Catalog No.	PA008029.m2cLA
Product Name	Anti-HEL / HEL VHH Mouse IgG2c-LALAPG Kappa Bispecific Isotype Control Antibody (Clone:HyHEL-10 / D3-L11) | PA008029.m2cLA
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Anti-HEL Isotype Control, Recombinant HyHEL-10 Isotype Control, HyHEL-10 Negative Control Antibody, Recombinant HEL D1.3 Isotype Control, HEL D1.3 Negative Control Antibody
Clone	HyHEL-10 / D3-L11.
Isotype	mouse IgG2c, kappa.
Applications	an isotype-matched negative control for VHH and mouse IgG2c kappa bispecific antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The in vivo grade recombinant anti-HEL / HEL bispecific isotype control antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 3 month from date of receipt, 2 to 8°C as supplied. 12 months from date of receipt, -20°C to -70°C as supplied.
Note	Recombinant anti-HEL / HEL mouse IgG2c bispecific isotype control antibodies and anti-HEL / HEL VHH mouse IgG2c bispecific isotype control antibodies good for in vitro and in vivo studies. Low or no specific binding to human, mouse, and rat samples tested.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA008029.m2cLA: In Vivo Grade Recombinant anti-HEL / HEL VHH Mouse IgG2c-LALAPG Kappa Bispecific Isotype Control Antibody

The in vivo grade recombinant anti-HEL / HEL VHH bispecific antibody (mouse IgG2c-LALAPG kappa) was produced in mammalian cells. The second anti-HEL (HEL VHH) is a VHH or nanobody.

Background

A Mouse IgG2c bispecific isotype control antibody is a non-target-specific antibody that matches the host species, isotype, and kappa light chain of a mouse IgG2c bispecific antibody. It is used in various in vitro and in vivo assays like ELISA, Western Blot, Flow Cytometry, and animal model research to differentiate specific antibody binding from non-specific interactions.

The anti-HEL monoclonal antibody (hen egg-white lysozyme) in nature is suitable for use as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The HyHEL-10 / HEL D1.3-based recombinant isotype control and mutants (natural and engineered isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. As an ideal recombinant mouse IgG2c bispecific isotype control antibody, the recombinant HyHEL-10 / HEL D1.3 mIgG2c kappa bispecific isotype control antibody has low or no specific binding to any mouse sample, and completely mouse variable regions and the mouse IgG2c kappa constant regions. The various formats of mouse IgG2c kappa bispecific isotype control antibodies and mutants can be used in ELISA, WB, the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the engineered HyHEL-10 / HEL D1.3 bispecific isotype control mutants, isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the anti-HEL / HEL bispecific isotype control and mutants are available.

References of Mouse IgG2c Bispecific Isotype Control Antibody:

Association and dissociation kinetics of anti-hen egg lysozyme monoclonal antibodies HyHEL-5 and HyHEL-10
Foote J, et al. Biochem J. 1994 Nov 15;304(Pt 1):47-57. doi: 10.1042/bj3040047. PMID: 7818471
The immunoglobulin G1 (IgG1) kappa antibodies HyHEL-5 and HyHEL-10 interact with nonoverlapping epitopes on hen egg lysozyme (HEL); the HyHEL-5/HEL interface has two energetically and structurally important salt links, whereas the HyHEL-10/HEL interface involves predominantly hydrogen bonds and van der Waals interactions. The kinetics of association and dissociation of antibodies HyHEL-5 and HyHEL-10 with HEL under a variety of conditions were investigated in this study. The association of each antibody with HEL follows second-order kinetics. The association process is significantly diffusion-limited, as indicated by the viscosity dependence of the interaction of both antibodies with HEL, although detailed energetics suggest that the association process may be more complex. The association rate constant for the HyHEL-5/HEL system is within a factor of 2 of the modified Smoluchowski estimate for proteins of this size, whereas HyHEL-10 interacts with HEL with an association rate an order of magnitude lower.
Tags: activity of Mouse IgG2c Bispecific Isotype Control; activity of Mouse IgG2c Bispecific Isotype Control mAb

Interaction between the antigen and antibody is controlled by the constant domains: Normal mode dynamics of the HEL–HyHEL-10 complex
Tsuchiya Y, et al. Biophys Chem. 2008 Jul;135(1-3):159-63. doi: 10.1016/j.bpc.2008.03.006. PMID: 18440638
The antigen binding fragment (Fab) of a monoclonal antibody (HyHEL-10) consists of variable domains (Fv) and constant domains (CL–CH1). Normal modes have been calculated from the three-dimensional structures of hen egg lysozyme (HEL) with Fab, those of HEL with Fv, and so on. In the HyHEL-10 system, the crystal structure of HEL–Fv (PDB code: 1C08), which is a complex of HEL and Fv, had no significant differences from that of HEL–Fab (PDB code: 3HFM), which is a complex of HEL and Fab. Figure 1 shows the schematic structure of HEL–HyHEL-10 (Padlan et al. 1989). Fab is composed of the light and the heavy chains (L and H).
Tags: human IgG1 recombinant isotype control; Mouse IgG2c Bispecific Isotype Control antibody for cancer research

