Recombinant Human IL-2 Protein

Catalog No.	BP000145-GD20
Product Name	Recombinant Human IL-2 Protein
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Interleukin-2, T-Cell Growth Factor, TCGF, Aldesleukin, IL2
Source	E. coli-derived.
Formulation	Lyophilized from a 0.2 um filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0.
Purity	> 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin	< 0.1 ng/ug (1 IEU/ug).
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Note	Conditions of optimal IL-2 Protein performance should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149Fax: 1-617-606-5019Email: message@sydlabs.comOr leave a message with a formal purchaseorder (PO) Or credit card.

Description

BP000145-GD20: Recombinant Human IL-2 Protein

Background

Recombinant Human interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 human interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the human IL-2 gene, and the resulting expression clone encodes a modified human IL-2. This recombinant form differs from native interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2.

BP000145-GD20: Recombinant Human Interleukin-2 (IL-2) Protein

Source: E. coli-derived.
Recombinant Human Interleukin-2 (IL-2) Protein is expressed with sequence (Pro22-Thr153) of Human IL-2.
N-terminal sequence analysis: Met-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys-Thr-Gln-Leu- Gln-Leu-Glu.
Predicted molecular mass: 15,300 Daltons.
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 1.0 x 10^7 IU/ mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug). Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0. Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. Other IL-2 Proteins:
Recombinant Rat IL-2 Protein
Recombinant Porcine IL-2 Protein
Recombinant Mouse IL-2 Protein, His Tag
Recombinant Mouse IL-2 Protein
Recombinant Human IL-2 Protein, His Tag
Recombinant Human IL-2 Protein, HEK
Recombinant Human IL-2 Protein

IL-2 Antibodies:
IL-2 Polyclonal Antibody
IL-2 Polyclonal Antibody
IL-2 Monoclonal Antibody
IL-2 Monoclonal Antibody
IL-2 Monoclonal Antibody

IL-2 ELISA Kits:
Rat IL-2 ELISA Kit
Mouse IL-2 ELISA Kit
Human IL-2 ELISA Kit
Please remember our product information: Recombinant Human IL-2 Protein: BP000145-GD20 Syd Labs

Additional information

Ship from Country	USA
Size	10 ug, 1 mg, 50 ug

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