Anti-mouse GITR Monoclonal Antibody (Clone DTA-1) | PA007169.m2a
$150.00 – $900.00
In Vivo Grade Recombinant Anti-mouse GITR Mouse IgG2a Lambda Monoclonal Antibody (Clone DTA-1). Recombinant anti-mouse GITR monoclonal antibodies from the variable region sequences of the rat anti-mouse GITR monoclonal antibody (clone number: DTA-1) are produced from mammalian cells and good for in vitro and in vivo studies.
- Details & Specifications
- References
| Catalog No. | PA007169.m2a |
|---|---|
| Product Name | In Vivo Grade Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG2a Lambda |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | Glucocorticoid-Induced TNFR-Related Protein, TNFRSF18; Tumor Necrosis Factor Receptor Superfamily Member 18 |
| Summary | The in vivo grade recombinant anti-mouse GITR mouse IgG2a monoclonal antibody was produced in mammalian cells. |
| Clone | DTA-1. |
| Isotype | mouse IgG2a, lambda. |
| Specificity/Sensitivity | GITR. |
| Applications | Western blot, immunoprecipitation (IP), Flow Cytometry (FC), and various in vitro and in vivo functional assays. |
| Form Of Antibody | 0.2 μM filtered solution of 1x PBS. |
| Endotoxin | Less than 1 EU/mg of protein as determined by LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The in vivo grade recombinant anti-mouse GITR antibodies (clone of DTA-1) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied. |
| Note | Recombinant anti-mouse GITR monoclonal antibodies from the variable region sequences of the rat anti-mouse GITR monoclonal antibody (clone number: DTA-1) are produced from mammalian cells and good for in vitro and in vivo studies. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007169.m2a: Recombinant Anti-mouse GITR Monoclonal Antibody (Clone DTA-1), Mouse IgG2a Lambda, In Vivo Grade
The rat anti-mouse GITR monoclonal antibody DTA-1 (rat IgG2b lambda) reacts with the mouse GITR protein (Glucocorticoid-Induced TNFR-Related protein or tumor necrosis factor receptor (TNFR) superfamily 18, TNFRSF 18). The DTA-1 monoclonal antibody reacts with the mouse GITR which is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes.
Our recombinant DTA-1 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse GITR monoclonal antibody (hybridoma clone name or number: DTA-1).
References of Anti-Mouse GITR Monoclonal Antibody (Clone: DTA-1)
1. Rational design of anti-GITR-based combination immunotherapy.
Zappasodi, et al. Nat Med. 2019 May;25(5):759-766. doi: 10.1038/s41591-019-0420-8. PMID: 31011209; PMCID: PMC7457830
“Glucocorticoid-induced tumor necrosis factor receptor-related protein (GITR) is a promising target for cancer immunotherapy. … Here, we demonstrate that the anti-tumor efficacy of GITR agonism relies on the reduction of intra-tumoral regulatory T (Treg) cells and the activation of CD8+ T cells. … These findings provide a mechanistic rationale for combining anti-GITR with anti-PD-1 or anti-CTLA-4 to overcome resistance to immune checkpoint blockade.”
2. Local Administration of GITR Agonistic Antibody Induces a Stronger Antitumor Immunity than Systemic Delivery.
Wang, et al. Front Immunol. 2019 Mar 20;10:486. doi: 10.3389/fimmu.2019.00486. PMID: 30949170; PMCID: PMC6447616
“Agonistic antibodies targeting the glucocorticoid-induced TNFR-related protein (GITR) have shown promise in preclinical tumor models. … In this study, we demonstrate that local administration of an anti-GITR agonistic antibody (DTA-1) elicits superior antitumor immunity compared to systemic delivery. … The enhanced efficacy of local delivery is associated with increased depletion of regulatory T cells and robust activation of tumor-infiltrating effector T cells in the tumor microenvironment.”
3. Therapeutic antibody activation of the glucocorticoid-induced TNF receptor by a clustering mechanism.
Zhang, et al. Cell Rep. 2022 Feb 22;38(8):110427. doi: 10.1016/j.celrep.2022.110427. PMID: 35196495; PMCID: PMC8880771
“The glucocorticoid-induced TNF receptor (GITR) is a costimulatory immune checkpoint molecule that enhances T cell responses when activated by agonistic antibodies. … Our study reveals that therapeutic anti-GITR antibodies require Fc-gamma receptor (FcγR)-mediated cross-linking to effectively cluster GITR and trigger optimal downstream signaling. … These insights highlight the critical role of antibody clustering mechanisms and provide a framework for engineering next-generation GITR-targeted immunotherapeutics.”
4. Th2 responses to helminth parasites can be therapeutically enhanced by, but are not dependent upon, GITR-GITRL co-stimulation in vivo.
Nair, et al. Infect Immun. 2012 Oct;80(10):3454-3463. doi: 10.1128/IAI.00472-12. PMID: 22825447; PMCID: PMC3407370
“The glucocorticoid-induced tumor necrosis factor receptor (GITR) and its ligand (GITRL) play critical roles in regulating both innate and adaptive immune responses. … Here we investigated the requirement for GITR-GITRL interactions in the development of Th2 immunity against helminth parasites using in vivo murine models. … We found that while endogenous GITR signaling is not strictly required for initiating Th2 responses, therapeutic engagement of GITR using an agonistic antibody can significantly enhance parasite clearance and Th2 cytokine production.”
5. Galectin-9 interacts with PD-1 and TIM-3 to regulate T cell death and is a target for cancer immunotherapy.
Yang, et al. Nat Commun. 2021 Feb 5;12(1):832. doi: 10.1038/s41467-021-21099-2. PMID: 33547300; PMCID: PMC7864927
“Galectin-9 (Gal-9) is a widely expressed mammalian lectin that plays a crucial role in immune tolerance and T cell exhaustion within the tumor microenvironment. … We demonstrate that Gal-9 binds directly to both PD-1 and TIM-3, forming a complex that promotes apoptosis in effector T cells and facilitates tumor immune evasion. … Blockade or modulation of this Gal-9 network, potentially in combination with other immune checkpoint regulators like GITR or PD-L1, represents a promising strategy for synergistic cancer immunotherapy.”
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Anti-mouse GITR Antibody (DTA-1) from : In Vivo Grade Recombinant Anti-Mouse GITR Mouse IgG2a Lambda Monoclonal Antibody(Clone DTA-1): PA007169.m2a Syd Labs
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