Phospho-Histone H3-S10 Rabbit mAb (AP0002)
$148.00 – $548.00
Abclonal Phospho-Histone H3-S10 Rabbit mAb (Catalog Number: AP0002) Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
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| Catalog No. | AP0002 |
|---|---|
| Product Name | Phospho-Histone H3-S10 Rabbit mAb (AP0002) |
| Supplier Name | ABclonal, Inc. |
| Brand Name | Abclonal |
| Synonyms | H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A |
| Gene Name | H3C1 |
| Protein Name | H3C1 |
| Uniprot/Swissprot ID | P68431 |
| Gene ID | 8350 |
| Clone | ARC0003 |
| Clonality | Monoclonal |
| Source/Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Other (Wide Range) |
| Conjugate | Unconjugated |
| Note | Products will be shipped from the warehouse in Massachusetts. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com. |
| Order Offline | Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com |
Description
AP0002: Phospho-Histone H3-S10 Rabbit mAb
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Immunogen Information about Phospho-Histone H3-S10 Rabbit mAb (AP0002)
Immunogen：A synthetic phosphorylated peptide around S10 of human Histone H3 (P68431).
Sequence：ARKST
Gene ID：8290 8350
Swiss prot：Q16695 P68431
Synonyms：H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Phospho-Histone H3-S10
Calculated MW：15kDa
Observed MW：15kDa
Images of Phospho-Histone H3-S10 Rabbit mAb (AP0002)
Western blot analysis of lysates from HeLa, NIH/3T3, C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at 1:1000 dilution. HeLa, NIH/3T3 and C6 cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 1s.
Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded human oophoroma using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded mouse kidney using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.
Confocal imaging of NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of HeLa cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Please remember our product information: Phospho-Histone H3-S10 Rabbit mAb (Catalog Number: AP0002) Abclonal
