Catalog No.	BP001020-Z65
Product Name	Recombinant Human p65 Protein
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	transcription factor p65
Source	Baculovirus, SF9 insect cells-derived.
Formulation	25 mM HEPES (pH 7.5), 20% glycerol, 150 mM NaCl, 0.1 mM TCEP 2 mM EDTA, carrier-free.
Purity	> 90%, by SDS-PAGE.
Endotoxin	< 1.0 EU per 1 ug of the protein by the LAL method.
Shipping	The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Note	Conditions of optimal p65 Protein performance should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card.

Description

BP001020-Z65: Recombinant Human p65 Protein

Background

Nuclear Factor kappa B, a transcription factor complex, is ubiquitously expressed in almost all animal cells and involved in cellular responses to various stimuli, such as stress, cytokines, free radicals, bacterial or viral antigens, and etc. Specially, NF-kB plays a key role in the regulation of immune responses. It is kept in cytoplasm by specific inhibitors. Upon degradation of inhibitors after stimulation, NF-kB translocate into nucleus and activate downstream gene expression. NF-kB is composed of NF-kB1 or NF-kB2 bound to either REL, RELA or RELB. The most abundant heterdimeric NF-kB is NF-kB1 complexed with RELA.

BP001020-Z65: Recombinant Human transcription factor p65 protein

Source: Baculovirus, SF9 insect cells-derived.
Predicted molecular mass: 63.6 kDa.
Bioactivity test: 1 unit equals 1 nanogram of purified protein. 10 unit is sufficient for a gel mobility shift assay in a 20 ul reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Purity: > 90%, by SDS-PAGE.
Endotoxin Level: < 1.0 EU per 1 ug of the protein by the LAL method. Formulation: 25 mM HEPES (pH 7.5), 20% glycerol, 150 mM NaCl, 0.1 mM TCEP 2 mM EDTA, carrier-free. Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. Please remember our product information: Recombinant Human p65 Protein: BP001020-Z65 Syd Labs

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