Recombinant Human TNFa Protein

Catalog No.	BP000123-GD22
Product Name	Recombinant Human TNFa Protein
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Tumor Necrosis Factor-Alpha, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2
Source	E. coli-derived.
Formulation	Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0.
Purity	> 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin	< 0.1 ng/ug (1 EU/ug).
Shipping	The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Note	Conditions of optimal TNFalpha Protein performance should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card.

Description

BP000123-GD22: Recombinant Human TNFa Protein

Background

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF’s primary role is in the regulation of immune cells.

BP000123-GD22: Recombinant Human Tumor Necrosis Factor alpha (TNFα) Protein

Source: E. coli-derived.
Recombinant Human Tumor Necrosis Factor α (TNFα) Protein is a non-glycosylated cytokine produced from E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact bell-shaped homotrimer.
N-terminal sequence analysis: Met-Val-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser-Asp-Lys-Pro-Val- Ala.
Predicted molecular mass: 17,484 Da.
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03 ng/ml. Specific Activity of 3.0 x 10^7 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug). Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0. Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. Other TNF alpha Proteins:
Recombinant Canine TNFα Protein
Recombinant Rat TNFα Protein
Recombinant Human TNFα Protein
Recombinant Human TNFα Protein, HEK
Recombinant Human TNFα Protein
Recombinant Mouse TNFα Protein
Recombinant Human TNFα Protein
Recombinant Rabbit TNFα Protein
Recombinant Mouse TNFα Protein
Recombinant Human TNFα Protein, His Tag
Recombinant Porcine TNFα Protein

TNF alpha Antibodies:
TNFα Monoclonal Antibody
TNFα Polyclonal Antibody
TNFA Polyclonal Antibody
TNFa Polyclonal Antibody
TNFa Polyclonal Antibody

TNF alpha ELISA Kits:
Rat TNFα ELISA Kit
Human TNFα ELISA Kit
Mouse TNFα ELISA Kit

Please remember our product information: Recombinant Human TNFa Protein: BP000123-GD22 Syd Labs

Additional information

Ship from Country	USA
Size	10 ug, 1 mg, 50 ug

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