsonized with naturally occurring IgG triggered both intracellular and extracellular ROS generation in blood phagocytes, with neutrophils being the main contributors. …Using specific inhibitors, we show that both FcγRIIIB and FcγRIIA acted synergistically to induce ROS production in neutrophils, and that NADPH oxidase 2 and the PI3K intracellular signal transduction pathway were involved in this process. …Here, we used peripheral blood leukocytes (PBLs) to study ROS production intracellularly and extracellularly using freshly purified P. falciparum merozoites and naturally occurring antibodies from samples from well-established longitudinal cohort studies (LCS) performed in Ghana and India.”

2. Neutralizing antibodies from the rare convalescent donors elicited antibody-dependent enhancement of SARS-CoV-2 variants infection.
Mu, S., et al. Front Med (Lausanne). 2022 Oct;9. doi: 10.3389/fmed.2022.952697. PMID: 36341247; PMCID: PMC9669527
“As SARS-CoV-2 strains continue to evolve, new variants have been increasing in the current global pandemic. …Here, we constructed a series of SARS-CoV-2 variants, including engineered double-mutant variants containing an RBD mutation and D614G in the spike protein and natural epidemic variants, and tested whether plasma samples and top neutralizing antibodies in our laboratory from convalescent patients mediated the ADE effects of these SARS-CoV-2 variants. …Currently, neutralizing antibody and vaccine strategies have been developed by targeting the SARS-CoV-2 strain identified during the early phase of the pandemic. …Only one antibody, 55A8, from the same batch of convalescent patients mediated the ADE effects of multiple SARS-CoV-2 variants in vitro, including six double-mutant variants and four epidemic variants, suggesting that ADE activities may be closely related to the antibody itself and the SARS-CoV-2 variants’ S proteins. …These data suggested that potential neutralizing antibodies may need to undergo ADE screening tests for SARS-CoV-2 variants, which should aid in the future design of effective antibody-based therapies.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD16 Monoclonal Antibody (Clone: 3G8) formats, or related products from Syd Labs, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)

Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry

Anti-human CD16 (3G8) from: Anti-human CD16 Monoclonal Antibody (Clone: 3G8): PA007277.h1Fs Syd Labs