Description

PA007526.r2b: In Vivo Grade Recombinant Anti-Mouse Ter-119 Monoclonal Antibody (Clone: TER-119), Rat IgG2b Kappa

The in vivo grade recombinant anti-mouse Ter-119 rat IgG2b monoclonal antibody was produced in mammalian cells.

Background

Our recombinant TER-119 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse Ter-119 monoclonal antibody (hybridoma clone name or number: TER-119).

References of Anti-Mouse Ter-119 Monoclonal Antibody (Clone: TER-119)

1. Affinity-Restricted Memory B Cells Dominate Recall Responses to Heterologous Flaviviruses.
Wong, R., et al.Immunity. 2020 Nov 17;53(5):1078-1094.e7. doi: 10.1016/j.immuni.2020.09.001. PMID: 33010224
“The following antibodies were purchased from Bio X Cell: M1/70 (CD11b), 2C11 (CD3), GK1.5 (CD4), 53-6.7 (CD8), and TER119. … Splenocytes were stained with rat anti-CD4, CD8, CD11b, CD3, Ter119, IgM, and IgD on ice for 20 min, wash, and then incubated on plates pre-coated with 1 ug/mL of mouse anti-rat IgG (3053-01, Southern Biotech) for 20 min at 4°C. … The non-adherent cell fraction was collected, washed, and then stained with MBC and GC B cell surface markers (CD19, GL7, CD38, CD80, CCR6 and DIII tetramers). … Antigen-specific bone marrow plasma cells were subjected to CD138 enrichment prior to DIII-tetramer staining. … Total bone marrow cells were stained with anti-CD138 APC, cells washed and then stained with anti-PE magnetic beads (0.5 uL/107 cells, Miltenyi Biotec).”

2. Coordinated Viral Control by Cytotoxic Lymphocytes Ensures Optimal Adaptive NK Cell Responses.
Diaz-Salazar, C., et al.Cell Rep. 2020 Sep 22;32(12):108186. doi: 10.1016/j.celrep.2020.108186. PMID: 32966792
“InVivoMab Anti-Mouse Ter-119 (NK cell enrichment, clone TER-119) Bio X Cell Cat#BE0183; RRID:AB_10949625 … Splenic NK cells and/or CD8+ T cells were enriched by negative selection using BioMag Goat Anti-Rat IgG (QIAGEN), mixed with the following Antibody cocktail (anti-CD4 (GK1.5 clone, BioXcell), anti-CD19 (1D3 clone, BioXcell), anti-Ly6G (1A8 clone, BioXcell)), and then sorted to > 95% purity on a BD FACSAria II cell sorter. … Depletion of CD8+ T cells and other lymphocyte populations was achieved by intraperitoneal injection of the following purified antibodies: anti-CD4 (GK1.5 clone, BioXcell, 200 μg/mouse), anti-CD8 (2.43 clone, BioXcell, 100 μg/mouse), or isotype control (LTF-2 clone, BioXcell, 100 μg/mouse) one day prior to infection. … InVivoMab Anti-Mouse CD19 (NK cell enrichment, clone 1D3) Bio X Cell Cat#BE0150; RRID:AB_10949187 … InVivoMab Anti-Mouse Ter-119 (NK cell enrichment, clone TER-119) Bio X Cell Cat#BE0183; RRID:AB_10949625”

