Recombinant Human ACE2-Fc-Avi Protein

Catalog No.	BP003062
Product Name	Recombinant Human ACE2-Fc-Avi Protein
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	hACE2, rhACE2, ACE-2, hACE-2, rhACE-2, Angiotensin I Converting Enzyme 2
Source	recombinant human ACE2 protein (Gln18-Ser740) was expressed in mammalian cells with a Fc fusion and Avi at the C-terminus.
Formulation	The recombinant human ACE2-Fc-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity	> 95% by PAGE under reduced condition, and SEC-HPLC.
Endotoxin	Less than 0.5 EU per ug by the LAL method.
Shipping	The recombinant human ACE2-Fc-Avi protein is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Note	His-tagged recombinant human and cyno ACE2 protein (rhACE2-His and rcACE2-His), and the biotinylated ACE2 proteins are available. Please ask for a quote if you need ACE2 protein mutants or an ACE2 protein with different modification or components.
Order Offline	Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card.

Description

BP003062: Recombinant Human ACE2-Fc-Avi Protein

Background

Source: recombinant human ACE2 protein (Gln18-Ser740) was expressed in mammalian cells with a Fc fusion and Avi at the C-terminus.
Accession: Q9BYF1
Predicted molecular mass: 111.2 kDa. Due to glycosylation, the rhACE2 protein migrates to 115-125 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 0.5 EU per ug by the LAL method.
Activity: Immobilized biotinylated recombinant SARS-CoV-2 Spike S RBD with the His Tag at 1 ug/ml (100 ul/well). Dose response curve for rhACE2-Fc fusion protein with the EC50 of 81 ng/ml determined by ELISA. Conditions of optimal recombinant Human ACE2 protein (rhACE2-Fc) performance should be determined experimentally by the investigator.
Formulation: The recombinant human ACE2-Fc-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.

Shipping: The recombinant human ACE2-Fc-Avi protein is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

Questions and answers:

Question: Why did you put an Avi site at the C-terminus of your recombinant human ACE2-Fc-Avi protein (rhACE2-Fc)?
Answer: It is for site-specific biotinylation of the ACE2 protein, and the biotinylated ACE2 proteins are also available.

Question: Did you measure the biological activity of your recombinant human ACE2-Fc-Avi protein?
Answer: Yes. We immobilized biotinylated recombinant SARS-CoV-2 Spike S RBD with the His Tag at 1 ug/ml (100 ul/well), and measure dose response curve for rhACE2-Fc fusion protein with the EC50 of 81 ng/ml by ELISA. Conditions of optimal recombinant Human ACE2 protein performance should be determined experimentally by the investigator.

Question: Can your recombinant human ACE2-Fc-Avi protein be used to measure the function of an anti-S protein neutralizing antibody?
Answer: Yes, the rhACE2 protein binds to the RBD in the S protein S1 subunit.

Question: Do you have any antibody against the human ACE2 protein?
Answer: Yes, it is under development.

SARS-CoV-2 (2019 nCoV or COVID-19 virus) proteins:
Biotinylated Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-Fc-Avi
Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein
Recombinant SARS-CoV-2 Spike S-Fc-Avi Protein
Biotinylated Recombinant SARS Spike RBD-His-Avi
Recombinant SARS Spike RBD-His-Avi
Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi
Biotinylated Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-Fc-Avi Protein

To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services

ACE2 (Angiotensin I Converting Enzyme 2) belongs to the angiotensin-converting enzyme family of dipeptidylcarboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome (SARS) including Coronavirus Disease 2019 (COVID-19), and Neurogenic Hypertension. The SARS-CoV and SARS-CoV-2 (2019-nCoV) spike (S) proteins mediate viral entry into host cells by binding to the ACE2 receptor through the receptor-binding domain (RBD) of the S1 subunit, and then fusing the viral and host membranes through the S2 subunit. The ACE2-binding affinity of the Spike RBD of SARS-CoV-2 is 10- to 20-fold higher than that of SARS-CoV. Two trimeric SARS-CoV-2 spike proteins bind to an ACE2 dimer with the collectrin-like domain of ACE2 mediating homodimerization.

The binding of the S1 subunit to the extracellular peptidase domain of ACE2 on the surface of cells results in endocytosis and translocation of both the virus and the enzyme into endosomes located within cells. This entry process also requires priming of the S protein by the host serine protease TMPRSS2, which is a potential drug for inhibition. The recombinant human ACE2 (rhACE2) may also be a promising drug for those with intolerance to classic renin-angiotensin system inhibitors (RAS inhibitors) or in diseases where circulating angiotensin II is elevated. Infused rhACE2 has been evaluated in clinical trials for the treatment of SARS.

Please remember our product information: Recombinant Human ACE2-Fc-Avi Protein: BP003062 Syd Labs

Additional information

Ship from Country	USA
Size	1 mg, 100 ug

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