Anti-mouse TCR beta Antibody (H57-597, Armenian hamster IgG)

Catalog No.	PA007523.hs
Product Name	Recombinant Anti-mouse TCR beta Monoclonal Antibody (Clone: H57-597), Armenian hamster IgG
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	H57-597.
Isotype	Armenian hamster IgG
Source/Host	The anti-mouse TCRβ monoclonal antibody (clone: H57-597) was produced in mammalian cells.
Specificity/Sensitivity	The recombinant H57-597 antibody specifically binds to the mouse TCRβ.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	non-conjugated
Purity	>95% by SDS-PAGE under reducing conditions and HPLC
Shipping	The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007523.hs: Recombinant Anti-mouse TCRβ Monoclonal Antibody (Clone: H57-597), Armenian hamster IgG

Recombinant Armenian hamster IgG Monoclonal Antibody with Fc silent mutation.

References of Anti-Mouse TCR beta Monoclonal Antibody for Flow Cytometry (Clone: H57-597)：

1. Anti-TCRβ mAb induces long-term allograft survival by reducing antigen-reactive T cells and sparing regulatory T cells

Miyahara, Y., et al. Am J Transplant. 2012;12(6):1409-1418. PMID: 22420295

“TCR specific antibodies may modulate the TCR engagement with antigen-MHC complexes, and in turn regulate in vivo T cell responses to alloantigens. …In herein, we found that in vivo administration of mAbs specific for mouse TCRβ (H57-597), TCRα or CD3 promptly reduced the number of CD4(+) and CD8(+) T cells in normal mice, but H57-597 mAb most potently increased the frequency of CD4(+) Foxp3(+) Treg cells. …Thus, transient modulation of the TCRβ chain by H57-597 mAb exhibits potent, long-lasting therapeutic effects to control alloimmune responses.”

2. Transient Anti-TCRβ mAb Treatment Induces CD4 + T Cell Exhaustion and Prolongs Survival in a Mouse Model of Systemic Lupus Erythematosus

Gonzalez, N. M., et al. Immunology. 2025;174(2):239-246. PMID: 39648274

“Here, we investigated the therapeutic potential of the anti-TCRβ (H57-597) monoclonal antibody (mAb) in a mouse model of SLE. …Flow cytometric analysis revealed that anti-TCRβ mAb treatment resulted in a reduction in the frequencies of CD4+ T cells and CD138+B220lo/- plasma cells, plus an increase in Foxp3+ regulatory T cell frequency. …Furthermore, CD4+ T cells from anti-TCRβ mAb treated mice exhibited elevated expression levels of PD-1 and TIM-3, with reduced IFN-γ production, indicative of an exhaustion-like phenotype.”

3. Alpha beta T-lymphocyte depleted mice, a model for gamma delta T-lymphocyte functional studies

Carbone, A., et al. Immunol Rev. 1991;120:35-50. PMID: 1830862

“Adult mice can be depleted of essentially all mature alpha beta T lymphocytes by chronic treatment with the framework-recognizing, pan-specific anti-TCR alpha beta mAb, H57-597. …Suppression of alpha beta T-cell development results in the loss of alloantigen reactivity and of B-cell help, suggesting that gamma delta and alpha beta populations differ in their functional capabilities.”

4. Anti-TCRβ mAb in Combination With Neurogenin3 Gene Therapy Reverses Established Overt Type 1 Diabetes in Female NOD Mice

Xie, A., et al. Endocrinology. 2017;158(10):3140-3151. PMID: 28977608

“We investigated whether targeting the T-cell receptor (TCR) with a monoclonal antibody (mAb) abrogates T-cell response against residual and newly formed islets in overtly diabetic nonobese diabetic (NOD) mice. …We found that anti-TCRβ mAb (50 µg/d) reversed >80% new-onset diabetes in NOD mice for >14 weeks by reducing the number of effector T cells in the pancreas.”

5. Monoclonal antibody against T-cell receptor alphabeta induces self-tolerance in chronic experimental autoimmune encephalomyelitis

Lavasani, S., et al. Scand J Immunol. 2007;65(1):39-47. PMID: 17212765

“The therapeutic effect of monoclonal antibody (H57-597 MoAb) against T-cell receptor (TCR) alphabeta has been investigated on MOG(35-55)-induced experimental autoimmune encephalomyelitis (EAE). …Furthermore, anti-TCR alphabeta treatment on an established disease restored the self-tolerance which led to a complete remission of EAE and a dramatic reduction of inflammatory cells in the CNS.”

For more technical references or data sheets regarding the Anti-Mouse TCR beta Monoclonal Antibody for Flow Cytometry (Clone: H57-597) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Related Anti-Mouse TCRβ Recombinant Monoclonal Antibodies (In Vivo Grade):
In Vivo Grade Recombinant Anti-mouse TCRβ Monoclonal Antibody (Clone: H57-597)