Recombinant SARS-CoV-2 Spike S-His-Avi Protein

Recombinant SARS-CoV-2 Spike S-His-Avi Protein: BP003075 Syd Labs

Recombinant SARS-CoV-2 Spike S-His-Avi Protein

$350.00$2,300.00

In stock

$350.00$2,300.00

Syd Labs Recombinant SARS-CoV-2 Spike S-His-Avi Protein BP003075, cheap recombinant SARS-CoV-2 spike protein (S protein, Val16-Glu1188) expressed in HEK 293 cells with Avi for biotinylation. Biological activity tested. Biotinylated full length 2019-nCoV S protein available.

SKU: BP003075
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Catalog No. BP003075
Product NameRecombinant SARS-CoV-2 Spike S-His-Avi Protein
Supplier Name Syd Labs, Inc.
Brand Name Syd Labs
Synonyms 2019-nCoV Spike Protein; 2019-nCoV S protein; COVID-19 Virus Spike Protein
Source recombinant SARS-CoV-2 spike protein (S protein, Val16-Glu1188) was expressed in mammalian cells with a Fc tag at the C-terminus.
Formulation The recombinant SARS-CoV-2 Spike-Fc protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity > 95% by PAGE under reduced condition, and SEC-HPLC.
Endotoxin Less than 1 EU per ug by the LAL method.
Shipping The recombinant SARS-CoV-2 Spike-Fc protein is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Note Biological activity of the SARS-CoV-2 spike protein (S protein) were tested. His-tagged and Fc-fused recombinant SARS-CoV-2 spike protein subunits and domain, the biotinylated proteins, and anti-spike antibody are available.
Order Offline Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card.

Description

BP003075: Recombinant SARS-CoV-2 Spike S-His-Avi Protein

Background

Source: recombinant SARS-CoV-2 spike protein (S protein, Val16-Glu1188) was expressed in mammalian cells with a Fc tag at the C-terminus.
Accession: QHD43416.1
Predicted molecular mass: 158.3 kDa. Due to glycosylation, the recombinant SARS-CoV-2 S protein migrates to 230-250 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Formulation: The recombinant SARS-CoV-2 Spike-Fc protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.

Shipping: The recombinant SARS-CoV-2 Spike-Fc protein is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

Please ask for a quote if you need Spike protein mutants or an S protein with different modification or components.

SARS-CoV-2 (2019 nCoV or COVID-19 virus) proteins:
Biotinylated Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-Fc-Avi
Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein
Recombinant SARS-CoV-2 Spike S-Fc-Avi Protein
Biotinylated Recombinant SARS Spike RBD-His-Avi
Recombinant SARS Spike RBD-His-Avi
Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi
Biotinylated Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-Fc-Avi Protein

To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services

The SARS-CoV-2 spike (S) glycoprotein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of virus entry inhibitors, neutralizing antibodies, and vaccines. The virus causing Coronavirus Disease 2019 (COVID-19) was named 2019 novel coronavirus (2019-nCoV) or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). There are four major structural proteins in SARS-CoV-2, spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins.

There are two subunits, S1 and S2, in the SARS-CoV-2 spike (S) glycoprotein. The furin cleavage site at the boundary between the S1/S2 subunits is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs. The S1 subunit, the N-terminal 14-685 amino acids of S protein, contains N-terminal domain (NTD), receptor binding domain (RBD), and receptor binding motif (RBM). The C-terminal S2 subunit contains fusion peptide (FP), heptad repeat 1 (HR1), heptad repeat 2 (HR2), transmembrane domain (TM), and cytoplasmic domain (CP). The SARS-CoV and SARS-CoV-2 S proteins mediate viral entry into host cells by binding to a host receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD) in the S1 subunit, and then fusing the viral and host membranes through the S2 subunit. The binding of the S1 subunit to the extracellular peptidase domain of ACE2 on the surface of cells results in endocytosis and translocation of both the virus and the enzyme into endosomes located within cells. This entry process also requires priming of the S protein by the host serine protease TMPRSS2, which is a potential drug for inhibition.

Two trimeric SARS-CoV-2 spike proteins bind to an ACE2 dimer with the collectrin-like domain of ACE2 mediating homodimerization. The ACE2-binding affinity of the Spike RBD of SARS-CoV-2 is 10- to 20-fold higher than that of SARS-CoV. It was also reported that the affinity is similar. The difference in the affinity measurement may result from a variety of factors, such as S and rhACE2 protein fragments used, and measurement methods.

Please remember our product information: Recombinant SARS-CoV-2 Spike S-His-Avi Protein: BP003075 Syd Labs

Additional information

Ship from Country

USA

Size

50 ug, 500 ug

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