Description

PA007561.r2a: In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa

Recombinant rat IgG2a Monoclonal Antibody.

References of Anti-Mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14)

1. Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.
Brinkman, C. C., et al.Nat Commun. 2016 Jun 21;7:12021. doi: 10.1038/ncomms12021. PMID: 27323847
“P values from Wilcoxon signed rank test for pre-injection to control IgG and anti-CD62L; Student’s t-test for control IgG versus anti-CD62L. In all, 1 pre-injection sample, 6 LN from control IgG treated animals and 9 LN from anti-CD62L-treated animals from 1 experiment, representative of 2. …However, there were no differences in the ratio of Lta-/- to WT Treg between control and anti-CD62L-treated LN. …After 18 h 100 µg anti-CD62L (MEL-14, BioXCell) or control Rat IgG2a (2A3, BioXCell) was injected i.v. After 18 h of antibody injection LN and spleen were collected and analysed by flow cytometry. …For multiple comparisons, Tukey post tests were used when all possible comparisons were logically meaningful, and Bonferroni or Dunn’s post tests were used when only certain pairs of comparisons were experimentally or logically possible. …Mouse LN and spleens were passed through 70 µm nylon mesh (Fisher Scientific) to produce single-cell suspensions and were enriched for CD4+ T cells using CD4+ negative selection (Stemcell Technologies).”

2. Naïve T cells re-distribute to the lungs of selectin ligand deficient mice.
Harp, J. R., et al.PLoS One. 2010 Jun 4;5(6):e10973. doi: 10.1371/journal.pone.0010973. PMID: 20532047
“Additionally, as shown later, if we treated recipient mice with blocking antibodies to L-selectin (anti-CD62L), we also observed reductions in naïve CD8 T cells in LNs, and increased T cells in lung and liver. …Generally, when we inhibited naïve CD8 T cell entry to LN, via the HEVs using CD62L antibody blockade, genetic deficiency of selectin ligands, or inhibition of chemokine receptor signaling via G-coupled receptor signaling pathways, we observed more naïve CD8 T cells in the blood, and we also observed more naïve CD8 T cells in non-lymphoid organs. …Moreover, we found that conditions that inhibit T cell trafficking to the LN, and increase the proportion of T cells in the blood, such as Ptx treatment and anti-CD62L blockade, also result in increased naïve CD8 T cell trafficking to non-lymphoid organs. …The importance of elucidating mechanisms by which T cells, as well as other inflammatory cells, migrate into and out of the lung is underscored by the global health burden associated with lung diseases including COPD, asthma, and lung metastasis. …For anti-CD62L experiments, 100 µg of MEL-14 (α-CD62L) blocking antibody (BioXcell, W. Lebanon, NH) was injected i.v. into WT recipient mice.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse CD62L (L-Selectin) Monoclonal Antibody (Clone: MEL-14), Rat IgG2a Kappa: PA007561.r2a