Modeling the Binding Sites of Anti-Hen Egg White Lysozyme Antibodies HyHEL-8 and HyHEL-26: An Insight into the Molecular Basis of Antibody Cross-Reactivity and Specificity
Mirsamadi S, et al. Biophys J. 2003 Feb;84(2 Pt 1):1129-36. doi: 10.1016/S0006-3495(03)74740-7. PMID: 12547790
Three antibodies, HyHEL-8 (HH8), HyHEL-10 (HH10), and HyHEL-26 (HH26) are specific for the same epitope on hen egg white lysozyme (HEL), and share >90% sequence homology. The three-dimensional structures of three monoclonal antibodies, D1.3, HyHEL-5, and HyHEL-10, have been studied during binding to different sites on the surface of hen egg white lysozyme (HEL). The antibodies bind to HEL specifically with six upper loops of variable domains (Fv), termed complementary determining regions (CDRs; Ward et al. On the attribution of binding energy in antigen-antibody complexes McPC 603, D1.3, and HyHEL-5. Biochemistry. 1989 May 30;28(11):4735–4749.
Tags: Mouse IgG2c Bispecific Isotype Control for animal model; Mouse IgG2c Bispecific Isotype Control for cancer research

On the attribution of binding energy in antigen-antibody complexes McPC603, D1.3, and HyHEL-5
Novotny J, et al. Biochemistry. 1989 May 30;28(11):4735-49. doi: 10.1021/bi00437a034. PMID: 2775702
On the attribution of binding energy in antigen-antibody complexes McPC 603, D1.3, and HyHEL-5. The HyHEL-10/HEL interface involves predominantly hydrogen bonds and van der Waals interactions. The association rate constant for the HyHEL-5/HEL system is within a factor of 2 of the modified Smoluchowski estimate for proteins of this size. HyHEL-10 interacts with HEL with an association rate an order of magnitude lower. The kinetics of association and dissociation of antibodies HyHEL-5 and HyHEL-10 with HEL under a variety of conditions were investigated in this study.
Tags: Mouse IgG2c Bispecific Isotype Control for mouse tumor model; Mouse IgG2c Bispecific Isotype Control dose

Structure of an antibody-antigen complex: comparison of the crystal structures of a Fab-lysozyme complex and its free Fab
Padlan EA, et al. Mol Immunol. 1989 Mar;26(3):317-27. doi: 10.1016/0161-5890(89)90078-1. PMID: 2784927
The three-dimensional structure of the complex between the Fab fragment of the anti-hen egg white lysozyme monoclonal antibody HyHEL-10 and its antigen has been determined at 2.7 A resolution. HyHEL-10 is a monoclonal antibody raised against hen egg white lysozyme (HEL). The structure of the free Fab fragment of HyHEL-10 has also been determined at 2.0 A resolution. The comparison of the two structures shows that the binding of antigen to antibody induces conformational changes that are propagated from the binding site to the constant domains of

For more references about Mouse IgG2c Bispecific Isotype Control Antibody, please contact our scientific support team with message@sydlabs.com.

Recombinant anti-HEL / HEL Bispecific Isotype Control Antibodies:

In vivo Grade Recombinant anti-HEL / HEL Mouse IgG1 Kappa Bispecific Isotype Control Antibody
In vivo Grade Recombinant anti-HEL / HEL Mouse IgG2a Kappa Bispecific Isotype Control Antibody
In vivo Grade Recombinant anti-HEL / HEL Mouse IgG2c Kappa Bispecific Isotype Control Antibody

Recombinant human IgG Bispecific Isotype Control Antibodies:

In vivo Grade Recombinant Human IgG1 Bispecific Isotype Control Antibody
In vivo Grade Recombinant Human IgG4 Bispecific Isotype Control Antibody

Other Recombinant IgG Reference Antibodies:

In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody

In Vivo Grade Recombinant anti-HEL / HEL VHH Mouse IgG2c-LALAPG Kappa Bispecific Isotype Control Antibody from: In Vivo Grade Recombinant anti-HEL / HEL VHH Mouse IgG2c-LALAPG Kappa Bispecific Isotype Control Antibody: PA008029.m2cLA Syd Labs

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