3. Cytomegalovirus Infection Drives Avidity Selection of Natural Killer Cells.
Adams, N. M., et al.Immunity. 2019 Jun 18;50(6):1381-1390.e5. doi: 10.1016/j.immuni.2019.04.009. PMID: 31103381
“NK cells (TCR⁻ CD3⁻ CD19⁻ F4/80⁻ CD45+NK1.1+) were enriched from spleens of pooled C57BL/6 mice by negative selection over BioMag goat anti-rat IgG beads (Qiagen) coated with rat anti-mouse CD8α, CD4, CD19, and Ter-119 antibodies (Bio X Cell, clones 2.43, GK1.5, 1D3, and TER-119 respectively) before being sorted to high purity on an Aria II cytometer (BD Biosciences). …Purified splenic NK cells with different Ly49H expression were loaded with 5 μg/mL Fura-2AM for 30 min and transferred into colorless RPMI (no phenol red) containing 5% FCS. 8 × 10⁴ NK cells were mixed 1:4 with Ba/F3-m157 cells and imaged in 8-well chamber slides (Thermo Fisher Scientific) using an inverted fluorescence video microscope (IX-81; Olympus) fitted with a 20 ×, 0.75 NA objective lens (Olympus). …Ly49Hlo and Ly49Hhi NK cells were sorted from the spleens of WT mice 1.5 days post MCMV infection. …Paired-end reads were trimmed for adaptors and removal of low-quality reads using Trimmomatic (v.0.36) (Bolger et al., 2014). …For graphs, data are shown as mean ± SEM, and unless otherwise indicated, statistical differences were evaluated using an unpaired, two-tailed Student’s t test, assuming equal sample variance.”

4. ADAM17 limits the expression of CSF1R on murine hematopoietic progenitors.
Becker, A. M., et al.Exp Hematol. 2015 Jan;43(1):44-52.e1-3. doi: 10.1016/j.exphem.2014.10.001. PMID: 25308957
“ALPs express low levels of CSF1R and differentiate into monocytes in a dose-dependent manner with exogenous M-CSF administration. … CSF1R surface expression was low on ALPs, similar to that seen in LMPPs and markedly less than that observed in CMPs. … Fate decisions during hematopoietic differentiation are regulated by complex interactions between cell-extrinsic and cell-intrinsic cues. At certain intermediates, progenitor cell differentiation in vivo is often more restricted than their epigenetic profiles or in vitro potentials might predict [2,11]. … Indeed, ALPs, along with granulocyte macrophage progenitors, CMPs, and LMPPs, all showed elevated expression of surface CSF1R (Fig. 2; gating strategies shown in Supplementary Figure E1, online only, available at www.exphem.org). … Unexpectedly, ADAM17-deficient ALPs yielded fewer macrophages in vitro than did wild type ALPs at the highest doses of M-CSF.”

5. Maturation and enucleation of primitive erythroblasts during mouse embryogenesis is accompanied by changes in cell-surface antigen expression.
Fraser, S. T., et al.Blood. 2007 Jan 1;109(1):343-52. doi: 10.1182/blood-2006-03-006569. PMID: 16940424
“At E9.5, nearly 90% of EryP/GFP+ cells in the peripheral blood showed low levels of Ter119 staining. … The intensity of Ter119 fluorescence increased over the next 4 days. … By E13.5, EryP/GFP+ cells could be roughly divided into Ter119 med and Ter119 high populations. … From E15.5 to 17.5, essentially all EryP/GFP+ cells expressed Ter119 at high levels. … Ter119 expression was higher in enucleated than in nucleated EryP/GFP+ cells.”

6. Erythroblasts derived in vitro from embryonic stem cells in the presence of erythropoietin do not express the TER-119 antigen.
Otani, T., et al.Exp Hematol. 2004 Jul;32(7):607-13. doi: 10.1016/j.exphem.2004.04.007. PMID: 15246156
“In this serum-free culture, TER-119+ erythroblasts appeared first on day 2 and maturation proceeded until day 7. … When EPO was added to this coculture, the number of induced floating cells increased twofold to threefold. … Unexpectedly, the erythroid-specific antigen TER-119 expression of these cells was drastically reduced. … Our results showed that EPO had the unusual property of inducing TER-119⁻ erythroblasts in ES-derived primitive erythropoiesis. … It is likely that this effect is unique to primitive erythropoiesis.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse Ter-119 Monoclonal Antibody (Clone: TER-119) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse Ter-119 Monoclonal Antibody (Clone: TER-119), Rat IgG2b Kappa from: In Vivo Grade Recombinant Anti-mouse Ter-119 Monoclonal Antibody (Clone: TER-119), Rat IgG2b Kappa: PA007526.r2b