Catalog No.	PA008010.h1
Product Name	Human IgG1 Bispecific Isotype Control Antibody (4F17 / HEL D1.3.) | PA008010.h1
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	hIgG1 Isotype Control, hIgG1 D265A N297A, IgG1 LALAPG, IgG1 SELF Mutants
Clone	4F17 / HEL D1.3.
Isotype	human IgG1, kappa.
Applications	an isotype-matched negative control for human IgG1 kappa bispecific antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In Vivo Grade Recombinant Human IgG1 Bispecific Isotype Control Antibody are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 3 month from date of receipt, 2 to 8°C as supplied. 12 months from date of receipt, -20°C to -70°C as supplied.
Note	Recombinant human IgG1 bispecific isotype control antibodies good for in vitro and in vivo studies. Low or no specific binding to human, mouse, and rat samples tested.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA008010.h1: In Vivo Grade Recombinant Human IgG1 Bispecific Isotype Control Antibody

The in vivo grade recombinant human IgG1 bispecific antibody was produced in mammalian cells.

Background

The recombinant human IgG1 bispecific isotype control (hIgG1 kappa bispecific isotype control antibody) and mutants are produced in mammalian cells (HEK293 and CHO cells). The recombinant human IgG1 bispecific isotype control has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG1 kappa constant regions. The human IgG1 kappa isotype control and mutants such as Fc-silenced can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

A number of hIgG1 bispecific isotype control mutants are available. Most of the therapeutic antibody drugs carry the natural human IgG1 constant regions. There are some engineered mutants in the IgG1 Fc fragment. Changes to the IgG Fc sequence including glycosylation sites (such as N297A or N297G) have been used to modulate Fc functions. Even a single mutation may have both enhancement and abrogation effects on Fc binding though multiple mutation combinations are commonly used. We generated hIgG1 bispecific isotype control mutants, the same mutations as those in the therapeutic antibodies and candidates because such human IgG1 isotype control mutants are needed when clients test their engineered antibody candidates. For example, P329G substitution combined with L234A L235A (hIgG1 LALAPG, Fc silenced) was shown to decrease effector functions by disrupting the Fc/Fcγ receptor interface. The silent Fc effctor functions human IgG1 bispecific isotype controls are useful for in vivo use.

Please contact us to ask for a quote for the Fc silenced human IgG1 bispecific isotype control mutants, hIgG1 bispecific isotype control and silent Fc mutants with Avi-, His-, and Flag-tags, and biotinylated bispecific hIgG1 isotype control and Fc silent mutants. A variety of conjugates (such as a fluorophore) with the human IgG1 bispecific isotype control and Fc effector-silent mutants are available.

Recombinant anti-HEL / HEL Bispecific Isotype Control Antibodies:
Recombinant anti-HEL / HEL Mouse IgG1 Kappa Bispecific Isotype Control Antibody, In vivo Grade
Recombinant anti-HEL / HEL Mouse IgG2a Kappa Bispecific Isotype Control Antibody, In vivo Grade
Recombinant anti-HEL / HEL Mouse IgG2c Kappa Bispecific Isotype Control Antibody, In vivo Grade

Recombinant human IgG Bispecific Isotype Control Antibodies:
Recombinant Human IgG1 Bispecific Isotype Control Antibody. In vivo Grade
Recombinant Human IgG4 Bispecific Isotype Control Antibody, In vivo Grade

Other Recombinant IgG Reference Antibodies:
Recombinant Human IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Human IgG2 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG1 Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2a Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2b Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG2c Isotype Control Antibody and Mutants, In vivo Grade
Recombinant Mouse IgG3 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG1 Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2a Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2b Isotype Control Antibody, In vivo Grade
Recombinant Rat IgG2c Isotype Control Antibody, In vivo Grade

In Vivo Grade Recombinant Human IgG1 Bispecific Isotype Control Antibody from: In Vivo Grade Recombinant Human IgG1 Bispecific Isotype Control Antibody: PA008010.h1 Syd Labs

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