CD4 Rabbit mAb (A19018)
$148.00 – $548.00
Abclonal CD4 Rabbit mAb (A19018) encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules.
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| Catalog No. | A19018 |
|---|---|
| Product Name | CD4 Rabbit mAb (A19018) |
| Supplier Name | ABclonal, Inc. |
| Brand Name | Abclonal |
| Synonyms | T4; IMD79; Leu-3; OKT4D; CD4mut |
| Gene Name | CD4 |
| Protein Name | CD4 |
| Uniprot/Swissprot ID | P01730 |
| Gene ID | 920 |
| Clone | ARC0328 |
| Clonality | Monoclonal |
| Source/Host | Rabbit |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Note | Products will be shipped from the warehouse in Massachusetts. Promotion is running from time to time. Welcome to send a request for quote to message@sydlabs.com. |
| Order Offline | Syd Labs, Inc. 4 Avenue E, Hopkinton, MA 01748 USA. Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com |
Description
A19018: CD4 Rabbit mAb
This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Immunogen Information about CD4 Rabbit mAb (A19018)
Immunogen：Recombinant fusion protein containing a sequence corresponding to amino acids 216-396 of human CD4 (P01730).
Sequence：GEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQP
Gene ID：920
Swiss prot：P01730
Synonyms：T4; IMD79; Leu-3; OKT4D; CD4mut; CD4
Calculated MW：51kDa
Observed MW： 55kDa
Images of CD4 Rabbit mAb (A19018)
Western blot analysis of extracts of various cell lines, using CD4 antibody (A19018) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human liver using CD4 Rabbit mAb (A19018) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using CD4 Rabbit mAb (A19018) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using CD4 Rabbit mAb (A19018) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Flow cytometry:1X10^6 K-562 cells (negative control, left) and THP-1 cells (right) were surface-stained with CD4 Rabbit mAb(A19018, 10 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 10 μg/mL, blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:500 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Please remember our product information: CD4 Rabbit mAb (Catalog Number: A19018) Abclonal
